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Effects of reduced plant height on breeding requirements and ...

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Experiment lV: _-.t0qlepptite leneth Stuqy<br />

This experiment was c<strong>on</strong>ducted to determine the relati<strong>on</strong>-<br />

ship between eoleoptite length <strong>and</strong> <str<strong>on</strong>g>plant</str<strong>on</strong>g> <str<strong>on</strong>g>height</str<strong>on</strong>g> in seleeted<br />

taIl <strong>and</strong> semí-dwarf barley genotypes described in Tabte J.<br />

Four semi-dwarf <strong>and</strong> three tall genotln)es were lncluded.<br />

The study was c<strong>on</strong>ducted at the Universíty <str<strong>on</strong>g>of</str<strong>on</strong>g> Minnesota,<br />

U.S.A. in February, 1976. A four replicate c<strong>on</strong>pletely<br />

r<strong>and</strong>omized block design was used, each replicate c<strong>on</strong>sisting<br />

<str<strong>on</strong>g>of</str<strong>on</strong>g> six seeds <str<strong>on</strong>g>of</str<strong>on</strong>g> each genotype individually pi.aced embryo<br />

upright ín the center <str<strong>on</strong>g>of</str<strong>on</strong>g> a paper towel" This towel was<br />

then rolled up, fastened, placed in a beaker c<strong>on</strong>taining<br />

water <strong>and</strong> placed ín a dark incubator for germinati<strong>on</strong>. Síx<br />

seeds were used to insure a sarnple size <str<strong>on</strong>g>of</str<strong>on</strong>g> five for each<br />

replicate <str<strong>on</strong>g>of</str<strong>on</strong>g> each genotype" The incubator was kept at 15<br />

degrees C for seven days, but due to this temperature, germinati<strong>on</strong><br />

did not ensue <strong>and</strong> the temperature was raised to 2j<br />

deg"ees C for seven more days. After this, the material was<br />

removed <strong>and</strong> the following measurenents were taken:<br />

(1) Coleoptile ]-ength (mm.).<br />

(2) Seminal root number"<br />

(3) Root score - based <strong>on</strong> a visual rating in which<br />

roots were scored as l-<strong>on</strong>g, medium, or short. lhese<br />

were given weights <str<strong>on</strong>g>of</str<strong>on</strong>g> three, two <strong>and</strong> <strong>on</strong>e respectively,<br />

with the sum c<strong>on</strong>stituting the total- score.<br />

( 4) sfroot length ( rnm. ) .<br />

Analyses <str<strong>on</strong>g>of</str<strong>on</strong>g> variance were computed to determine dif-<br />

51

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