2011 (SBTE) 25th Annual Meeting Proceedings - International ...

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Acta Scientiae Veterinariae, 2011. 39(Suppl 1): Abstracts - 25th Annual Meeting SBTE-Brazil. August 2011. A103 FTAI, FTET AND AI INFLUENCY OF THE UTERINE LAVAGE TO ENDOMETRIAL CYTOL OLOGY OGY ON THE BOVINE FERTILIT TILITY Helder Esteves Thomé, Rubens Paes De Arruda, Bruna Marcele Martins De Oliveira, Milton Maturana Filho, Guilherme Cain De Oliveira, Shirley Andrea Rodrigues Florez, Carina De Fátima Guimaraes & Eneiva Carla Carvalho Celeghini DEPARTAMENTO DE REPRODUCAO ANIMAL - FMVZ - USP, SAO PAULO, SP, BRAZIL. Uterine defense mechanisms against environmental microorganisms include anatomical and functional barriers, as non-specific immune responses (polymorphonuclear inflammatory cells) and specific (humoral antibodies). This rapid and intense influx of inflammatory cells at the site of semen deposition, after the artificial insemination (AI), is described as “post-breeding inflammatory response” and can be intensified with the deposition of a higher number of dead sperm during AI and delay the uterine clearance, featuring a subclinical endometritis, which may develop into clinical endometritis. The diagnosis of subclinical endometritis have been described by several techniques, being characterized by the proportion of polymorphonuclear cells in a cytological sample taken from the uterus of clinically healthy cows, through uterine biopsy, cytobrush or cotton swab and uterine lavage. However, these methods are invasive and can influence the fertilization rate of these animals. Given this, it was aimed to evaluate the conception rate in cows submitted to uterine lavage four hours after the AI. Have been used 127 Nellore cows, lactating, undergoing a protocol of timed artificial insemination (TAI), which consisted of inserting a subcutaneous implant containing 3 mg of norgestomet (Crestar ® ) plus the IM application of 2 mg of estradiol benzoate (Estrogin ® ), eight days after the progestin implant was removed, administrating 1 mg of estradiol benzoate, 0.5 mg of sodium cloprostenol (Sincrocio ® ), and 300 IU of equine chorionic gonadotropin (Novormon ® ), performing a TAI 36 h later. The uterine lavage was performed in 35 cows, four hours after AI by the intrauterine infusion of 40 mL of sodium chloride solution 0.9%, keeping it for 10 seconds and then the liquid was removed. Pregnancy diagnosis was performed 35 days after TAI. The qui-square analysis was used to determine the frequency of the pregnancy rate by PROC FREQ of SAS, version 9.2 (SAS, 2010). From the animals that went through uterine lavage, was obtained a pregnancy rate of 54.28% (19/35) against the rate of 55.43% (51/92) of the animals that had not manipulated its uterine environment, being observed no difference (P > 0.05) in pregnancy rate between the group which was submitted to uterine lavage and that which was not. In this way, it can be concluded that the uterine lavage performed four hours after AI for uterine cytological evaluation does not influence fertility in cows. [Acknowledgment: FAPESP, process number 09/50365-0]. Keywords: pathology, cow, endometritis. A104 FTAI, FTET AND AI ARTIFICIAL INSEMINATION IN PHEASANTS S LODGED IN INDIVIDUAL CAGES Osvaldo Almeida Resende 1 , Jaci De Almeida 1 & Edward Pompeu Ponte 2 1,2 UBM - CENTRO UNIVERSITARIO DE BARRA MANSA, BARRA MANSA, RJ, BRAZIL. 3 SITIO DAS AVES, MAGÉ, RJ, BRAZIL. The exploration of ornamental birds has been being practiced for the birds and feathers commercialization, in small farms in the Brazil. However the handling of natural matings (NM) of these birds, in individual or collective pens has not been presenting compatible performance with the commercial needs. Thus a venerated pheasants’ reproductive project (40 males and 200 females) using the method of artificial insemination (AI) was developed in a particular poultry farm, located in the Rio de Janeiro’s State, objectifying make possible the commercial exploration of male tails feathers. The pheasants were housed at the 16 age weeks, in individual galvanized wire cages, measuring 100x80x82cm (male) and 25x44x42cm (female), kept