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Acta Scientiae Veterinariae, 2011. 39(Suppl 1): Abstracts - 25th Annual Meeting SBTE-Brazil. August 2011. A075 FTAI, FTET AND AI FOLLICULAR AND REPRODUCTIVE TRAITS IN SHORT-TERM PROGESTERONE AND PGF2ALPHA BASED ESTROUS- SYNCHRONIZA ONIZATION PROTOC OCOL OL IN SHEEP Rodrigo V Allende, , Fernando R Saravia, Evelyn N Lara, Andrea F Leiva, T Díaz & José Franscisco Cox UNIVERSIDAD DE CONCEPCIÓN, CHILLÁN, CHILE. The conventional protocols to estrous synchronization based on 12-day progestagen treatments and eCG at intravaginal device removal has no particular advantage based on follicular dynamics in sheep (Bartlewski et al., 2011). The aims of this study were to describe size and static phase of preovulatory follicles, interval to ovulation and fertility post AI of ewes subjected to a short-term progesterone treatment. At mid breeding season, 20 Suffolk ewes under controlled feeding receive an intravaginal progesterone-release devise (CIDR G; 0.3 g progesterone, Pfizer) for 7 days combined to cloprostenol (0.125 mg im Estrumate, Intervet) at CIDR removal (n = 10 control group) or the same protocol plus eCG (400 IU Novormon, Syntex) at CIDR removal (n = 10). Antral follicular dynamics was followed by daily transrectal ultrasonography (10- MHz probe; Honda 2010Vet, Japan). Follicles > 2,5 mm and CLs were measured daily and recorded by sketches on ovarian charts. To determine ovulation time, ewes were scanned every 8 h since 12 h after estrus detection until ovulation, and this moment was the middle period between the last identification of preovulatory follicles and their disappearance. The preovulatory follicular diameter was measured on ovulated follicles and the persistency of the static phase was calculated as the interval between they reached 4.0 mm in diameter until ovulation. To assess fertility after treatment, 256 ewes (2.5-3.0 BCS, 1-5 scale) were synchronized by the progesterone treatment plus eCG and were inseminated at estrus with fresh semen. Follicular size and persistency and interval to estrus and ovulation were compared by the Student’s t-test. The results showed that ovulatory follicles from ewes treated with eCG have similar features than those from control group (6.3mm±0,21 vs.. 6.5mm±0,22), similar behavior at persistency (4.47±0.51 vs.. 3.92±0.29 days) and so for ovulatory rate (1.63 vs.. 1.63). However, the intervals between CIDR retrieval and estrus and ovulation were shorter for eCG group than for control (32.8±3.23 vs.. 45.8±2.42 h and 61.9±2.48 vs.. 82.2±4.97 h, P < 0.05). In addition, the corpus luteum diameter size at day 10 was also different (11.7±0.4 vs.. 10.9±0.28 mm for eCG and control respectively; P < 0.05). The preliminary results show that 83.7% ewes were inseminated with a conception rate of 53.2% (checked by ultrasound at 30 day after AI). The cumulative results indicate that a short-term progesterone protocol combined to PGF2a and eCG allows estrous synchronization with normal fertility after AI. [Reference: Bertlewski PM, Baby TE, Giffin GL. 2011, Anim Reprod Sci, 124:259-268. Funded by Consorcio Ovino (Project FIA FIC-CS-C-2006-1-P-001-22)]. Keywords: estrus synchronization, folliculogenesis, sheep. A076 FTAI, FTET AND AI EFFECT OF CIDR ® REUTILIZATION TION IN A PROGESTER OGESTERONE-PGF2ALPHA ONE-PGF2ALPHA BASED ESTROUS-SYNCHR OUS-SYNCHRONIZA ONIZATION PROTOC OCOL OL ON ESTROUS PRESENTATION TION AND OVULATOR ORY PERFORMANCE IN SHEEP Fernando R Saravia 1 , Rodrigo V Allende 2 , Evelyn N Lara 3 , Andrea F Leiva 4 , T Diaz 5 , P Rojas 6 , M Recabarren 7 & José Franscisco Cox 8 1,2,3,4,5,6,7,8 UNIVERDSIDAD DE CONCEPCIÓN, VICENTE MENDEZ 595, CHILE. The use of short-term progesterone (P4) treatments allows the intensive use of P4 vaginal inserts. The study was aimed to describe the P4 profiles and follicular size and ovulation after the use of new and 7-day used inserts in short-term P4 protocols. In Experiment 1, 12 Suffolk ewes under controlled feeding and during anestrus season, were randomly grouped and treated by a new P4-release device (CIDR G ® , Pfizer) for 7 days (n = 6) or a used one (n = 6). Blood was collected to measure plasma P4 by RIA (Coat a Count, DPC, USA). In Experiment 2, 38 Suffolk ewes (2 replicates) were synchronized during the breeding season by a 7-day treatment with new (n = 22) or used CIDR G ® (n = 16) plus cloprostenol (0,125 mg Estrumate ® , Intervet) at CIDR ® retrieval. Half of the ewes in each group received 400 IU of eCG (Novormon ® , Syntex). Estrous detection was carried out thrice a day by teaser rams. The diameters of ovulatory follicles were measured by daily ultrasonography (10-MHz linear array probe; Honda 2100Vet). Since 8 h after estrus, follicles =3.5 mm in diameter were measured each 8 h and sketched on ovarian charts to identify ovulation. Ten day later, ovaries were scanned to identify corpora lutea to confirm ovulation points. The moment of ovulation was defined as the middle point between the last identification of preovulatory follicles and their disappearance. Data were calculated and comparisons were done P4 by the Student’ t test and by Factorial ANOVA. Results in Exp 1 show that P4 profiles differed in ewes treated with new and used CIDR (0.2±0.1 vs.. 0.3±0.07; 5.3±1.48 vs.. 2.1±0.64; 4.7±1.3 vs.. 1.9±0.24; 3.6±0.77 vs.. 1.9±0.25; 2.5±0.26 vs.. 1.5± 0.25; 2.2±0,58 vs.. 1.2±0.16 ng/mL at days 0,1,2,3,5 and 7 for CIDRs new and reutilized respectively; P < 0.05). Results in Exp 2, show that ewes treated with new and used CIDR have similar reproductive response (P < 0.10), therefore data were grouped and used to assess the effect of eCG in the 7-day P4 protocol. eCG has no effect on ovulatory follicle diameter nor on ovulatory rate or number of follicles ovulated (6.2±0.14 vs.. 6.2±0.2 mm; 100% (22/22) vs.. 100% 16/16); 1.69 (37/22) vs.. 1.69 (27/ 16) for eCG and untreated controls respectively; P > 0.10). However eCG increases the diameter of CL and reduces the intervals to estrus and ovulation (11.3±0.3 vs.. 10.7±0.2 mm; 32.0±1.78 vs.. 45.5±1.71 h; 61.2±1.49 vs.. 80.7±2.81 h for eCG and control groups respectively; P < 0.05). The reduced interval to ovulation and the increase in CL diameters suggests that eCG increases the estradiol production of ovulatory follicles in short-term P4 protocols. [Funded by Consorcio Ovino (FIA FIC-CS-C-2006-1-P-001-22)]. Keywords: estrous synchronization, ovulation, sheep. s374
Acta Scientiae Veterinariae, 2011. 39(Suppl 1): Abstracts - 25th Annual Meeting SBTE-Brazil. August 2011. A077 FTAI, FTET AND AI EFFECT OF ESTRADIOL BENZOATE AT CIDR INSERTION ON OVARIAN TRAIT AITS AFTER SHORT-TERM PROGESTER OGESTERONE ONE AND PGF2ALPHA BASED ESTROUS-SYNCHR OUS-SYNCHRONIZA ONIZATION PROTOC OCOL OL IN SHEEP José Franscisco Cox 1 , Evelyn N Lara 2 , Andrea F Leiva 3 , H Muñoz 4 , Rodrigo V Allende 5 & Fernando R Saravia 6 1,2,3,4,5,6 UNIVERSIDAD DE CONCEPCIÓN, CHILLÁN, CHILE. Estradiol benzoate (EB) has been used in cattle to control follicular emergence and to potentiate progesterone to accelerate dominant follicle turnover. The study was aimed to study the effect of EB at CIDR insertion on ovulatory follicular traits and interval to ovulation during the mid breeding season in sheep in ewes subjected to short-term progesterone protocol for estrous synchronization. Fifty four ewes under controlled feeding were synchronized by the insertion of intravaginal progesterone-release device (CIDR G, 0.3 g progesterone, Pfizer) for 6 days combined to cloprostenol (0.125 mg im Estrumate, Intervet) and eCG (400 IU Novormon, Syntex) at CIDR removal (n = 22) or as before plus 0,5 mg EB at CIDR insertion (n = 32). Antral follicular dynamics was followed daily since CIDR retrieval and each 8 h since 12 h after estrus detection by transrectal ultrasonography using a 10-MHz linear array probe connected to a B-mode scanner (Honda HS 2010Vet, Japan). Follicles = 2,5 mm and CLs that were recorded by sketches on ovarian charts. The preovulatory follicular diameter was measured on ovulated follicles that were later confirmed at CL measure 10 days after estrus. To determine ovulation time, ewes were scanned until ovulation, and the moment of ovulation was defined as the middle point between the last identification of preovulatory follicles and their disappearance. Therefore the interval between both CIDR retrieval and estrus detection to ovulation were the periods in hours between these points. Follicular and CL traits and the interval to estrus and ovulation between treatments were compared by the Student’s t-test and a P < 0.05 was considered significant. The results show that both the size of ovulatory follicles and that of CLs at day 10 after estrus were similar (6.0±0.09 