Applied Biosystems SOLiD™ 4 System SETS Software User Guide ...

Auto-Correlation
Report
Applied Biosystems SOLiD 4 System SETS Software User Guide
Chapter 4 View Reports
View Analysis Reports 4
With quality values, it is possible to predict the expected number of
mismatches to the reference genome caused by measurement errors
as depicted in the bar chart above. The blue line shows the
accumulative read proportion with predicted mismatches. For this
run example, about 28% of the color space calls are predicted to have
0 to 0.5 mismatches. About 80% of the bases are predicted to have
approximately 0 to 3 mismatches.
The final graph included with the Quality Values Report shows the
proportions of six groups of quality values versus the primers and
cycles in the run. The proportion of the highest quality bases tend to
decrease in later cycles and increase after the primer resets. (PAC1
represents Primer A Cycle 1, PBC5 represents Primer B Cycle 5).
Use this report to view the correlation between pairs of color calls in
the raw reads.
Click Auto Correlation Report (Run Details�Analysis Reports).
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