Applied Biosystems SOLiD™ 4 System SETS Software User Guide ...

Filter Fasta fails
Applied Biosystems SOLiD 4 System SETS Software User Guide
Chapter 3 Monitor the Run in SETS
Troubleshoot failed jobs 3
The Filter_Fasta job details will show a red icon indicating that the
job has failed.
This problem can occur either at the Filter Fasta stage or at the
Barcording stage due to a bad hard-drive, a lack of disk space, or
other system-related issues.
The software displays an error message and provides the Bead ID or
Sequence number where the record is corrupted.
Example
Based on the original system-related issue, you will see an error
message indicating that the “Fasta is empty” or the “Format is
not right expecting sequence. Current line - >1_10_117_F3.”
Click OK to continue.
The error message will contain the problematic file’s location and
record information.
To resolve, you need to fix any system-related issues first. Then, any
of the following step may be applicable:
Check the corrupted Fasta File manually; if possible fix it from
the command line.
Reanalyze primary analysis stage through SETS or manually by
changing the database record/flag.
If the SPCH files are also corrupted, report the error message to
the Applied Biosystems Field Support Engineer.
Note: Report any critical error messages to the
Applied Biosystems FSE.
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