Applied Biosystems SOLiD™ 4 System SETS Software User Guide ...

4
Chapter 4 View Reports
View overall run reports
Bead definitions
Bead Type Definition
Usable Beads Beads that, in a particular ligation cycle, are mapped in
each of the four images. Typically, most beads in a
panel are usable.
The ones that are not lie near the edge of the panel,
which for current purposes is defined as the area
imaged by the corresponding reference or focal map
image.
The ligation fluorescence images must be shifted a few
to tens of pixels to register them with the reference
image. If, for example, a ligation image must be shifted
10 pixels to the left to register with the reference image,
beads within 10 pixels of the right edge of the panel will
not be mapped in that ligation image.
Another common case is a panel-cycle where one of
the ligation images fails to register. In this case, none of
the beads are usable.
Good Beads Usable beads that satisfy all of the following conditions:
intensity >0.05
0.0125 < angle ≤0.05
Best Beads Usable beads that satisfy all of the following conditions:
intensity >0.05
angle ≤0.0125
68 Applied Biosystems SOLiD 4 System SETS Software User Guide