Applied Biosystems SOLiD™ 4 System SETS Software User Guide ...

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Chapter 6 Support Multiplexing
Multiplexing Assignment Report
Import a
Multiplexing Series
The Multiplexing Assignment report shows the number of barcode
reads assigned to each library, along with the subtotals for all
libraries. It also shows the number of reads that were not assigned to
any specified library. These numbers include both passed and failed
reads from the filter fasta stage.
The value in the “0 Mismatch” column is the number of barcode
reads that match exactly to the barcode shown in the barcode
column. The “1 Mismatch” column is the number that missed
matching that barcode by one.
Reads that did not match any of the barcodes at the specified level
are counted in the unassigned library with the name unresolved. It is
possible that barcodes in the kit that were not assigned to a library
are also matched. In that case, the unassigned library may show
barcodes named with the color sequence of the matched barcode.
Two additional libraries may appear:
For beads that have barcode read but no F3 read (missing-f3).
For reads that have an F3 read but no barcode read 9missingbc).
The “Total % Assigned” is the sum of the library subtotals divided
by the “All Beads Total” expressed as a percent.
Follow the steps that use the Command line to import a multiplexing
series found in the Applied Biosystems SOLiD 4 System ICS
Software Help.
Multiplexing report Multiplexing report can be attained as soon as barcoding is complete
for a run.
110 Applied Biosystems SOLiD 4 System SETS Software User Guide