pasteurização de salsichas com ultrassom e micro-ondas - UFSM
pasteurização de salsichas com ultrassom e micro-ondas - UFSM
pasteurização de salsichas com ultrassom e micro-ondas - UFSM
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ABSTRACT<br />
Master Dissertation<br />
Graduate Program in Food Science and Technology<br />
Fe<strong>de</strong>ral University of Santa Maria<br />
SAUSAGE PASTEURIZATION WITH THE USE OF ULTRASOUND AND MICROWAVE<br />
AUTHOR: CRISTINE RAMPELOTTO.<br />
ADVISOR: NELCINDO NASCIMENTO TERRA.<br />
CO-ADVISOR: ALEXANDRE JOSÉ CICHOSKI.<br />
Date and Defense place: Santa Maria, april 12, 2012.<br />
This study aimed to <strong>de</strong>velop new methods for pasteurization of sausages with the use of ultrasound and<br />
<strong>micro</strong>waves in or<strong>de</strong>r to reduce the bacterial load with more energy efficient and low impact on the<br />
characteristics of the product. For pasteurization with <strong>micro</strong>wave oven, sausages were divi<strong>de</strong>d into 22<br />
groups, of which one was the control that not received treatment, and the others received treatment in a<br />
<strong>micro</strong>wave in different conditions, varying the time and power. The effect of the<br />
<strong>micro</strong>wave treated sausage was evaluated with and without a water bath, in the presence or absence<br />
of packaging. Sausages treated with <strong>micro</strong>wave unwrapped in conditions that do<br />
not change visually, reduced mesophilic and acid lactic bacteria statistically significant, but at a rate not<br />
significant for practical use. When treated were packaged, it was observed that the packaging <strong>com</strong>monly<br />
used in sausage has little resistance to <strong>micro</strong>wave pasteurization, and breaks observed in some cases. It was<br />
necessary to use a water bath to maintain maintain package integrity. The data obtained on<br />
the sausage pasteurization with <strong>micro</strong>wave suggest that are required the <strong>de</strong>velopment of suitable<br />
packaging and adapting the machine to produce more uniform heating and controlled.<br />
For pasteurization with ultrasound were analyzed the effectiveness of the frequencies of 25 kHz and<br />
130 kHz in the reduction of mesophilic bacteria and acid lactic bacteria and the effect of frequency on<br />
color, lipid oxidation and pH. We evaluated the potential bactericidal effect of ultrasound of 25 kHz on<br />
pathogenic bacteria Staphylococcus aureus and Bacillus cereus and the effect of storage on<br />
the <strong>micro</strong>biological aspects, lipid oxidation, color, pH and texture of pasteurized sausages with ultrasound.<br />
The frequency of 25 kHz, was more effective in reducing mesophilic bacteria and acid lactic bacteria<br />
and not changing color and pH, as well as producing lower lipid oxidation. The ultrasound effect was<br />
observed on the <strong>de</strong>struction of Bacillus cereus and apparently did not affect the cells of<br />
Staphylococcus aureus. For the avaluated storage conditions, ultrasound promoted inhibition of acid lactic,<br />
psychrotrophic and mesophilic bacteria and lower lipid oxidation up to 60 days of storage, with<br />
minimal color and pH change, without affecting the texture.<br />
Keywords: cooked sausage, pasteurization, <strong>micro</strong>biology, TBARS.<br />
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