2011 (SBTE) 25th Annual Meeting Proceedings - International ...

Acta Scientiae Veterinariae, 2011. 39(Suppl 1): Abstracts - 25th Annual Meeting SBTE-Brazil. August 2011.
A129 OPU-IVP AND ET
EFFECT OF HIGH ENERGY DIET ON METABOLIC,
ENDOCRINE AND REPRODUCTIVE PAR
ARAMETERS AMETERS ON BOS INDICUS AND BOS
TAUR
URUS
US COWS
José Nelio S. Sales
1 ; Lilian Tam
amy Iguma 2 ; Car
arolina Cap
apobiango R. Quin
uintão
3 ; Mar
arco A.S. Gama
4 ; Célio Freitas
5 ; Michele Munk Per
ereir
eira 6 ; Luiz Sérgio Almeida
Camargo 7 ; João Henrique Moreira Viana 8 ; Pietro Sampaio Baruselli 9
1,9
UNIVERSIDADE DE SÃO PAULO, SÃO PAULO, SP, BRAZIL. 2,3,4,5,6,7,8 EMBRAPA, JUIZ DE FORA, MG, BRAZIL. 5,10 EMBRAPA, VALENÇA, RJ, BRAZIL.
The effect of different energy levels in diet [maintenance (M) and high energy (1.7M)] on metabolic, endocrine and reproductive
parameters of non lactating Bos indicus (n = 14; Gir) and Bos taurus (n = 14; Holstein - HPB) cows submitted to ultrasound guided ovum pick
up (OPU) followed by in vitro embryo production (PIV) was evaluated. The oocyte donors were housed in Tie Stall System and the diets were
given twice daily (8:00 AM e 4:00 PM). During 21 days prior to the beginning of experiment, animals were fed with the maintenance diet for
their adaptation. After this period, the experimental diets (M and 1.7M) were given and cows (Gir and HPB) were submitted to nine OPU
procedures, 14 days apart each. The donors were synchronized before OPU (D0 - 2mg EB + norgestomet ear implant; D5 - OPU). There were
no interaction between breeds and energy level in diet and effect of diet for the qualitative or quantitative variables. However, Bos indicus cows
showed higher number of recovered structures and better oocyte quality when compared to Bos taurus donors. Similar to what was found in
oocyte quality and number, the in vitro embryo production also did not differ between diets and it was observed that Bos indicus cows had higher
production of embryos. However, we verified that the high energy reduced in vitro embryo production in Bos indicus cows after 60 days of high
energy diet. Moreover, Gir cows that recevied high energy showed higher transcript abundance for HSP70.1, GLUT1, IGF1R e IGF2R genes.
Cows fed with excess of energy in diet presented higher serum and follicular fluid concentrations of glucose and cholesterol. However, the ureic
nitrogen concentration was higher in cows which received maintenance diet. When comparing the two genetic groups, it was observed that Bos
indicus cows presented higher concentrations of glucose, cholesterol, NEFA and ureic nitrogen both in follicular fluid and in blood. Furthermore,
cows that presented decreased blastocyst rate (high energy - Bos indicus) exhibited high follicular fluid concentrations of insulin. In conclusion,
increasing energy in diet did not interfere in oocyte number and quality. However, the high energy reduced the in vitro embryo production in Bos
indicus cows after 60 days of diet. Moreover, Bos indicus cows showed better oocyte quality, higher number of viable oocytes and increased in
vitro embryo production than Bos taurus.
Keywords: opu-fiv, energy, oocytes.
A130 OPU-IVP AND ET
EFFECT OF THE SPERM SELECTION WITH ISOLATE
® OR PERCOLL
® ON SPERM QUALIT
ALITY AND IN VITRO BOVINE
EMBRYO DEVEL
VELOPMENT FOR FROZEN-THA
OZEN-THAWED SEXED AND NON-SEXED SEMEN
Paula Ro drigue
iguez V illamil 1 , M aria Eugenia Gar
arcia Gome
omez 2 , Mar
ariana Fer
ernande
nandez Tar
aranc
anco 2 , M ariana Caccia
2 & Gabr
abriel Bó 1
1
INSTITUTO DE REPRODUCCION ANIMAL DE CORDOBA, CORDOBA, ARGENTINA. 2 UNIVERSIDAD DE VILLA MARIA, VILLA DEL ROSARIO, ARGENTINA.
N
The aim of this study was to compare the effects of spermatozoa separation techniques Isolate ® (Irving-Scientific, California, USA)
and Percoll ® (Nutricell, SP, Brazil), on sperm quality and in vitro embryo production using sexed and non-sexed semen. Oocytes (n = 5046) were
obtained from slaughterhouse ovaries and fertilized with frozen/thawed sexed or unsexed semen of four Holstein bulls. The sperm quality
(motility, concentration, morphology and membrane integrity) were evaluated and compared before and after sperm selection by the two
methods. Oocytes were maturated in TCM-199 supplemented with 0.4% of BSA for 24 h in controlled atmosphere and those matured were
selected and randomly allocated into four different groups. Group 1: oocytes fertilized with sexed semen selected by Percoll ® ; Group 2: oocytes
fertilized with sexed semen selected by Isolate ® ; Group 3: oocytes fertilized with non-sexed semen selected by Percoll ® ; Group 4: oocytes
fertilized with non-sexed semen selected by Isolate ® . Fertilization was performed in Fert-talp medium during 18 h at same conditions as
maturation. Consequently, presumptive zygotes were cultured for 7 days in SOF medium in a 39°C humidified incubator with 5% CO2, 5% O2
and 90% N2. The results of cleavage and embryo production were evaluated on Day 2 and 7 after fertilization. All percentages were transformed
by square root and compared by ANOVA. Regardless of sperm selection technique, sperm motility and percentage of normal sperm increased
(P < 0.005) compared with the initial sperm parameters. For non-sexed semen, Percoll ® gradient increased the recovery rate of sperm (57.3 ±
2.7) compared with Isolate ® (46.0 ± 1.8, P < 0.05). Furthermore, sperm selected by Isolate ® presented significant improvements compared with
Percoll ® gradient, on membrane integrity of sexed (41.0 ± 0.6 vs. 38.8 ± 0.8) and non-sexed semen (60.8 ± 1.6 vs. 58.8 ± 0.5, P < 0.05). Finally,
blastocyst development rates were greater (P < 0.05) for sexed (Group 2: 14.0 ± 1.0) or non-sexed semen (Group 4: 22.0 ± 1.1) selected by
Isolate ® when compared to those produced with sexed (Group 1: 10.5 ± 1.5) or non-sexed semen (Group 3: 17.0 ± 2.1) selected with Percoll ® .
In conclusion, Isolate ® may be a better alternative than Percoll ® as a sperm selection method for IVP systems using sexed or non-sexed bovine
semen.
Keywords: selection, sexed, non-sexed.
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