Applied Biosystems SOLiD™ 4 System SETS Software User Guide ...

Error Profiles
Report
Applied Biosystems SOLiD 4 System SETS Software User Guide
Chapter 4 View Reports
View Analysis Reports 4
The Same data as the one on the previous page but sorted in the order
of the chemistry cycles on the sequencing instrument.
Light blue shading or dark red shading shows stronger correlations
between the two cycles. Such correlations indicate potential
problems with swapped reagents, incomplete cleavage, and other
sequencing chemistry problems.
The Error Profiles Report gives the frequency of errors as a function
of base position in the read and type of error produced for a
particular tag.
Click the Error Profiles Report link (Run Details Analysis Reports)
to view the graphs that show the number of mismatches to the
reference sequence, shown by position on read (top) and by cycle
(bottom). Mouse over to view the calculated values for each bar.
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