Applied Biosystems SOLiD™ 4 System SETS Software User Guide ...

Applied Biosystems SOLiD 4 System SETS Software User Guide
Appendix D Advanced Topic: Primary Analysis
Procedures to verify primary analysis status D
3. Log in to the instrument and look in the following directory:
{SampleResultsFolder}/primary.{17-digit timestamp}/reads
There should be a file named {run.name}_F3.stats for F3 tag,
or {run.name}_R3.stats for R3 tag. This file contains
information about the color-space reads generation for each
panel. It contains the number of reads and an indication of
whether they have too many errors, are too short, or are
duplicates.
The Totals (### p) line summarizes the number of panels found
and the percentage of error, short, and duplicate reads.
After this line is a line beginning with Usable (### p) (##%).
This line summarizes how many panels were usable and the
percentage of erroneous, short, or duplicate reads among the
usable panels.
If these two lines are missing, the primary analysis did not
complete.
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