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Applied Biosystems SOLiD™ 4 System SETS Software User Guide ...

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D<br />

Appendix D Advanced Topic: Primary Analysis<br />

Primary analysis workflow<br />

BC Primary analysis statistics file (.stats) for library<br />

$sampleResultFolder/libraries/{library.name}/primary.[17<br />

-<br />

digittimestamp]/reads/{run.name}_{sample.name}_BC.stats<br />

Reads that have less than expected read length or duplicated<br />

$sampleResultFolder/primary.[17-digit timestamp]/reject<br />

Reads that have less than expected read length or duplicated for<br />

library<br />

$sampleResultFolder/libralies/{library.name}/primary.[17<br />

-digit timestamp]/reject<br />

Reads without barcode reads<br />

$sampleResultFolder/libraries/{library.name}/missing-bc<br />

$sampleResultFolder/libraries/{library.name}/missing-f3<br />

Reads that could not be assigned to a library<br />

$sampleResultFolder/libraries/{library.name}/unassigned<br />

Barcode assignment statistics file/report<br />

$sampleResultFolder/libraries/BarcodeStatistics. [17digittimestamp].txt<br />

168 <strong>Applied</strong> <strong>Biosystems</strong> SOLiD 4 <strong>System</strong> <strong>SETS</strong> <strong>Software</strong> <strong>User</strong> <strong>Guide</strong>

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