2011 (SBTE) 25th Annual Meeting Proceedings - International ...

Acta Scientiae Veterinariae, 2011. 39(Suppl 1): Abstracts - 25th Annual Meeting SBTE-Brazil. August 2011.
A121 OPU-IVP AND ET
EVAL
ALUATION OF A MEDIUM BASED ON COC
OCONUT
ONUT WATER (COC
OCOS OS NUCIFERA)
TO IN VITRO EMBRYO PRODUCTION OF
BOVINE
Marcela Da Silva Cordeiro 1 , Priscilla Do Carmo De Azevedo Ramos 2 , Nathália Nogueira Da Costa 2 , Thiago Velasco Guimarães Silva 2 , Priscila Di Paula Bessa
Santana 2 , Bruno Baraúna Da Silva 2 , Davi César Nascimento Dos Santos 2 , Andre Luiz Alves De Sá 2 , Stefanne Dhullia Braga Conceicão 2 , Carlos Leonardo De
Aragão Araújo 2 , Simone Do Socorro Damasceno Santos 2 , Moysés Dos Santos Miranda 2 & Otávio Mitio Ohashi 2
1
INSTITUTO FEDERAL DE EDUCAÇÃO, CIÊNCIA E TECNOLOGIA DO PARÁ, ABAETETUBA, PA, BRAZIL. 2 UNIVERSIDADE FEDERAL DO PARÁ, BELEM, PA, BRAZIL.
Studies on the chemical composition of coconut water in natura reveal that some of its features are quite different from those
presented by the TCM 199 (Ramos, et al., 2010, Acta Scientiae Veterinariae, 38: 764) which limits its use as a culture medium in IVP. Therefore
the aim of this study was to evaluate a supplemented medium based on coconut water (S-BCWM) to be used in the process of IVM, IVF and
IVC. The S-BCWM consisted of 20 mL of in natura coconut water (5 months of age) + 80 mL of ultrapure water + osmolytes (0.46g NaCl and
0.018g MgSO4, values determined in a previous analysis of chemical composition of coconut water in natura). Amino acids, myo-inositol, trisodium
citrate and sodium bicarbonate were added according to basic formulation of the SOF (Tervit et al., 1972 Journal Reproduction Fertility,
30: 493-497). For IVM period the TCM 199 and S-BCWM media were supplemented with 0.5µg/mL FSH, 50µg/ml LH, 50µg/mL of
gentamicin, 22µg/mL of pyruvate and 10% FCS. In the IVF the SOF medium (without glucose) and S-BCWM were add with 10µg/mL heparin,
2µM penicillamine, 1µM hypotaurine, 0.25 µM epinephrine, 50µg/mL of gentamicin, 22µg/mL of pyruvate and 6 mg/mL BSA. In the
process of the IVC the SOF (1 mM glucose) and S-BCWM were supplemented with 6 mg/mL BSA, 10 µg/mL insulin, 50µg/mL of gentamicin
and 10% FCS. COCs from slaughterhouse were selected and randomly distributed among the following groups: G1 (IVM in TCM199, IVF
in IVC and IVC in SOF), G2 (IVM in S-BCWM, IVF in SOF and IVC in SOF), G3 (IVM in TCM 199, IVF in S-BCWM and IVC in SOF)
and G4 (IVM in TCM199, IVF in SOF and IVC in S-BCWM). Fertilization was performed 18 h after the onset of IVM with semen from a
single bull (2x10 6 sperm/mL). After 30h of IVF, presumptive zygotes were transferred to culture media drops for co-culture with a cumulus
cells monolayer. IVP process was performed in incubator with 5% CO 2
, at 38.5°C with high humidity. Fertilization, cleavage and blastocyst
rates were analyzed by ANOVA (significance level of 5%). It was observed that the maturation of oocytes resulted in S-BCWM Cleavage
(82.50 ± 5.2%) and blastocysts (36.07 ± 8.8%) similar to those obtained by maturation in TCM 199 (82.61 ± 6.7% to 41.47 ± 7.9%, P >
0.05). However, the use of S-BCWM during the IVF period blocked the fertilization process, once 100% of the oocytes were arrested in MII
stage after 18h IVF. When S-BCWM was used for embryo cultivation there was no embryonic development. Therefore, the S-BCWM can
be an option only as a maturation medium in the process of IVP of bovine. Acknowledgements: FAPESPA and UNOPAR.
Keywords: coconut water, cocos nucifera, ivp.
A122 OPU-IVP AND ET
EVAL
ALUATION OF A NEW METHOD FOR IDENTIFYING ENDOMETRITIS IN ANESTROUS MARES THROUGH
ULTR
TRASSONOGR
ASSONOGRAPHY
APHY
Gabriel Maksoud Greco 1 , Leticia Mendes Pupio Maia 1 , Maria Manoela Castro Chaves 2 & Marco Antonio Alvarenga 42
N
1
MÉDICO VETERINÁRIO AUTÔNOMO, CONSELHEIRO LAFAIETE, MG, BRAZIL. 2 UNIVERSIDADE ESTADUAL PAULISTA, BOTUCATU, SP, BRAZIL.
At the beginning and end of the breeding season, when the percentage of recipient mares showing normal estrous cycles is low
compared to donors, progesterone-treated acyclic recipients have been routinely used in embryo transfer programs. However, anestrous
recipients are often selected for treatment through the evaluation of their body score condition and reproductive history, since their poor uterine
tone and endometrial atrophy usually impair it´s evaluation through ultrassonography and rectal palpation. The objective of the present study was
to test the hypothesis that a single estrogen injection could aid in the ultrassographical identification of endometritis in anestrous mares. Twentyfour
anestrous mares that did not show air, endometrial edema or uterine fluid through ultrassography were submitted for endometrial cytology
with a sterile uterine brush. A minimum of ten fields were evaluated per Giemsa-stained slide. Mares were classified as positive for cytology
(moderate endometritis) when more than two neutrophils were microscopically identified per field under oil immersion (×100). Animals were
subsequently injected with 5.0 mg of estradiol benzoate (Estrogin ® , Farmavet, São Paulo, Brazil) I.M., being evaluated through ultrassonography
past 48 h to detect uterine fluid accumulation. The presence and depth of intra-uterine fluid was recorded. Results were statistically analyzed
through the Chi-square test, significance levels set as P < 0.05. Of the twenty-four anestrous mares, eleven were positive and thirteen negative
for cytology. All of them presented some grade of endometrial edema 48 h post-injection. Intra-uterine fluid was detected in all of the positive
cytology mares (100%; 11/11), which was fairly echogenic in three (27.3%; 3/11) and had a depth of a least 20 mm in eight (72.7%; 8/11). On
the other hand, five of the thirteen negative cytology mares (38.5%; 5/13) showed fluid accumulation and only a single mare (20.0%; 1/5) had
more than 20 mm of uterine fluid. Positive cytology mares had higher chances of accumulating intra-uterine fluid, which can exceed 20 mm in
depth (P < 0.05). In conclusion, mares showing uterine fluid 48 h post-treatment with 5.0 mg of estradiol benzoate should not be used as embryo
recipients, for they have higher chances of suffering from endometritis. Moreover, authors suggest that this method should be routinely
implemented during reproductive evaluations of anestrous recipient mares, especially prior to purchase.
Keywords: mare, anestrous, endometritis.
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