Clw@terizclon of tL*Mi. ,1cid in Di&tu',t Pho@dicd Dotuge Fw.d tn ...

iii High pre$we liquid chtohatogaph, (HPLC)
Nmb€r of nelho& for th. dermimtion ol ednziG h6 b.cn rcpon.d 6ing HPLC
tehniquc, Thee nehods dd dEn .l.lails e pEsr€d in rabl€ l. L
HPLC Mhods o!@tihire
HyprsilBDS C0(5 A25 cn x 3.9
mn)@lumn, isriic n d. H,O:
M.CN:Er,N (61:r7r02) DH 7.t.
lnensil-C3 (250 x 4.6 mm) 5 FB
dislill.d Mb, .c.loniril. ud
l.idny|lnin (a):40:.0.|) pl{ - 3.5
CtrNovpak@lunn{l50n9nm:
,tFn) tn6y'Enc & elutid
:.enibiL (6l:39) pn 2.8
Novi-P.l{ Ctrcolumi O50 x 39m,
4lrn) M.CN 0.02 nolLr $dim
d jhydsSo rldphd.$dryLnin
Nov!-Pot Cl8 4 m Edial
@mDE$io oluh|l 5()91 M.CN-
5eZ phosph& buF6 (pH 1.07) lo
which 0.011 sol Ll EtlN
(50:t0,0.15)edO.ol05 noll I
s.dium dodeyl sdfo@ r.E !ddd.
HIT€6il BDS C I3, 5 nu column
i4.5 x 250 mml mobile pl@. of 0.05
M dihydrcgd Dholphd.: @di'ile:
hernmol t.hhydrcftnn (12:t:2: l,
v/v/v/v) .. . pH of 5.5
I-icht@tb Rl-18 colub4 KHTPO.
M.CN (55:!5) {v&)
Lich@|b cr colmi. M9CN 4.01
moll | 3€diuD hydbgen phoehde.
002 moll edium h.mlulfo@
Sfiin p.cl CLC-ODS @hdn (250x
4.6 nm), MeCN - 0.01 M KHrmr
(46:54, tunbiiin! 0.02 M N.
hept n6ulfon'., pH 6.1)
sDhdi$rb 5 OD92 olmn (5 mM
aqt rn buryl @onium phosPn{o -
nclonol kraa hydb lu@(33:65:2
Th. irn@e of rnp.|@. .!d El{iv. hrmidiry on rbbiliry of dirizirc dihydrcchbn{b jn etid
slale wa follos.d bt a HPLC melhod in on€ ofth€ sludy [,t9].
12
,tl
,18
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