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Applied Biosystems SOLiD™ 4 System SETS Software User Guide ...

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5<br />

Chapter 5 Perform Reanalysis<br />

Secondary reanalysis<br />

Secondary reanalysis<br />

Overview Secondary reanalysis starts at the Single Tag Analysis stage. This<br />

workflow stage generates application-specific results such as base<br />

space sequences, alignment, tag counting from the colorspace<br />

sequence reads, and ".csfasta" file. The external analysis pipelines<br />

will also be executed if they are plugged-in and turned-on in the<br />

selected analysis setting.<br />

The secondary reanalysis allows you to selectively apply secondary<br />

analysis settings to individual samples. You are not forced to<br />

reanalyze all samples as you are in primary reanalysis. The<br />

downstream effect is that a new analysis is created. All Single Tag<br />

analyses are rerun.<br />

To perform a secondary reanalysis:<br />

1. Click Reanalyze Secondary to<br />

reanalyze the secondary analysis data.<br />

Click the icon for more information.<br />

100 <strong>Applied</strong> <strong>Biosystems</strong> SOLiD 4 <strong>System</strong> <strong>SETS</strong> <strong>Software</strong> <strong>User</strong> <strong>Guide</strong>

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