2011 (SBTE) 25th Annual Meeting Proceedings - International ...

Acta Scientiae Veterinariae, 2011. 39(Suppl 1): Abstracts - 25th Annual Meeting SBTE-Brazil. August 2011.
A029 MALE REPRODUCTIVE PHYSIOLOGY AND SEMEN TECHNOLOGY
EFFECTS OF SELENIUM AND CHROMIUM ON SEMINAL QUALIT
ALITY OF BUFF
UFFAL
ALOES SUPPLEMENTED WITH BYPRODUCT
ODUCTS OF
AMAZONIAN AGROINDUSTRY
Alessandra Ximenes Santos 1 , Alexandre Rossetto Garcia 2 , Cristian Faturi 3 , Benjamim De Souza Nahúm 4 , José Brito Lourenço Junior 5 , Sâmia Rubielle Silva De
Castro 6 , Geanne Rocha Silva 7 & Arnaldo Algaranhar Gonçalves 8
1,7,8
UNIVERSIDADE FEDERAL DO PARÁ, BELEM, PA, BRAZIL. 2,4 EMBRAPA AMAZÔNIA ORIENTAL, BELÉM, PA, BRAZIL. 3 UNIVERSIDADE FEDERAL RURAL DA AMAZÔNIA, BELÉM, PA,
BRAZIL. 5 UNIVERSIDADE DO ESTADO DO PARÁ, BELÉM, PA, BRAZIL. 6 FACULDADES INTEGRADAS DO TAPAJÓS, SANTARÉM, PB, BRAZIL.
The use of organic industrial byproducts available on Amazon, such as coconut meal (CM) and palm kernel cake (PKC), may
constitute an bioeconomic alternative to replace conventional concentrates for ruminants, leading to environmental and productive gains.
Therefore, the aim was to evaluate the semen quality of buffalo supplemented daily with experimental diets based on PKC or CM, with different
levels of selenium (Se) and chromium (Cr) intake. Fifteen buffaloes (3.2±1.8 years, 578.6±101.9 kg) raised on pasture (Panicum maximum) at
the Embrapa Eastern Amazon were daily supplemented with isoproteic concentrates (1% BW) during 252 days. Animals had similar initial
semen quality and were divided into three groups: Control (5 bulls; conventional ration with 62% corn grain), T1 (5 bulls; ration with 69.3%
CM) and T2 (5 bulls; ration with 69.3% of PKC). Bulls had semen collected weekly, starting 112 days after first day of supplementation and two
complete spermatogenic cycles, according to Sharma and Gupta (1980, Animal Reproduction Science, 3, 217-224). The ejaculates (n = 173)
were evaluated for sperm motility, integrity of plasma membrane and sperm morphology. The level of Se and Cr in the feedstuffs was determined
according to EPA-Method 7742/6010 (2009, U.S. Environmental Protection Agency, 26p). Data were subjected to ANOVA, with comparison
by t test and the association between variables was analyzed by Pearson correlation (P < 0.05). The average of daily intake of Se (mg/day) for
Control, T1 and T2 was 1.37±0.35 B , 1.15±0.26 C and 2.80±0.66 A (P < 0.05), respectively. Daily consumption of Cr (mg/day) in Control, T1 and
T2 was 7.17±1.87 B , 4.30±0.97 C and 20.75±4.94 A (P < 0.05), respectively. Sperm motility was significantly higher (P < 0.05) in T2 (71.7±15.1% A )
compared to Control (59.3±20.5% B ) and T1 (56.7±24 8% B ). Higher level of plasma membrane integrity (P < 0.05) was also observed in T2
(82.1±12.2% A ) compared to T1 (72.0±22.6% B ) and Control (69.2±19.4% B ). There was no significant difference in the sperm total defects
(Control: 44.2±18.5%, T1: 41.3±16.1% and T2: 44.3±19.2%). Correlation between consumption of Se and Cr with sperm motility (r=0.36 and
r=0.34, P < 0.0001) and with plasma membrane integrity (r=0.33 and r=0.32, P < 0.0001) were significant and of medium intensity. Thus it can
be concluded that selenium and chromium presented positive effects on preservation of plasma membrane integrity and on sperm motility, but
had no interference on cellular morphology.
