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Applied Biosystems SOLiD™ 4 System SETS Software User Guide ...

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3<br />

Chapter 3 Monitor the Run in <strong>SETS</strong><br />

View Run Metrics<br />

2. Select the primer set, primer, cycle, and version you want to<br />

monitor, then click Display to update the traffic lights.<br />

3. Determine the quality of your run by looking at the traffic light<br />

indicators for in-progress runs.<br />

Green - when a traffic light for a metric displays green, that<br />

parameter has a passing quality value and is hitting its target<br />

threshold.<br />

Yellow - when a traffic light for a metric displays yellow, that<br />

parameter has a value that falls between the low and high<br />

threshold and is questionable in quality.<br />

Red - when a traffic light for a metric displays red, that<br />

parameter has a value that is above the high threshold limit and<br />

the quality could be considered poor enough to fail; if a sample<br />

displays all red traffic lights during a run, you may want to abort<br />

that run immediately.<br />

4. Click Get summary when you want a full summary of all the<br />

metrics for an entire run. Get latest status displays by default.<br />

44 <strong>Applied</strong> <strong>Biosystems</strong> SOLiD 4 <strong>System</strong> <strong>SETS</strong> <strong>Software</strong> <strong>User</strong> <strong>Guide</strong>

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