2011 (SBTE) 25th Annual Meeting Proceedings - International ...

Acta Scientiae Veterinariae, 2011. 39(Suppl 1): Abstracts - 25th Annual Meeting SBTE-Brazil. August 2011.
A123 OPU-IVP AND ET
HEMODINAMIC EVAL
ALUATION OF THE UTERUS US IN MARES USED AS RECIPIENT: PAR
ARTIAL RESULTS
Fernanda Saules Ignácio, , Jair Camargo Ferreira, Roberta Pahim De Melo, Natalia Claro De Abreu, Gustavo Henrique Marques Araújo & Cezinande Meira
UNESP-BOTUCATU, BOTUCATU, SP, BRAZIL.
An important limitant factor in embryo transfer programs is the number and quality of available recipient mares during the
breeding season. Real time doppler ultrassonography has been recent used to study uterine hemodynamic. However, this is the fisrt report
of Doppler ultrassonography evaluation in recipient mares. The aim of this work was to evaluate recipient mares using power Doppler. A total
of seven recipient mares were ultrassonographicaly followed for ovulation induction (1600IU of hCG). Inovulation was done among D4 to
D6 (D0 = ovulation). Vascular perfusion and spectral Doppler data from vessels of the mesometrium attachment were daily collected from
horns separately (right and left) from D0 to D15. In the pregnant mares (5/7), embryo was located to compare horns with (+) and without (-
) embryo from D9 to D15. Vascular perfusion of both horns were subjectively estimated by scoring (from 1 to 4) the extent of colored areas
in the endometrium during real-time cross-sectioning of the middle segment of each horn during continuous scan of 1 min. For the spectral
mode, one cardiac cycle was arbitrarily chosen from each of the three scans made. Analysis of variance followed by Tukey (P < 0.05) were
used for comparison between right and left horns, pulsatility (PI) and resistance (RI) indexes. Analysis of variance followed by Kruskal-Wallis
were used for comparing (+) and (-) horns. For evaluation of vascular perfusion during the days, Student-Newman-Keuls test was used. For
vascular perfusion, RI and PI Statistical differences were not detected (P > 0.05) when right and left horns were compared, but difference (P
< 0.05) along the days was detected. However, this difference was not detected by Tukey test for the RI. An increase in uterine vascular
perfusion in recipient mares during the days of inovulation (PI at D5 = 0.96±0.10) and, in pregnant mares, a gradual increase kept until D15
(PI = 0,75±0,75). Even though, more studies are still needed to confirm and to add more information to these findings. Uterine hemodynamic
evaluation using the power Doppler and Spectral Doppler showed a great potential for a reliable evaluation of recipient mares and for the
transferred embryos.
Keywords: embryo transfer, recipient, doppler.
A124 OPU-IVP AND ET
COMP
OMPARISON OF THE PREGNANCY RATE OF EMBRYOS COMING OF OVULATION IN MARES SINGLE OR DOUBLE RACE POLO
ARGENTINO
Talita Dias Dos Reis
1 , Mar
arcos Massao Higuti 1 , Jamile Toth De Paula
1 , Suellen Migue
iguez Gonzále
onzález 1 , Eduar
duardo do Ber
ernar
nardi
2 & Thales Ric
icar
ardo Rigo Bar
arreir
eiros
1
1
UNIVERSIDADE ESTADUAL DO NORTE DO PARANÁ, BANDEIRANTES, PR, BRAZIL. 2 CENTRAL DE REPRODUÇÃO EQUINA-MALALHUE, CASBAS, ARGENTINA.
The aim of this study was to evaluate the pregnancy rate of embryos from double ovulation or simple. The study was conducted
through analysis of results in Equine Reproduction Center-Malalhue Casbas in the city of the province of Buenos Aires, Argentina. Experimental
period was the season of 2010 to February 2011. We used 90 mares bred Polo Argentino as embryo donors, and 247 mares breed with embryo
recipients. Donors were inseminated with semen from 14 stallions Polo Argentino. The donors were examined by transrectal ultrasonography
(Chisone D600, 7.0 MHz, China) every 24 h and were inseminated with fresh semen and sperm concentration of 800x106, when the largest
follicle had 35 mm in diameter and uterine edema grade 2 (scale of 0 to 3), repeating the procedure every 48 h until the time of ovulation. The
collection of embryos were seven, eight or nine days after ovulation and the embryos were transferred into recipients that ovulated the same day
or within five days after the donors. The results were analyzed by chi-square test (P < 0,05). 315 samples were performed, resulting in 69,2%
(219/315) recoveries of embryos, which resulted in the recovery of two embryos in 9,5% (30/315) of procedures, and increased 13,7% (30 /
219) of embryos recovered. The pregnancy rate was higher for embryos from double ovulation in relation to embryos from single ovulation
(83,3%, 50/60 vs. 64,7%, 103/159). From the results presented it is concluded that embryos obtained by double ovulation can increase the
pregnancy rate in embryo transfer programs.
Keywords: embryo transfer, mares, ovulation.
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