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Applied Biosystems SOLiD™ 4 System SETS Software User Guide ...

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Key files generated by primary analysis<br />

xxx_sequence.csfasta<br />

<strong>Applied</strong> <strong>Biosystems</strong> SOLiD 4 <strong>System</strong> <strong>SETS</strong> <strong>Software</strong> <strong>User</strong> <strong>Guide</strong><br />

Appendix D Advanced Topic: Primary Analysis<br />

Key files generated by primary analysis D<br />

Table 6 Key files generated by primary analysis<br />

The “.csfasta” file is a color-space fasta file that contains the color<br />

calls generated for each tag, with the last base of the primer<br />

prepended.<br />

The format is:<br />

>TAG_ID<br />

Color_space<br />

For example:<br />

>1_88_1830_R3<br />

G32113123201300232320<br />

>1_89_1562_R3<br />

File name Description<br />

xxxx_sequence.csfasta The raw data from all beads<br />

xxxx_sequence.QV.qual The quality values file<br />

.spch files Cache files for primary analysis<br />

G23133131233333101320<br />

This file contains all the data that passed filtering, for complete reads<br />

(for example, 35 base read has 35 bases). This file is the input to the<br />

alignment tool.<br />

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