Message - 7th IAL Symposium

More documents

Recommendations

Info

Lichen: from genome to ecosystem in a changing world 4B-P (4B-P7) Submission ID: IAL0230-00002 EARLY DEVELOPMENT OF TEMPERATE FOLIOSE LICHENS ON COVER SLIPS Anstett D. N. 1 , Larsen E. W. 2 1 Ecology and Evolutionary Biology, University of Toronto, Toronto, Ontario, Canada 2 Cell and Systems Biology, University of Toronto, Toronto, Ontario, Canada Lichen developmental biology is of special interest because at least two genomes are involved in morphogenesis. Yet little is known about this process, in part because lichens grow slowly and are notoriously difficult to culture in laboratory conditions. As well, most surfaces used for lichen growth have been difficult to image. We attempt to document lichen development by placing soredia from foliose temperate lichens Parmelia sulcata and Physcia adscendens onto plastic cover slips and growing them outdoors (in Canada 133 km north-east of Toronto) for a period of 15 months. The cover slips allowed non-destructive imaging of live developing lichens as well as preparation for light and electron microscopy. Only soredia seeded on cover slips in May (as opposed to August or January) successfully developed. These samples were analyzed after 6, 10 and 15 months. Welldeveloped attachment hyphae were seen by 6 months (November), while lobules (early lichen thallus) were detected after 10 months. In other words they developed between November and March, Ontario, Canada’s coldest time of the year. This adds weight to previous inferences that temperate lichens are more developmentally competent at cold temperatures. In terms of morphology, the hyphae and lobules varied considerably between species, raising the possibility that lichen species or at least genera may be identifiable at an early point in the development process. Considering spatial patterns, soredia were often found in clumps. Most development occurred on the periphery or outside of the soredial clumps. It was only after 15 months that soredia inside clumps began to form more attachment hyphae and lobules with higher frequency. The delayed development inside clumps suggests these soredia experience lateral inhibition or a lack of nutrients. Microscopic examination suggested that the upper cortex forms after the lower cortex and that it forms in patches. Overall, we have shown that foliose temperate lichens can be cultured on cover slips, presenting a method where development may be observed in identified lichen species. The cover slip technique may be useful for a variety of culture studies including biomonitoring for conditions conducive to lichen development. 192
The 7 th International Association for Lichenology Symposium 2012 (4B-P8) Submission ID: IAL0261-00001 EFFECTS OF UVR (A-B) IN CHLOROPHYLL A FLUORESCENCE OF SEIROPHORA VILLOSA AND RAMALINA PUSILLA AND THEIR ISOLATED PHYCOBIONTS Salva Hernandez G. 1 , Gasulla F. 1 , Guera A. 2 , Barreno E. 1 1 Botany, Universitat de Valéncia - ICBIBE, Valéncia, Spain 2 Plant Biology, University of Alcalá, Alcalá de Henares, Spain The lichens Seirophora villosa (Ach.) Frödén and Ramalina pusilla Le Prévost grow on twigs of shrubs and small trees in coastal dune ecosystems of the Mediterranean area. However, S. villosa grows in microhabitats directly exposed to solar radiation, where R. pusilla does not develop, suggesting a better adaptation to ultraviolet radiation. These two species may be a good model to understand the acclimation of lichens to different types of irradiation. For this reason, we decided to analyze the responses of these lichens and their phycobionts both lichenized and in culture. R. pusilla and S. villosa were collected in Es Trenc (Mallorca, Spain) on Juniperus turbinata Guss. and Pinus halepensis Miller. Phycobionts were isolated and grown on cellulose-acetate discs on agar 3NBBM. The thalli were previously acclimated in growth chambers for 3 days with cycles of 12 h light and 12 h darkness at 17°C. UVA and UVB