2011 (SBTE) 25th Annual Meeting Proceedings - International ...

Acta Scientiae Veterinariae, 2011. 39(Suppl 1): Abstracts - 25th Annual Meeting SBTE-Brazil. August 2011.
A157 OPU-IVP AND ET
PREGNANCY RATE IN MANGALAR
ARGA MARCHADOR RECIPIENTS AT DIFFERENT AGES
WITH OR WITHOUT PROGESTER
OGESTERONE
ONE
SUPPLEMENTATION
TION
Sávio Oliveira Paiva, Carlos Gramatico Massoni de Oliveira, Paula Cardoso Almeida, Marcus Andre Ferreira Sá, Renato Guerra Castro Junqueira & Júlio
César Ferraz Jacob
UFRRJ, SEROPEDICA, RJ, BRAZIL.
The Embryo Transfer (ET) in horses has been used due to the advantage of generating more than a product from high value
livestock mares per year. However, several factors affect the success of the TE and the physiological moment of the recipientis a major
determinant of success that depends on the degree of synchrony between donor and recipient during estrus, due to continuous secretion of
progesterone, which is essential for the initiation and maintenance of pregnancy. This study aimed to evaluate the pregnancy rate of recipients
in different age groups with and without the use of long acting progesterone (P4 LA). The project was conducted at a Central Commercial
Embryo Transfer. A total of 52 Mangalarga Marchador recipients were used and alocated to four groups according to age and administration
of progesterone: G1: 2 to 5 years without P4 (n = 18); G2: 2 to 5 years with P4 (n = 9); G3: 6 to 15 years without P4 (n = 44); and G4: 6 to
15 years with P4 (n = 17). Embryos were recovered between 7 and 9 days after ovulation of the donor (D0); embryos classified as excellent
or very good were transferred. Recipients were evaluated by rectal palpation and transrectal ultrasonography to evaluate the uterine condition,
recipients that did not have good uterine tone and adequate uterine echotexture were treated with 1500 mg P4 LA IM on the day of ET.
Pregnancy diagnosis was performed at 15 days after ovulation in the donors and repeated at 30 and 45 days of gestation to the early embryonic
loss. The results were 55.6% (10/18), 22.2% (2/9), 38.6% (17/44), 58.8 (10/17) for G1, G2 , G3 and G4, respectively. The design was
completely randomized using analysis of variance (ANOVA) and Tukey test, at 5% significance. There was no statistical difference between
groups (P > 0.05). Therefore, we conclude that the use of progesterone in recipients, regardless of age, did not increase the pregnancy rate.
Keywords: pregnancy rates, progesterone, different age.
A158 OPU-IVP AND ET
CONCEPTION RATES OF IN VITRO PRODUCED BOVINE EMBRYOS
WITH CONVENTIONAL AND THAWED-SEXED SPERM OF
NELLORE BULLS
Julio Cesar Barbosa da Silva 1 , Wagner Kodato Okabe 1 , Maria Clara Costa Mattos 2 , Bruno Valente Sanches 2 , José Henrique Fortes Pontes 2 , André Gomiero
Rigo 2 , Lucas Lopes Moino 2 , Rodrigo Mendes Untura 2 & Andrea Cristina Basso 2
1
EMBRYO SYS REPRODUÇÃO ANIMAL, OURO FINO, MG, BRAZIL. 2 IN VITRO BRAZIL, MOGI-MIRIM, SP, BRAZIL.
Thawed-sexed sperm is a biotechnology that has been widely used on IVP bovine embryo routine. This process allowed sexing
of sperm from frozen conventional doses. Especially, for bulls that have not produced semen or have died. However, conception rates of IVP
embryos with thawed-sexed sperm are still variable. The aim of this report was to present the conception rates of IVP embryos using
conventional and thawed-sexed sperm from Nellore bulls, on the same commercial farm, during January 2010 to April 2011. The experiment
was conducted at Eldorado´s farm, Itapetininga, SP, Brazil. Seven days after in vitro fertilization, embryos produced with conventional (n = 595)
and thawed-sexed sperm (n = 184) from four Nellore bulls were transferred to synchronized recipients.The pregnancy diagnosis and fetal sexing
were performed by trans-rectal ultrasound 30 and 60 days after transfer, respectively. The conception rates of embryos produced with
conventional sperm were 50.8% and 47.5% at 30 and 60 days, respectively. The conception rates of embryos produced with thawed-sexed
sperm were 51.1% and 44.3% at 30 and 60 days, respectively. Embryo loss was similar between IVP embryos with conventional (7.0%) and
thawed-sexed (7.4%) sperm. Finally, embryos produced using conventional sperm resulted in 42% of females and 58% males. However,
embryos produced using thawed-sexed sperm resulted in 94% of females and 6% of males. These results demonstrate that the use of thawedsexed
sperm on IVP bovine embryos can be applicable in large commercial scale, because it presents a high sexing accuracy and it seems not
compromise the conception rates and embryo loss. [Acknowledgements: Golin Group, Itapetininga, SP, Brazil].
Keywords: embryo, sexed sperm, ivf.
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