Applied Biosystems SOLiD™ 4 System SETS Software User Guide ...

Determine the optimal titration point
Applied Biosystems SOLiD 4 System SETS Software User Guide
Chapter 4 View Reports
View overall run reports 4
Determine which titration has the highest titration metric. This
titration is the optimal titration point.
Determine the bead deposition density for a sequencing run
1. Calculate the concentration of P2-positive beads using the
formula below, where X is the volume of templated beads used
for the WFA sample (equivalent to 15 million beads) and P2# is
given by the WFA report:
2. Use the calculated concentration in place of the value
determined by the NanoDrop ® ND-1000 Spectrophotometer for
more accurate deposition densities when preparing slides for
sequencing. It is recommended to use this resulting calculated
bead concentration to determine the volume of beads for
deposition and multiply that volume by 120%:
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