fluorescent lamps (Philips) was used and located at a precise distance from the samples. The UVA and UVB treatments were done independently. The photosynthetic activity was calculated using the fluorometer DualPam (Walz). The chlorophyll a fluorescence (Cla) measurements were performed after 7, 14 and 28 days (12/12 day/night cycles at 17°C). Algal discs were exposed to UV during 1 h, 2 h, 6 h and 24 h. In S. villosa, maximal PSII quantum yield (Fv/Fm) decline during irradiation time with UVB in both thalli and isolated phycobionts. UVA radiation seems to have low effect on the thalli of S. villosa, and damage was only observed in cultured phycobionts after 24 h of exposure. The experiments with S. villosa showed protection of the algae within the thallus, against UVA and UVB radiation. In R. pusilla a significant effect on Fv/ Fm was observed after 7 days UVA exposure in thalli and 6 h in its isolated phycobionts. Our results show that the phycobionts of S. pusilla are more sensitive to UV radiation than S. villosa. [MCINN (CGL2009-13429-C02- 01/02), AECID (PCI_A_l024755/09) and Generalitat Valenciana (PROMETEO 174/2008 GVA)] 193 4B-P
Page 1 and 2:
The 7 th IAL Symposium 2012 Lichens
Page 3 and 4:
The 7 th International Association
Page 5 and 6:
Welcome Message The 7 th Internatio
Page 7 and 8:
Welcome Message Dear IAL7 Participa
Page 9 and 10:
Report The 7 th International Assoc
Page 11 and 12:
Opening Address The 7 th Internatio
Page 13 and 14:
IAL COUNCIL (2008-2012): The 7 th I
Page 15 and 16:
Page 17 and 18:
SYMPOSIUM VENUE The 7 th Internatio
Page 19 and 20:
FLOOR PLAN The 7 th International A
Page 21 and 22:
Page 23 and 24:
Page 25 and 26:
Page 27 and 28:
Page 29 and 30:
Page 31 and 32:
Page 33 and 34:
Page 35 and 36:
Scientific Program P14
Page 37 and 38:
s s The 7 th International Associat
Page 39 and 40:
Page 41 and 42:
s s s s s s The 7 th International
Page 43 and 44:
s s The 7 th International Associat
Page 45 and 46:
s s s s s The 7 th International As
Page 47 and 48:
s s s The 7 th International Associ
Page 49 and 50:
s s s s The 7 th International Asso
Page 51 and 52:
Page 53 and 54:
Page 55 and 56:
Page 57 and 58:
Page 59 and 60:
Page 61 and 62:
s s s s s s s The 7 th Internationa
Page 63 and 64:
Page 65 and 66:
Page 67:
ABSTRACTS OF CONTRIBUTED PAPERS (OR
Page 70 and 71:
1 2 3
Page 72 and 73:
Lichen: from genome to ecosystem in
Page 74 and 75:
Page 76 and 77:
Page 78 and 79:
Page 80 and 81:
1A-O Lichen: from genome to ecosyst
Page 82 and 83:
Page 84 and 85:
Page 86 and 87:
Page 88 and 89:
Page 90 and 91:
1B-O Lichen: from genome to ecosyst
Page 92 and 93:
Page 94 and 95:
Page 96 and 97:
Page 98 and 99:
2A-1-O Lichen: from genome to ecosy
Page 100 and 101:
Page 102 and 103:
Page 104 and 105:
Page 106 and 107:
Page 108 and 109:
Page 110 and 111:
Page 112 and 113:
Page 114 and 115:
Page 116 and 117:
Page 118 and 119:
Page 120 and 121:
Page 122 and 123:
Page 124 and 125:
3B-1-O Lichen: from genome to ecosy
Page 126 and 127:
Page 128 and 129:
Page 130 and 131:
Page 132 and 133:
Page 134 and 135:
Page 136 and 137:
Page 138 and 139:
Page 140 and 141:
Page 142 and 143:
Page 144 and 145:
Page 146 and 147:
Page 148 and 149:
Page 150 and 151:
Page 152 and 153:
Page 154 and 155:
Page 156 and 157:
Page 158 and 159:
Page 160 and 161:
Page 162 and 163:
Page 164 and 165:
Page 166 and 167:
Page 168 and 169:
Page 170 and 171:
2A-1-P Lichen: from genome to ecosy
Page 172 and 173:
Page 174 and 175:
Page 176 and 177:
Page 178 and 179:
Page 180 and 181:
Page 182 and 183:
Page 184 and 185:
Page 186 and 187:
Page 188 and 189:
Page 190 and 191:
Page 192 and 193:
Page 194 and 195:
Page 196 and 197:
Page 198 and 199:
Page 200 and 201:
Page 202 and 203:
Page 204 and 205:
Page 206 and 207:
Page 208 and 209:
Page 210 and 211:
Page 212 and 213: Lichen: from genome to ecosystem in
Page 236 and 237: 3B-1-P Lichen: from genome to ecosy
Page 276: 1 2 3
show all

Message - 7th IAL Symposium

Create successful ePaper yourself

Delete template?

Save as template?