2011 (SBTE) 25th Annual Meeting Proceedings - International ...

Acta Scientiae Veterinariae, 2011. 39(Suppl 1): Abstracts - 25th Annual Meeting SBTE-Brazil. August 2011.
A067 FOLLICULOGENESIS, OOGENESIS AND SUPEROVULATION
FIRST SUCCESSFUL CESSFUL ESTROUS SYNCHRONIZA
ONIZATION USING A PROST
OSTAGL
GLANDIN ANALOGUE IN COLL
OLLARED PECCARIES
(TAYASSU
ASSU TAJA
AJACU)
Alexandre Rodrigues Silva 1 , Keila Moreira Maia 2 , Mariana Araújo Silva 3 , Gislayne Christianne Xavier Peixoto 4 m José Artur Brilhante Bezerra 5 m Andréia
Maria Da Silva 6 & Moacir Franco Oliveira 7
1,2,3,4,5,6
LCGA - UFERSA, MOSSORO, RN, BRAZIL. 7 CEMAS - UFERSA, MOSSORO, RN, BRAZIL.
The collared peccaries (Tayassu tajacu) are small mammals present in the whole Brazilian territory. Recently, they were classified
as vulnerable to extinction, mainly due to the predatory hunting and deforestation. Its sustainable breeding under captivity could be an
alternative for its conservation, as well as for production of meat and pelts that are good accepted in the international market. The development
of methodologies for the control of estrous cycle could assist in the implementation of biotechniques such as artificial insemination and
embryo transfer. Therefore, it was aimed to develop a program for the estrous synchronization in captive female collared peccaries. The
estrous cycle of five females, aging 18 ± 0.4 months and weighting 20.6 ± 1.1 kg, bred under captivity in Centre of Multiplication of Wild
Animals of UFERSA, were monitored through estrogen dosage using ELISA and evaluation of external genitalia during 15 days. Then, a
prostaglandin F-2á analogue, cloprostenol (Veteglan ® , Hertape Calier, Belo Horizonte, Brazil) was administered at a 60 µg/Kg dosage, IM, and
repeated after nine days. After first application, the monitoring of the cycle continues for 20 days, every two days, for the verification of
estrous. The data were analyzed by descriptive analysis (Statview 5.0, Cary, USA). By monitoring the natural cycle, it was observed that
three females were in follicular phase when the protocol begun. At 8 ± 1.7 days following the first prostaglandin administration, an estrogen
peak was verified in three females that presented 27.7 ± 11.1 pg/mL, but only two of that presented external sings of estrous (66.7%). It was
noted that such females who responded to the first application, only one was demonstrated in follicular phase during the administration of
drugs. As a consequence of the second application, all animals achieved an estrogenic peak at 9.2 ± 1.1 days, showing levels of 28.1 ± 10.4 pg/
mL of estrogen. Four of the five females (80%) showed external signs of estrus, such as vulvar opening, hyperemic vaginal mucosa and the
presence of vaginal mucus. The response to the treatment was considered late when compared to domestic species, and it is suggested that
higher prostaglandin doses could reduce the interval for estrous manifestation. It should be emphasized that this is the first description of a
program for the estrous cycle control in collared peccaries, and the efficiency of the prostaglandin analogue use for the estrous synchronization
of this species was demonstrated.
Keywords: estrous cycle, peccary, synchronization
A068 FOLLICULOGENESIS, OOGENESIS AND SUPEROVULATION
CELLUL
ULAR PROLIFER
OLIFERATION OF POLIOVULAR FOLLICLES IN ADULT NELORE OVARIES
Katia Cristina Silva-Santos 1 , Gustavo Martins Gomes Dos Santos 2 , Reginaldo Luís Oliveira 3 , Letícia Schmidt Siloto 4 , Ana Paula Frederico Rogrigues Loureiro
Bracarense 5 & Marcelo Marcondes Seneda 6
1,2,3,4,5,6
UEL, LONDRINA, PR, BRAZIL.
Polyovular follicles identification has already been reported in many species. Thereafter, little information is available in the
literature about these follicles in the ovaries from Nelore adult females (Silva-Santos et al., 2011, Theriogenology, in press) and their function
remains unclear. This way, aiming to evaluate cellular proliferation of polyovular follicles in the ovaries from Bos taurus indicus animals,
ovaries were collected at abattoirs from adult Nelore females (24-30-months-old, n = 21), cut longitudinally, fixed in Bouin’s solution and
processed for histology and imunohistochemical. The ovaries halves were dehydrated in alcohol, cleared in xilol, embbebed in paraffin and
serially sectioned at 5µm. ovarian tissue was processed for histology and imunohistochemical. After being identified by histology, sections
were stained with antibodies against proliferating cell nuclear antigen (PCNA, DAKO, Carpinteria, CA, USA), as described previously
(Oktay et al., 1995, Biol. Reprod. 53:295-30). Polyovular follicles were classified according to the shape and number of granulosa cells. From
ovaries analyzed by histology, four (19%) presented polyovular follicles containing two oocytes nuclei at primordial and primary stage. All
polyovular follicles (n = 10) were PCNA positive, which indicates activity and proliferation of these structures. The majority of granulosa
cells and oocyte nuclei from these follicles were PCNA positive, except two polyovular follicles in the same ovary, which one oocyte nuclei
was PCNA positive and the other one was not, although both had PCNA-positive granulosa cells. It was concluded that polyovular follicles
from adult Nelore females are proliferating and that these structures might be related with folliculogenesis.
Keywords: polyovular follicles, proliferation, Bos taurus indicus.
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