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Applied Biosystems SOLiD™ 4 System SETS Software User Guide ...

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.spch files<br />

<strong>Applied</strong> <strong>Biosystems</strong> SOLiD 4 <strong>System</strong> <strong>SETS</strong> <strong>Software</strong> <strong>User</strong> <strong>Guide</strong><br />

Appendix D Advanced Topic: Primary Analysis<br />

Key files generated by primary analysis D<br />

Results of primary analysis for each panel are stored in files with the<br />

".spch" extension (Solid Panel Cache HDF5). The ".spch" file is<br />

used as a cache; that is, primary analysis both writes results to the<br />

file and reads results from the file as analysis proceeds on a cycleby-cycle<br />

basis. Data within the ".spch" file is organized by cycle.<br />

When all cycles have been analyzed, a final processing step converts<br />

the data from the cycle-based format to the “read-based” ASCII<br />

formats (“.csfasta” and “.qual”).<br />

".spch" files use the hierarchical binary format HDF5. Details on this<br />

format are available at:<br />

http://www.hdfgroup.org/HDF5/.<br />

Tools to view the contents and extract results are available at:<br />

http://www.hdfgroup.org/hdf-java-html/hdfview/<br />

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