Applied Biosystems SOLiD™ 4 System SETS Software User Guide ...

Image metrics
Applied Biosystems SOLiD 4 System SETS Software User Guide
Appendix D Advanced Topic: Primary Analysis
Image metrics D
Image metrics are measures of the quality of the images produced by
the SOLiD 4 Analyzer. The principal image metrics are the blur
metric and the exposure metric.
Blur metric is a real number greater than zero that provides a
measure of the average apparent radius of the beads in an image. The
blur metric is computed for every image that is analyzed by primary
analysis. The blur metric supports diagnosing instrument focusing
and vibration issues. Figure 10 gives two examples of the blur
metric:
Figure 10 Two examples of the blur metric
The blur population reports the population of panels that have a blur
value above a given threshold. A larger average size can be caused by
a small number of panels with large outlier values or a large number
of panels with a large size. The two values taken together can
indicate whether there is a localized problem over the whole sample
(larger blur value but no outliers), a regional problem (an outlier
population that spans channels), or a transient problem (outliers in a
single channel).
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