[Acknowledgments. Project “Network for Innovation in Animal Reproduction” (01.07.01.002)].
Keywords: semen, micronutrients, bubalus bubalis.
A030 MALE REPRODUCTIVE PHYSIOLOGY AND SEMEN TECHNOLOGY
EFFICIENCY OF THE CAP
APACIT
CITATING TING MEDIA ON MOTILIT
TILITY MAINTENANCE AND INDUCTION OF EQUINE
SPERMATOZ
OZOA HYPERACTIV
CTIVATION
TION
Daniela Franco da Silva, Rubens Paes de Arruda, Thayna Pantoja Gardes, Rafaela Nogueira Rodrigues Cardoso, Juliana
Nascimento, Henrique Fulaneti Carvalho, Kleber Menegon Lemes & André Furugen Cesar de Andrade
N
USP, PIRASSUNUNGA, SP, BRAZIL.
Hyperactivation is characterized by a non-progressive sperm trajectory and an overt lateral displacement of the head, which is
essential for the occurrence of penetration of the zona pellucida (Suarez, 2008; Human Reproduction Update, 14 (6), 647-57). The aim of this
study was to test by CASA, the capacitating media F (Andrade et al., 2008; Animal Reproduction Science, 107, 304-5) and the capacitating media
M (MCPARTLIN et al., 2008; Theriogenology, 69, 639-50) in equine spermatozoa, in order to determine which was most efficient in promoting
hyperactivation after 5 h of incubation. Three ejaculates from three stallions was collected and cryopreserved (n = 9). The semen was thawed,
selected in Percoll ® and tested in the two media. The motion characteristics analyzed by CASA were total motility (MT%) and progressive
motility (MP%) after 0, 30, 60, 120 and 300 min of incubation at 38°C and 5% CO2. In addition, there were adjustments of the tool Edit / Sort
to assess the percentage of hyperactivated cells. The equine sperm was considered as hyperactivated when VCL> 180 µm and ALH > 12 µm
(Rathi et al., 2001; Biology of Reproduction 65, 462-470). The data were analyzed using ANOVA and Tukey test and are presented as mean and
standard deviation. The mean value of MT in medium F was 64.23±19.22%; 58.9±21.74%; 49.38±13.21%; 47.23±15.44% and 28.44±10.22%
in time 0, 30, 60, 120 300 respectively. The medium M mean value was 62.43±21.19%; 52.94±22.90%; 49.8±18.8%; 43.46±17.94% e
22.51±11.41%. The MP values of medium F in time 0, 30, 60, 120 e 300 min were 50.17±15.81%; 45.27±17.78%; 39.20±9.93%; 37.91±13.41%;
24.37±9.92%, respectively. The MP mean values of the M medium was 48.87±18.30; 43.18±19.35%; 41.52±16.26%; 36.20±15.62%;
19.61±9.11%. There was no interaction between treatment and incubation time regarding to sperm hyperactivity variable and the results was
higher in medium F 8.48±4.38%; 7.66±4.92%; 7.68±6.73; 6.86±4.65% e 4.84±4.52% when it was compared with the mean values of medium
M 6.85±1.41%; 6.00±2.83%; 5.11±2.21%; 4.39±3.19% e 2.35±2.40%. There were no differences between the two tested medium. Although
both medium was considered efficient in maintaining sperm motion characteristics after 300 min of incubation, the F medium was better in
inducing hyperactivation of sperm cells. [We thank Fundação de Amparo à pesquisa do Estado de São Paulo – FAPESP (Process: 09/54906-
5, 09/50474-3, 10/01912-5, 10/01916-0). We thank Botu-Pharma that kindly donated the cryoprotectant for this experiment].
Keywords: capacity, hyperactivation, spermatozoa.
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