2011 (SBTE) 25th Annual Meeting Proceedings - International ...

Acta Scientiae Veterinariae, 2011. 39(Suppl 1): Abstracts - 25th Annual Meeting SBTE-Brazil. August 2011.
A033 MALE REPRODUCTIVE PHYSIOLOGY AND SEMEN TECHNOLOGY
THE INFLUENCE OF CULTURE MEDIUM ON OVIDUCT CELLS SPERM VIABILITY IN CATTLE
TLE
Patrícia da Silva Valleriote, Helga Fernandes Gomes, Carla Sobrinho Paes de Carvalho, Fausto Paes de Carvalho & Angelo José Burla Dias
UENF, CAMPOS DOS GOYTACAZES, RJ, BRAZIL.
The aim of this study was to evaluate the influence of the secretions from oviduct cells on physical and functional plasma
membrane of bovine spermatozoa. The oviducts were obtained from slaughterhouses and dissected in the laboratory. The oviduct epithelial
cells were grown in culture bottles containing 5,0 mL of culture medium and maintained at 38,5°C; 5% CO 2
for five days. The medium was
centrifuged and the supernatant was concentrated 10 times in a protein concentrator (Amicon, Millipore). The total protein concentration was
determined by the method of Bradford (1976). The straws of semen were thawed and submited to a Percoll gradient 45/90%. The sperm were
resuspended in TALP-sp supplemented with BSA, at final concentration of 50x10 6 /ml and divided into three groups: T Ct (TALP-sp); T Hep
(TALP-sp + 10 µg/mL heparin); T Ov (TALP-sp + 3,6 µg/mL of culture medium of oviduct cells). The semen was evaluated at 0h and after 3h
incubation in relation to total motility (TM), progressive motility (PM), vigor (V) and hyposmotic test (TH). The samples were incubated
in an incubator with an atmosphere of 5% CO 2
; 38,5°C, for 3 h. In 0h the treatments T Ct , T Hep and T Ov presented similar results for the MT
(85,0±11,2%; 80,0±10,4% and 86,4±4,75%, respectively). The results of the MP were 65,7 ± 23,1% (T Ct ); 58,6±19,3% (T Hep ) and
69,3±13,7% (T Ov ), respectively. In the three treatments V was equal to 4,0. After 3h incubation, the MT values (mean ± SD) of T Ct , T Hep and
T Ov were reduced in relation to 0h (65,4±13,0% ab ; 52,9±18,9% a e 75,0±5,0% b respectively). After three hours of incubation, we observed the
following values of MP: T Ct (40,9 ± 21,6%); T Hep (36,4 ± 23,9%) and T Ov (48,6 ± 22,5%). The V values were: T Ct (3,1 ± 0,9%); T Hep (3,3 ±
0,8%) and T Ov (3,4 ± 0,8%). The percentage of viable cells in TH 0h was 71,9±15,9% (T Ct ), 70,9±7,9% (T Hep ) and 66,7±20,8% (T Ov ). After
3h incubation, we observed the following results: 64,3±8,9% (T Ct ), 65,1± 14,7% (T Hep ) and 64,4±16,3% (T Ov ). The secretions of bovine
oviduct cells promoted increased values of MT sperm after 3h of incubation, indicating that these secretions may prolong the sperm viability.
[Acknowledgment: FAPERJ].
Keywords: spermatozoa, cell culture, seminal parameters.
A034 MALE REPRODUCTIVE PHYSIOLOGY AND SEMEN TECHNOLOGY
INFLUENCE OF PERIOD OF THE YEAR IN SEMINAL QUALIT
ALITY OF CAPTIVE COLL
OLLARED PECARIES (PECARI
TAJA
AJACU) RAISED IN
THE STATE TE OF PAR
ARA
Priscila Reis Kahwage 1 , Alexandre Rossetto Garcia 2 , Diva Anelie De Araujo Guimarães 3 , Otávio Mitio Ohashi 4 , Natalia Inagaki Albuquerque 5 , Mario Mansour
Pinheiro Bartha 6 & Jakeline Dos Santos Pessoa 7
1,3,4,6
UNIVERSIDADE FEDERAL DO PARÁ, BELEM, PA, BRAZIL. 2,5 EMBRAPA AMAZÔNIA ORIENTAL, BELÉM, PA, BRAZIL. 7 UNIVERSIDADE FEDERAL RURAL DA AMAZÔNIA, BELÉM, PA,
BRAZIL.
