Clw@terizclon of tL*Mi. ,1cid in Di&tu',t Pho@dicd Dotuge Fw.d tn ...

Btf.t pH 7.a (pb''phare btt t)
0.6 g of poi.siM dihydro8.D orlhophosphrtc, 6,4 g of di$dim hydrog€n orthophosphale
&d 5.85 g ofedim chlorid. we disslved iD suffici€nl d€-iooized watd 10 prcducc 1000
rrl- dd th. pH .djusLd if M6sary.
Batet pH E and g(Mm,na bufet)
4.98 g of almonim chloiid. M dis$lv. in l00O triL of deionized water ed pH w4
adjuled qith l0% almonia.
E Uv/viibh.Frt!@pi..ldtlL
I Asat ol c..bbhE shts Vl,-tilttd. sp.ctrophotoadda it tod
P p pa rct io n of s a I ln io h r of c. I tr| z I re
1 DM solulion of @tnizje w pr4olrd by dislvilg 0.0461 g in 100 ol in 3inul.l€d
safic jui@ .Id volMe w m.dc up with thc l.rc elv6r |o lh. @L 25 mL of priony
slution w flnld dilurcd to 250 rI wirh simulated srsiric juice 10 rEl. ih. finrl
con€mt.lion ofo-l bM, rcsFclively, In a sinild way the solutiore w€r€ Flp!rcd
Cohntution of ttlt .otibtution c@t of cetui2itu
in the
Dif&rent dilulioB rmeins liom 0.01 10 0.I nM wcrc pEpsrEd by diluting the $ock slulions
(0.1 nM) vilh difrsert buf* ell'ioN oI pH l -9. Fof rhis purpoe 5, 10, 15, 20, 25, 30, 35,
,lO, 45 .nd 50 6L of slact elidzif slutioo *s eFrr.ly piFn d out in leo diff.Mt 50
nL voldetric llaks &d dilllld *il[ individusl hfftr $lurio6 up ro lbe b!* to F!pd.
rhe solulions of 0.01-0.I mM. Ih. ab6o6dce ruim w@ emqd in th€ Esion of 190-700
m for th.& &lutions agaiNt O. eagent blark. Th€ @ima rlFaFd at 231 M. Gophs
w ploltld for ab6o$rne asaini @nqr.don dd sniish lin @ obl,.iftd $,hich
ob€yed Ber lr$err's l3*. Epiilo valB qe ..lcllll€d n@ tF obsemric.
2 conttra.tlon ofth..atbtutirDc4Molltutuctins.trugs
To pl€p@ calibdrion cunes of int€@lin8 do8!, difreHt lir€&ity dges wrc seledcd dd
@ p@r.d i! laue 2.2. Gnphs 9@ plon d for .bsbs. .gd.n @r..lio. .!d !
sfaieli lie m ohained enich oberd th. BaR |ry.
39