Applied Biosystems SOLiD™ 4 System SETS Software User Guide ...

C
Appendix C Advanced Topic: Data Management
Saving data
Raw image data
Primary analysis results data
Table 2 Image analysis results files
The data should be kept until the full completion of primary analysis
is verified and primary analysis results are archived. After
confirming that the image files are no longer needed, you can delete
these image files to make room for additional sequencing runs.
The image analysis results are stored in “.spch” files, which are
located in:
$sampleResultFolder/colorcalls/CACHE/.
File type File size Comments
.spch 646 GB Should be archived for the lowest-level non-image backup.
An estimate for the disk space needed for one “.spch” file
(per panel) is n*(18*m+17) bytes, where n is the number of
beads, and m is the number of cycles (F3 and R3).
For 50 cycles (1x50) and 300K beads, this yields 275 MB (per
panel).
A full slide has 2357 panels, which means that 646 GB are
required for a full slide (50 bp single tag run).
Table 3 Color call results files
The color call results, including color-space raw reads and QV data,
are located in:
$smpleResultFolder/primary.[17-digit timestamp]/reads/.
File type File size Comments
.csfasta 56 - 80 GB Should be archived.
_QV.qual 112 - 140 GB Should be archived.
.stats < 10 KB Should be archived.
postPrimerSetP 364 - 420 GB Temporary files. They are removed after completion. Located in
rimary.[JobID]
$sampleJobFolder/.
158 Applied Biosystems SOLiD 4 System SETS Software User Guide