The continuous assessment of collared peccaries (Pecari tajacu) semen characteristics might be useful to the development of semen
collection procedures and gamete conservation during most favorable periods of the year. On the other hand, the environment in the Amazon
region influences the reproductive characteristics and the behavior of the majority of domestic and wildlife species. Therefore, the aim of this
research was evaluating physical and morphological characteristics of collared peccaries semen, considering two different periods of the year and
its interference on semen quality. Eight adult males (52 ± 14.9 months and 19.8 ± 2.1 kg) from Embrapa Eastern Amazon (Belém, PA) herd were
used as semen donors. Semen collection were carried out each fifteen days by electroejaculation, according Garcia et al. (2009, Revista Brasileira
de Reprodução Animal, Supl.1, 462). Seminal samples (n=65) were evaluated for volume (mL), sperm concentration (million sptz/mL), pH (0-
14), progressive motility (%), vigour (0-5), viability (%), major, minor and total sperm defects (%). The experiment occurred uring 17 months
under tropical humid climatic type. Months from December to May were considered as “Intense Rainy Period” (IRP) while months from June
to November were considered as “Soft Rainy Period” (SRP), according to Pachêco and Bastos (2007, Série Documentos - Embrapa Eastern
Amazon, 300, 18). The effect of period over the seminal characteristics was evaluated using Student t test (P < 0.05). There was significant
difference in the ejaculated volume (0.75 ± 0.5a mL in IRP X 0.3 ± 0.6b mL in SRP, P < 0.05). However, this feature depends on the semen
collection method adopted and is also related to individual responses to electrical stimuli. Seminal features observed during IRP and SRP were,
respectively: concentration of 109.8 ± 95.7 million sptz/mL and 167.2 ± 185 million sptz/mL, pH of 7.8 ± 0.6 and 7.8 ± 0.7, motility of 55.1 ±
28, 9% and 53.5 ± 28.5%; vigour of 2.3 ± 0.9 and 2.0 ± 0.7, and viability of 56.1 ± 28.8% and 56.6 ± 28.8% (P > 0.05). Morphologically, there
was 23.7 ± 14.0% and 21.7 ± 12.7% of major defects, 9.6 ± 7.7% and 8.6 ± 5.7% of minor defect and 33.3 ± 13.5% and 30.3 ± 13.7% of total
sperm defects (P > 0.05). Differences between collared peccaries were observed (P < 0.05) for volume (0.3 ± 0.2 mL to 1.9 ± 0.8 mL in IRP,
0.2 ± 0.1 mL to 1.0 ± 0 , 7 mL, in SRP), motility (18.3 ± 27.5% to 85.0 ± 4.0% in IRP and 18.2 ± 19.0% to 80.0 ± 6.1% IN SRP) , viability
(19.6 ± 29.8% to 84.6 ± 3.2% in IRP and 21.1 ± 22.4% to 81.4 ± 7.5% in the SRP) and concentration (55.0 ± 48.0 to 167.19 ± 111.9 milion
sptz/mL, in IRP and 40.53 ± 19.6 to 228.0 ± 150.0 million sptz/ Ml, in SRP). However, studied periods did not influenced on individual variation
(P > 0.05).The absence of difference in the seminal features between periods reinforces the adaptability of collared peccaries to environmental
variations of tropical humid climate. Thus, it was concluded that the period of the year in evaluated region did not interfere on physical and
morphological seminal characteristics of Pecari tajacu. This fact enables the continuous research about biotechnologies of reproduction in this
specie and leads to future establishment of ex situ germplasm collections.
Keywords: andrology, spermatozoa, wildlife.
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