Applied Biosystems SOLiD™ 4 System SETS Software User Guide ...
Applied Biosystems SOLiD™ 4 System SETS Software User Guide ...
Applied Biosystems SOLiD™ 4 System SETS Software User Guide ...
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Storage requirements for primary analysis<br />
Table 5 Primary analysis storage requirements<br />
<strong>Applied</strong> <strong>Biosystems</strong> SOLiD 4 <strong>System</strong> <strong>SETS</strong> <strong>Software</strong> <strong>User</strong> <strong>Guide</strong><br />
Appendix D Advanced Topic: Primary Analysis<br />
Primary analysis workflow D<br />
The storage requirements values given in the table below assume a<br />
50-base tag run in one flowcell. If you use a tag size that is larger or<br />
smaller than 50 bases, the storage requirements differ from those<br />
given in Table 5.<br />
Files Storage required Notes about archiving<br />
.csfasta 56 - 80 GB Should be archived.<br />
_QV.qual 112 - 140 GB Should be archived.<br />
.spch 646 GB Should be archived for the<br />
lowest-level non-image backup.<br />
An estimate for the disk space<br />
needed for one ".spch" file (per<br />
panel) is n*(18*m+17) bytes,<br />
where n is the number of beads,<br />
and m is the number of cycles (F3<br />
and R3).<br />
For 50 cycles (1x50) and 300K<br />
beads, this yields 275 MB (per<br />
panel).<br />
A full slide has 2357 panels,<br />
which means that 646 GB are<br />
required for a full slide (50 bp<br />
single tag run).<br />
postPrimerSetPrimary.[JobID] 364 - 420 GB Temporary files. Most of these are<br />
removed upon completion.<br />
Intensity files 4 x 364 - 420 = 1344 - 1680 GB Optional. Not generated by<br />
default. It is strongly<br />
recommended that intensity files<br />
be generated with offline<br />
ReadBuilder, which is part of the<br />
BioScope <strong>Software</strong>.<br />
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