Applied Biosystems SOLiD™ 4 System SETS Software User Guide ...

More documents

Recommendations

Info

6 Chapter 6 Support Multiplexing Multiplexing Assignment Report Multiplexing Assignment Report Reports for Multiplexing Series runs To view multiplexing run analysis results, select the sample folder�analysis folder�library folder. Note: Enter a library name in the Search field to automatically expand the sample tree to that library. There is a wide range of reports that you can generate from the results data, one of which is the Multiplexing Assignment Report. 108 Applied Biosystems SOLiD 4 System SETS Software User Guide
Multiplexing Assignment report Applied Biosystems SOLiD 4 System SETS Software User Guide Chapter 6 Support Multiplexing Multiplexing Assignment Report 6 The Multiplexing Assignment report is a report specific to barcode runs. There is one report generated per analysis. When you have selected your target analysis folder, click . 109
Page 1 and 2:
Applied Biosystems SOLiD 4 System
Page 3 and 4:
Page 5 and 6:
Page 7 and 8:
How to use this guide Applied Biosy
Page 9 and 10:
1 �� SETS So
Page 11 and 12:
Naming convention for sequencing re
Page 13 and 14:
SETS homepage Applied Biosystems SO
Page 15 and 16:
Page 17 and 18:
2 �� SETS So
Page 19 and 20:
SOLiD 4 System Software workflow A
Page 21 and 22:
Log in to SETS Applied Biosystems S
Page 23 and 24:
Define primary analysis settings Ap
Page 25 and 26:
Primary analysis parameters 5. Clic
Page 27 and 28:
Parameter Description Applied Biosy
Page 29 and 30:
Parameter Description Applied Biosy
Page 31 and 32:
Parameter Description Application D
Page 33 and 34:
Define secondary analysis settings
Page 35 and 36:
Chapter 2 Prepare Run Settings Set
Page 37 and 38:
Load a Reference Sequence Specify a
Page 39 and 40:
Page 41 and 42:
3 �� SETS So
Page 43 and 44:
Note: For in-progress runs, you can
Page 45 and 46:
Note: Refer to the Imaging Metrics
Page 47 and 48:
Chapter 3 Monitor the Run in SETS V
Page 49 and 50:
View sample data Applied Biosystems
Page 51 and 52:
Troubleshoot failed jobs Sample ana
Page 53 and 54:
Colorcall Jobs Applied Biosystems S
Page 55 and 56:
Filter Fasta fails Applied Biosyste
Page 57 and 58: View History View Run History in SE
Page 59 and 60: Applied Biosystems SOLiD 4 System
Page 61 and 62: System Logs Applied Biosystems SOLi
Page 63 and 64: 4 �� SETS So
Page 65 and 66: Imaging Metrics Report Applied Bios
Page 67 and 68: Cycle Heat Map Report Select a heat
Page 69 and 70: Chapter 4 View Reports View overall
Page 71 and 72: Exposure Time Report Applied Biosys
Page 73 and 74: Chapter 4 View Reports View overall
Page 75 and 76: Determine the optimal titration poi
Page 77 and 78: View sample reports Applied Biosyst
Page 79 and 80: Resizing images Applied Biosystems
Page 81 and 82: Scaled data Applied Biosystems SOLi
Page 83 and 84: Angle data Applied Biosystems SOLiD
Page 85 and 86: View Analysis Reports Applied Biosy
Page 87 and 88: View analysis Data Files View Analy
Page 89 and 90: Auto-Correlation Report Applied Bio
Page 91 and 92: Error Profiles Report Applied Biosy
Page 93 and 94: Master Report Tool Generate a Maste
Page 95 and 96: Chapter 4 View Reports Master Repor
Page 99 and 100: Chapter 5 Perform Reanalysis Primar
Page 101 and 102: Effect on the current run Applied B
Page 105 and 106: View Multiplexing Series in SETS Ac
Page 107: Set up Multiplexing run in ICS Crea
Page 111 and 112: Reassign Run Applied Biosystems SOL
Page 115 and 116: Auto export in SETS Applied Biosyst
Page 117 and 118: Auto export with JMS broker and Exp
Page 119 and 120: 3. Run the following command: Appli
Page 121 and 122: Manually configure the RSA keys App
Page 123 and 124: Change export for current run only
Page 125 and 126: Event notifications and email servi
Page 129 and 130: Delete runs Applied Biosystems SOLi
Page 131 and 132: Chapter 7 Manage Administrative Tas
Page 133 and 134: A Overview Applied Biosystems SOLiD
Page 135 and 136: Appendix A Web Services WSDL A Bind
Page 137 and 138: B Applied Biosystems SOLiD 4 Syste
Page 139 and 140: Appendix B Advanced Topic: Data Ana
Page 145 and 146: Appendix B Advanced Topic: Data Ana
Page 147 and 148: Complementing color-space data Appl
Page 151 and 152: Data analysis considerations Unders
Page 153 and 154: SNPs and errors High accuracy for S
Page 155 and 156: Sampling Allele ratio Applied Biosy
Page 157 and 158: C Saving data Applied Biosystems SO
Page 159 and 160:
Page 161 and 162:
D What is primary analysis? Applied
Page 163 and 164:
Primary analysis workflow Applied B
Page 165 and 166:
Primary analysis inputs and outputs
Page 167 and 168:
Appendix D Advanced Topic: Primary
Page 169 and 170:
Storage requirements for primary an
Page 171 and 172:
Page 173 and 174:
Page 175 and 176:
Redo the read-filtering process App
Page 177 and 178:
Image metrics Applied Biosystems SO
Page 179 and 180:
Page 181 and 182:
Usage Validation and observed quali
Page 183 and 184:
Key files generated by primary anal
Page 185 and 186:
.spch files Applied Biosystems SOLi
Page 187 and 188:
Troubleshooting analysis failure Ap
Page 189 and 190:
Page 191 and 192:
E Applied Biosystems SOLiD 4 Syste
Page 193 and 194:
LICENSE Appendix E Software License
Page 195 and 196:
Appendix E Software License Agreeme
Page 197 and 198:
Page 199 and 200:
Page 201 and 202:
Alignment Matrix The alignment matr
Page 203 and 204:
Page 205 and 206:
Glossary WFA A Workflow Analysis (W
Page 207 and 208:
Related documentation Document BioS
Page 209:
Part Number 4448411 Rev. B 04/2010
show all

Applied Biosystems SOLiD™ 4 System SETS Software User Guide ...

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?