Message - 7th IAL Symposium

The 7 th International Association for Lichenology Symposium 2012
(3I – P6) Submission ID: IAL0186-00001
ANTIOXIDANT CAPACITY OF PSEUDOEVERNIA FURFURACEA EXTRACT
Ćilerdžić J. 1 , Stajić M. 1 , Vukojević J. 1
1 Institute of Botany, University of Belgrade, Faculty of Biology, Belgrade, Serbia
Evaluation of the antioxidant capacity of ethanol extract of Pseudoevernia furfuraceae (L) Zopf. was
the aim of this study. P. furfuraceae originated from Tara Mountain (Serbia) was used for the study. 5.5 g of
air dried sample was extracted by stirring with 30-fold higher volume of 70% ethanol at 30 º C for 72 hours and
filtering through Whatman No. 4 filter paper. The obtained ethanol extract was evaporated at 40 º C to dryness,
redissolved in 96% ethanol to a final concentration of 20 mg/ml. Antioxidant capacity was determined spectrophotometrically
by methanol solution of DPPH at 517 nm. Antioxidant activity of the tested extract concentrations
were studied in comparison to commercial antioxidant, BHA. The concentrations of phenol and flavonoid compounds
in the extract were measured spectrophotometrically by Folin-Ciocalteu reagent at 740 nm and ethanol/
aluminium nitrate/potassium acetate mixture at 415 nm, respectivelly. Tests were carried out by various extract
dilutions, in triplicate. Six extract concentrations ranged from 10 mg/ml to 0.312 mg/ml, obtained by double
disolutions, caused decrease of DPPH free radical-scavenging in the range from 90.04% to 21.39%. Defined
EC 50 value was 1.85 mg/ml. The tested extract concentrations had strong antioxidant activity compared to BHA
one (92.61%). The amounts of total phenol compounds were ranged from 61.53 µg/ml (10 mg/ml) to 5.540 µg/
ml (1.25 mg/ml), while at lower extract concentrations these compounds were missed. The total flavonoids content
was significantly higher, ranged from 240.13 μg/ml (10 mg/ml) to 62.97 μg/ml (0.625 mg/ml), while at the
concentration of 0.312 mg/ml their presence was not noted. Although, antioxidant activity had some value at the
concentration of 0.312 mg/ml, it could be concluded that other compounds were responsible for it. Scavenging
effect directly correlates with the different flavonoid content (r 2 = 0.9641) and less with phenol compounds content
(r 2 = 0.6826).
(3I – P7) Submission ID: IAL0236-00001
ANTIFUNGAL ACTIVITY AGAINST PLANT PATHOGENIC FUNGI FROM CRUDE EXTRACT OF
USNEA PULVINULATA
Pengproh R. 1 , Papong K. 1 , Sangdee A. 1 , Chantiratikul P. 2
1 Biology, Mahasarakham University, Mahasarakham, Thailand
2 Chemistry, Mahasarakham University, Mahasarakham, Thailand
Usnea pulvinulata contains various secondary metabolites such as usnic acid, diffractaic acid, menegazziaic
acid, norstictic acid, protocetraric acid, salazinic acid and ursoric acid. This study tested the inhibition
of crude extracts of U. pulvinulata from absolute water, acetone, ethanol and methanol against 6 plants pathogenic
fungi such as Colletotrichum gloeosporiodes (banana anthracnose, cultivated banana anthracnose, chili
anthracnose, Dendrobium anthracnose and mango anthracnose), Colletotrichum capsici (chili anthracnose and
papaya anthracnose), Curvularia lunata (dirty panicle disease of rice), Diplodia sp. (leaf blight of Dendrobium),
Fusarium monilifore (bakanea disease of rice) and Pestalotiopsis guepinii (leaf blight of Guava). The crude extract
of U. pulvinulata from acetone showed the highest inhibition against all six plants pathogenic fungi. Ethanol
and methanol crude extracts can be antifungal against plant pathogen fungi. The crude extract from absolute
water, however, did not inhibit the tested fungi.
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