vs.. 6.1±0.13 mm; 11.1 vs.. 10.5 mm in diameter for treated and untreated groups respectively; P > 0.10). However, EB increases the intervals from the end of treatment to estrus and ovulation (40.3±1.84 vs.. 34.1±1.97 h; 70.1±1.91 vs.. 64.1±1.96 h for treated and untreated controls respectively; P < 0.05) and reduces the ovulatory response compared to untreated ewes (82.1% vs. 95.7%; 1.26 vs.. 1.82; for ovulation rate and number of follicles ovulated for treated and untreated ewes respectively; P < 0.05). Cumulative information suggest that estradiol benzoate added at CIDR insertion has an inhibitory effect on FSH profiles that delays the emergence of antral follicles and reduces the ovulatory rate and the number of follicles ovulated in treated ewes without other observable changes in follicular development and in CL development after ovulation. [Funded by Consorcio Ovino (FIA FIC-CS-C-2006-1-P-001-22)]. Keywords: estrous synchronization, estradiol, sheep. A078 FTAI, FTET AND AI TAI INCREASES THE REPRODUCTIVE PERFORMANCE OF LACT CTATING TING PRIMIPAR AROUS ACY CYCLIC CLIC NELORE COWS Rodrigo Ribeiro Cunha 1 , Marilu Martins Gioso 2 , Regis José De Carvalho 3 , Carlos Antônio De Carvalho Fernandes 4 , Miller Pereira Palhão 5 , Marina Resende Pimenta Portinari 6 & Thais Camargo Rossi 7 1,2,3,4,5,6,7 UNIFENAS, ALFENAS, MG, BRAZIL. N The objective of this experiment was to evaluate the efficiency of a Fixed Time Artificial Insemination (TAI) protocol in an attempt to improve the reproductive performance of primiparous lactating acyclic Nelore cows. The experiment was conducted for a period of breeding season (BS) of 180 days. Were used 350 Nelore cows with body condition score (BCS) greater than 2.5 (average 2.65 ± 0.01) and days postpartum average of 67.35 ± 1.0 days. The animals were divided into four groups: G1 (n = 61) and G3 (n = 116) - cows with ovarian follicles smaller than 8mm in diameter and absence of CL. G2 (n = 57) and G4 (n = 116) - cows with follicles greater than 8mm and absence of CL. G1 and G2 remained with the bulls in the management of natural mating (NM) throughout the experimental period, from day 0 of BS. The G3 and G4 were treated (Day 0) with an intravaginal device containing 1.0 g of P4 for 8 days and 2mg (2mL) of estradiol benzoate (EB) IM. The withdrawal of P4 (Day 8) were applied IM 400 IU (2mL) of eCG and 75µg (2mL) of cloprostenol and 24 h later (Day 9), administered 1mg (1mL) IM BE. The TAI was performed between 51-54 h after withdrawal of P4 (Day 10) using semen from a single bull. After five days of the TAI, cows in groups G3 and G4 were submitted with bulls in the management of NM. All animals were evaluated by ultrasound for cyclicity rates (presence of CL) and pregnancy diagnosis at 35 and 180 days after onset of BS. For the analysis of the presence of CL and pregnancy rates at 35 and 180 days, between groups, were performed a chi-square. For the variable: period of service was used ANOVA and Duncan. At 35 days of BS, the G3 (42.24% A) and G4 (51.70%) presented higher rates of pregnancy (P < 0.05) than G1 (0% B) and G2 (35% B). A greater number of ovulations was also found (P < 0.05) in G3 and G4 when compared to animals maintained only during NM (6.56% C, 28.07% B 50.86% A, 62, 93%, for G1, G2, G3 and G4, respectively). Pregnancy rates at 180 days were: 22.95% C (G1), 45.61% B (G2), 40.51% B (G3) and 65.51% A (G4). For percentage of animals with CL at 180 days of MS, the G4 (71.55% A) obtained better results than Groups 1, 2 and 3 (42.62% B 56.14% B 48.27% B, respectively). Additionally, groups of TAI + eCG had shorter periods of service (days, P < 0.05): G1 (134.00 ± 11.70 A), G2 (119.65 ± 8.27 A), G3 (79, 91 ± 5.38 B) and G4 (81.40 ± 4.59 B). The work suggests that the TAI was effective to improve the reproductive efficiency of primiparous lactating acyclic Nelore cows in compared to females subjected only NM. In addition, primiparous cows subjected to TAI at the beginning of BS had the reduced postpartum anoestrus period and increased pregnancy rates than cows subjected only to NM. Keywords: ecg, body score condition, zebu breed. s375
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Proceedings of the 25 th Annual Mee
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A262 Bovine Oocyte Vitrification: E
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