in triple arrays, in covered shed, side long wire screened, measuring 30.0x5.0x2.3m The semen collection technique was accomplished by means of abdominal and loin-sacrum massages (Resende et al., 1983, Rev. Bras. Reprod. Anim., 7, 2: 23), twice a week with the males supported on a on a table. The semen was gathered in 0.25mL straw, coupled in insulin syringe plastic. To avoid spermatic losses was put in the straw a drop of lactate ringer solution and afterwards to collect diluted in the same extender, in proportion considering the volume and the concentration of the ejaculate. The artificial inseminations were made in oviduct twice a week, after 14:00hs, in the dose of 0.02mL of the diluted semen, using straw of 0.25mL coupled to the plastic insulin syringe, with the female supported on top of the cage. The eggs were kept by 7 days, in cold chamber (12-15 o C). The incubations were done in small incubators electric, with electronic adjustment of humidity, temperature and turns of eggs, according to the technical recommendations for the specie. The evaluated reproductive data were the averages of the seminal characteristics, egg production and fertility embracing the relative activities to periods of 41 to 48 age weeks. The general average results obtained in the evaluated periods were volume of 0.05-0.3mL, motility of 70-90%, vigor of 2-5 (0-5) to the seminal characteristics (selected males), eggs production of 86.8±4.7% and fertility of 94.1±3.2%. Results these best (P < 0,05) to the average values of egg production (61.7±23.8%) and fertility (72.2±16.1%) obtained in the natural mating control groups of the venerated pheasants, managed concomitantly, in 40 collective pens (1male and 2-3 females). The lodging system of pheasants in individual cages of wire and the reproductive method did not affect the bird’s health (breast and foot lesions were not observed). The project using AI showed to be technically viable for pheasants’ reproductive activities lodged in individual cages. Keywords: artificial insemination, pheasants, cages. s388
Acta Scientiae Veterinariae, 2011. 39(Suppl 1): Abstracts - 25th Annual Meeting SBTE-Brazil. August 2011. A105 FTAI, FTET AND AI EARLY EMBRYO MORTALIT ALITY IN BEEF COWS AFTER FIXED TIME ARTIFICIAL INSEMINATION (FTAI) AND HORMONAL TREATMENT MENTS Rui Machado, , Mateus José Sudano, Marco Aurelio Bergamaschi, Guilherme De Paula Nogueira, Claudia Maria Bertan Membrive & Mário Binelli EMBRAPA PECUÁRIA SUDESTE, SAO CARLOS, SP, BRAZIL. Failure in the maternal recognition of pregnancy is implicated with early embryo mortality. This study evaluated strategies to optimize luteal function of cows after FTAI. Forty-five Nelore (Bos taurus indicus) cows received one progesterone (1.9g) intravaginal device (P4D) and 2mg estradiol benzoate. Seven days later received 0.25mg of PGF2α analogue. Two days after that the P4D was removed and 0.5 mg of estradiol cipionate was given. FTAI (D0) took place 48 h after P4D removal. Females were distributed into: Tcont (n = 9) - nothing further; TPGF (n = 10) - 0.25mg of PGF2α analogue on D14, D16 and D18 (negative control group); TGnRH-hCG (n = 14) – 200mcg of gonadorrelin (GnRH) on D5 and 2500 IU of hCG on D12; and TeCG (n = 12) - 400 IU eCG at P4D withdrawal. All drugs but P4D were given IM. Cows were submitted to: plasma progesterone determination [P4] on D14, D16, D18, as well as ultra-sound examination of ovaries and transcervical embryo collection on D18. Concepti were classified according to integrity and length (whole = WH, fragmented = FR or severely fragmented = SF). Results were analyzed through ANOVA or the Chi-square test. Regardless of treatment, recovery rate was 75.5% (34/45) and retrieved concepti were 12 WH, 4 FR and 18 SF. Early embryonic losses were: 40% (2/5) for Tcont; 41.7% (5/12) for TGnRH-hCG ; 25% (2/8) for TeCG and 100% (9/9) for TPGF. The number and the size of CL were smaller (P < 0.05) for TPGF when compared to the other groups. TPGF cows produced only SF concepti and their [P4] were subluteal ( 0.05) between [P4] of Tcont and TeCG (both above 1ng/mL). The [P4] on D18 was subluteal (
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