Clw@terizclon of tL*Mi. ,1cid in Di&tu',t Pho@dicd Dotuge Fw.d tn ...

3 Dmg- dru! i .ncriob srndi6
The D v,r4 intedction srudies of celirizine sd rll tne o-adninisLrcd dlu8s were cdied out
ar dilfeEnl buffq medims a enpv srom&h (pH l), tull slommh (pH 4), sinulared blood
(!H ?.4) md simdaicd intesdnal juice (!H 9). For rhis purpo$ I oo rsnl r lrock solnlions of
aw t r€ial or.etjrizi& .nd oltq &ues (individlrlly) vw pr@d.d and nixed b rEke
fiml orc.nt?lion oa 5olr8bl-'' fd @h @nnnu.r Th.* l,4 kcpt on ulcr barh for 120
ninules at lfc. Ar dliquol of 5 DI ws qithdsu a116 every 15 minur.s im€ryals ed an€r
n kile suitable dilurions wft $med on Uv-visible specirophotomcid. ln the sme
mamr for sludying inrenctio.s on HPLC, ih. supl.s w@ filt€r€d through 0,45 Fn fill€r
paper anr mrtirg .ppopriat€ diltjoB rd thE. rsPlicals we injcrad in to the srsrem.
Th. co@Fation of @h drg rc d.lemined dd % @vdy itr @h c& w6 c.lculal€d.
4 Dng {.t!l i.rcnctio! rrldid
l,rr'to availability ofc.ririzine in pEerce of n llh ws detemin d by disslvinS l0 ne of
c.t izine in 100 mL mixtue of90:10 n€thsol ed wat r. enicated for l0 Fioul$. Sbct
elulion of th€ inmai sian.hrd, pmpyl peab.n, ws sihilely p@psed. 'nF @e.nl.ation of
bolh sto.k $tunons ws .quiEtot ro 100 |r8DL'. Slek elurioE of n rab srlc
(M8cb.6HtO, caclr.2Hro, crc|i6Hro, M'CD.HrO, F.Cl3.6HrO, CoClr.6HrO, Nicl!6tl,o.
cuclz.2H?o. zich ald CdCl:.Hro) w prcpscd by dislvins 5De ofech in 100 mL of
de-ionizd water ro gilc @ocntradoc of 50 FgoLr.
Equivolme slock elutioB of Delal ud @ti.izine wrc nixed togedt r ro give final
@dcentdion of 50 Fgmlr of edrizine &d 25 lrgnrr of m.tsl salts for dng-neral
int@tior Th6e elurioB wrc pl&€r! in wdd.ba$ nainbin€d ar l1a 5"C for d hou, rh.n
lillcEd lbrcugh a 0.45 rrn lihlr @pe ed injered for 4al'sis.
G SD..rtuopt ltudla oforpld.r
Cdirizire md its conpld$ weE chlEredzd by FT-IR sp€lrophobD.u in the Egion of
409000 qr The infftd rpetr! w@ @rdcd s a Dorasim brenid€ dis nellod.
Polon NMR sludics wF cdied our on a Brukd i$nrnent in deutcrared wrr. m€tnmol
ed cbloofom uin8 l MS .s d inleml slsdrd.