2011 (SBTE) 25th Annual Meeting Proceedings - International ...

Acta Scientiae Veterinariae, 2011. 39(Suppl 1): Abstracts - 25th Annual Meeting SBTE-Brazil. August 2011.
A045 FOLLICULOGENESIS, OOGENESIS AND SUPEROVULATION
COMP
OMPARISON BETWEEN LUTEAL PHASE PERIOD AND THE NUMBER OF FOLLICULAR
WAVES IN BOS TAUR
URUS
US,
BOS INDICUS AND BUB
UBAL
ALUS BUB
UBALIS
HEIFERS MAINTAINED AINED ON THE SAME NUTRITIONAL AND
ENVIRONMENT
ONMENTAL STATUS
TUS
Julia Maria Baldrighi, Manoel Francisco Sá Filho, José Antonio Visintin , Pietro Sampaio Baruselli & Mayra Elena Assumpção
UNIVERSIDADE DE SÃO PAULO, SÃO PAULO, SP, BRAZIL.
Bovine luteal concentration after ovulation maintains the estrous cycle until luteolysis, when fertilization does not occur. During this
cycle, corpus luteum (CL) development shows three phases: growth, static and regression. Thus, the luteal period involves the first two phases,
when the CL is active. Considering these facts, an hypothesis is that the greater the luteal phase, the higher the number of follicular waves in one
estrous cycle. The main objective of this study was to compare the luteal phase period with the number of follicular waves in one estrous cycle
from Bos taurus (Holstein; n = 14), Bos indicus (Gir; n = 5) and Bubalus bubalis (Murrah; n = 15) heifers under the same nutritional and
environmental conditions to avoid variations due to time and management influences. All 34 heifers were housed in pasture at the Department
of Animal Reproduction, College of Veterinary Medicine, University of Sao Paulo, at Pirassununga Campus and they were synchronized with
two doses of prostaglandin F2α (PGF; D-cloprostenol, 150 µg IM) fourteen days apart. From D0 (D0 = day of first ovulation), ultrasound scan
of the ovaries was performed daily to follow their follicular dynamics (follicles = 3mm) and to record number of follicular waves, CL measures
during inter-ovulatory interval (IOI) (duration of luteal phase) and the duration of the IOI in one estrous cycle. No significant difference was
found between the IOI (22.2±0.65; 22.1±0.95; 24.0±0.65 days) for the Holstein, Gir and Buffalo, respectively. On the other hand, the number
of follicular waves in one estrous cycle (2.8±0.15a, 3.4±0.24b, 2.8±0.13a waves; P < 0.05) and the duration of luteal phase (13.4±2.4a,
16.0±0.0b, 15.4±0.74b days; P < 0.003) were statistically different for the three breed, Holstein, Gir and Murrah, respectively. Although there
were no differences found the IOI among the three breeds, the zebu heifers showed higher number of follicular waves (60% with 3 waves and
40% with 4 waves) and greater luteal phase than the other two breeds. After evaluating the three breeds at the same nutritional and environmental
status it is possible to conclude that the CL activity may be involved in the number of follicular waves during one estrous cycle in Bos taurus,
Bos indicus and Bubalus bubalis heifers.
Keywords: ultrasound, corpus luteum, estral cycl.
A046 FOLLICULOGENESIS, OOGENESIS AND SUPEROVULATION
BEHAVIOR OF A NEW POLYMORPHISM ON GDF-9 GENE (FECGSI) IN EWES OF SANTA A INÊS BREED IN FRONT OF
SUPEROVUL
OVULATION PROTOC
OCOLS
OLS
Bianca Damiani Marques Silva 1 , Thiago Antonio de Souza Nascimento Silva 2 & Jairo Pereira Neves 3
1
EMBRAPA, ASA NORTE, DF, BRAZIL. 2,3 UNB, ASA NORTE, DF, BRAZIL.
Many polymorphisms were described in GDF-9 and BMP-15 genes, but only one showed high ovulation and prolificacy in
homozygosis without sterility. The aim of this study was evaluated the superovulatory answer and embryo yield on FecGSI polymorphism ewes
in two superovulation protocols. Material and Methods Superovulation e embryo recovery was made in 18 Santa Inês ewes (6 E/E (mutated
homozygote), 6 +/E (heterozygote) e 6 +/+ (wild homozygote)) to compare ovarian response among genotype. The ewes were randomly
distributed in two protocols of superovulation: traditional protocol and Day 0 protocol. Each treatment was repeated twice in a cross-over model
intercalated of two months each. Was used fresh semen for AI of one male with proved fertility. Five days after AI the embryos were recovered
cirurgically, by laparotomy. The statistical analyses were factorial 3x2 with variance analyses by SAEG 9.1. Results There was no difference
among evaluated parameters (P > 0.05) between Day 0 protocol (total CL 9.8 ± 5.3; total structures 4.5 ± 4.6; viable structures 1.6 ± 2.0) and
traditional protocol (total CL 10.0 ± 6.0; total structures 3.5 ± 4.3; viable structures 1.7 ± 2.4), probably because of precocious acquisition of LH
receptors changing the follicular dominance process. Corpora lutea in the ovaries (E/E 9.0±6.3; +/E 10.1 ±5.3; +/+ 10.5±5.3), total structure
recovered (E/E 4.9±5.0; +/E 3.1±3.1; +/+ 4.1±5.2), and viable embryos (E/E 1.9±2.1; +/E 2.2±2.6; +/+ 0.9±1.7), there was no difference (P >
0.05) among groups of genotype animals. Was observed higher number of animals answering the superovulation treatment when used Day 0
protocol (10/18) in comparison with traditional protocol (6/18). The ewes that answered to superovulation protocol, regardless the protocol, by
genotype, there were no difference E/E 6/12; +/E 5/12 e +/+ 5/12. Was not observed alteration in ovaries, in follicular growth and neither in viable
embryo production when compare among genotypes. Conclusions Both protocols were efficient in superovulation and embryo production, but
there were no difference among evaluated genotypes in ovulation rate, possibly for any alteration in FSH receptor, influenced by the new
genotype or by the forward fase of FSH application.
Keywords: ovulation rate, embryo, polymorphism.
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