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Applied Biosystems SOLiD™ 4 System SETS Software User Guide ...

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D<br />

What is primary analysis?<br />

<strong>Applied</strong> <strong>Biosystems</strong> SOLiD 4 <strong>System</strong> <strong>SETS</strong> <strong>Software</strong> <strong>User</strong> <strong>Guide</strong><br />

Appendix D<br />

Advanced Topic: Primary<br />

Analysis<br />

The topics provided in this appendix are intended for advanced users<br />

of the SOLiD 4 <strong>System</strong> and do not apply to the typical user.<br />

Primary analysis is the process of extracting color calls or<br />

assignments (.csfasta file) from images captured from a sequencing<br />

run, calculating a quality value for each color call, and writing the<br />

color-call value to a QV file (.qual).<br />

Primary analysis also provides real-time feedback about a<br />

sequencing run.<br />

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