2011 (SBTE) 25th Annual Meeting Proceedings - International ...

C.E. Ambrósio
mbrósio, C.V. Wenc
enceslau
eslau, J.L. Nogueir
gueira,
et al. 2011. Células-tronco de membranas fetais para aplicações em
pequenos animais. Acta Scientiae Veterinariae. 39(Suppl 1): s97 - s101.
Acta Scientiae Veterinariae, 2011. 39(Suppl 1): s97 - s101.
ISSN 1679-9216 (Online)
Fetal membranes stem cells application in pets
Carlos Eduardo Ambrósio 1 , Cristiane Valverde Wenceslau 2 , José Luiz Nogueira 4 , Dilayla Kelly de
Abr
breu
4 , Elaine Apar
parecida Fer
ernandes Rodr
drigues
4 , Thais Bor
orges Lessa
4 , Daniele dos San
antos Mar
artins
1 ,
Luciana Relly Bertolini 3 & Maria Angelica Miglino 4
ABSTRACT
Background:Stem cells are precursor cells that have the capacity for self-renewal and could generate cells with characteristics
similar to cells and differentiation, generating varied cell lines. Considering the plasticity of cells can be classified into
totipotent, pluripotent or multipotent.According to the isolation period, the stem cells can be classified as embryonic, fetal and
adult. In the embryo stage are considered totipotent because they can rebuild any tissue in the body and adulthood are
considered multipotent, since they have a more limited plasticity. The fetal tissues and the fetus is a potential source for stem
cells, since they expand more rapidly compared to the cells after birth. Stem cells of fetal membranes are derived from extraembryonic
tissues with high capacity to differentiate into various tissues. The cord blood stem cells have mesenchymal and
hematopoietic, and mesenchymal cells have the potential to proliferate and differentiate into multiple cell lineages. The yolk
sac in dogs is morphologically composed of three layers: a single layer of endoderm, a simple mesothelium, and intermediate
to them, the vascular mesenchyme. Work identified a population of pluripotent cells in the yolk sac can differentiate into
hematopoietic cells, however, can be isolated mesenchymal stem cells. In this review we aim to focus new isolations of cells
from umbilical cord blood and yolk sac of dogs, reviewing the main literature on this species. The importance of using dogs
out of work has intensified in recent years, since many diseases can manifest itself in a similar way to humans. Additionally, the
dog is a pet, and interest in the treatment of diseases and improved quality of life of this species has been accentuated in
veterinary medicine. Thus, identifying the cellular sources in the dog opens new horizons for preclinical studies and new N
therapies for veterinary medicine.
Review: This study is related to morphological biology multipotent stem cells, focusing its expansion and use in cell therapy
in animal models that have different pathologies. A widely studied model for muscular dystrophy is the GRMD (Golden
Retriever Muscular Dystrophy), which is homologous to DMD (Duchenne Muscular Dystrophy) that affects humans. It is a
recessive genetic disease, X chromosome which affects approximately 1 in every 3500 boys. It is characterized by a progressive
muscle degeneration, resulting from the absence or reduction in the production of dystrophin protein present in the sarcoplasmic
membrane of muscle fibers.
Conclusion: The use of cells derived from fetal tissue are strong candidates for veterinary regenerative medicine, since they
have high capacity for cellular differentiation. The use of fetuses and fetal tissues of humans still has limitations, so the dog is
a viable alternative for studies of fetal stem cells. Thus, it is extremely important to know the characteristics of morphology and
proliferation of cells derived from fetuses and fetal annexes canines, including yolk sac and umbilical cord as well as know the
feasibility of clinical application of these cells in preclinical testing in animal models and eventually in human medicine, thus
contributing to regenerative medicine.
Keywords: stem cells, fetal membranes, dogs, therapy.
CORRESPONDÊNCIA: C.E Ambrósio [ceambrosio@usp.br- Fone: + 55 (11) 3565-4112]. Faculdade de Zootecnia e Engenharia de Alimentos
(FZEA), USP. Av. Duque de Caxias Norte n. 225, ZAB. CEP 13635-900, Pirassununga, SP, Brazil. 1 Faculdade de Zootecnia e Engenharia
de Alimentos (FZEA), Universidade de São Paulo (USP)-Pirassununga, SP, Brazil. 2 Instituto Butantan, São Paulo, SP, Brazil. 3 Universidade de
Fortaleza (UNIFOR), Fortaleza, CE, Brazil. 4 Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, SP, Brazil.
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