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Applied Biosystems SOLiD™ 4 System SETS Software User Guide ...

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Set up Multiplexing run in ICS<br />

Create a Fragment<br />

and Paired-End<br />

Multiplexing run in<br />

ICS<br />

Assign Libraries in<br />

ICS<br />

<strong>Applied</strong> <strong>Biosystems</strong> SOLiD 4 <strong>System</strong> <strong>SETS</strong> <strong>Software</strong> <strong>User</strong> <strong>Guide</strong><br />

Chapter 6 Support Multiplexing<br />

Set up Multiplexing run in ICS 6<br />

There are two ways to set up a multiplexing run in the ICS:<br />

Creating a multiplexing run using the Run Wizard<br />

Importing a multiplexing run<br />

Note: Refer to the <strong>Applied</strong> <strong>Biosystems</strong> SOLiD 4 <strong>System</strong> ICS<br />

<strong>Software</strong> Help or <strong>Applied</strong> <strong>Biosystems</strong> SOLiD 4 <strong>System</strong><br />

Instrument Operation <strong>Guide</strong> (PN 4448379) for detailed<br />

instructions on these procedures.<br />

Continuing in the Run Wizard, you manually configure each barcode<br />

library to generate a new run definition. You need to save the run<br />

definition before you assign the run to one of the two flowcells.<br />

Using the Run Wizard, assign libraries to the appropriate barcode<br />

from the appropriate multiplexing series by creating a run definition<br />

file.<br />

Note: Refer to the <strong>Applied</strong> <strong>Biosystems</strong> SOLiD 4 <strong>System</strong> ICS<br />

<strong>Software</strong> Help or <strong>Applied</strong> <strong>Biosystems</strong> SOLiD 4 <strong>System</strong> Library<br />

Preparation <strong>Guide</strong> (4445673) for detailed instructions on these<br />

procedures.<br />

107

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