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Acta Scientiae Veterinariae, 2011. 39(Suppl 1): Abstracts - 25th Annual Meeting SBTE-Brazil. August 2011. A163 EMBRYOLOGY, BIOLOGY OF DEVELOPMENT AND PHYSIOLOGY OF REPRODUCTION PHYSIOLOGICAL AND ENDOCRINE CHANGES OF BOVINE CORPUS LUTEUM AFTER CHALLENGES WITH SUB-DOSE OF CLOPROTENOL SODIUM Eduardo Trevisol 1 , Wolff Camargo Marques-Filho 2 , Flavia Caroline Destro 3 , Jair Camargo Ferreira 4 , Aline Sousa Camargos 5 , Jackson Barros Amaral 6 , Cezinande Meir eira 7 , Fer ernada C.S.Z. Matt ttos 8 , Rob ober erto Sar artor ori 9 & João C.P. Fer erreir eira 10 1,2 UNESP-BOTUCATU, AMERICANA, SP, BRAZIL. 2,6,7 UNESP-BOTUCATU, CAMPINAS, SP, BRAZIL. 3,4,5,7,8,10 UNESP-BOTUCATU, BOTUCATU, SP, BRAZIL. 4 UNESP-BOTUCATU, SÃO PAULO, SP, BRAZIL. 6 INSTITUTO DE ZOOTECNIA, NOVA ODESSA, SP, BRAZIL. 8 UNESP-BOTUCATU, PIRACICABA, SP, BRAZIL. 9 USP-ESALQ, PIRACICABA, SP, BRAZIL. The action of sub-doses of PGF2α does not always cause CL regression due to some factors, such as cycle phase, concentration and administration form of the product. In some situations in which luteolysis does not occur, it is observed within the early 12-24 h after PGF2α administration, the decrease in plasma progesterone (P4) concentration to values near to 1.0 ng/mL, followed by the subsequent increase of P4 to values similar to those observed before treatment. Due to the importance of this phenomenon to understand the factors that regulate luteolysis, the objective of this study was to evaluate changes in P4 and luteal blood flow after administration of 83.33 µg of cloprostenol sodium on the sixth day of the estrous cycle. Nineteen Caracu mature cows were submitted the following synchronization protocol: Day -9 (D-9 - protocol beginning): 50 µg lecirelin (Gestran Plus ® - Tecnopec - São Paulo – Brazil; IM) and insertion of a P4 intravaginal device (DIB ® - Syntex - Argentina); D-2.5: 500 µg cloprostenol sodium (Sincrocio ® - Ouro Fino, São Paulo, Brazil, IM); D-2; 250 µg cloprostenol sodium (Sincrocio ® , IM) and withdrawn of the intravaginal device; D0: 50 µg lecirelin (Gestran plus ® , IM). On D6, the cows were divided randomly in three experimental groups: G1 (2 mL of saline; IM, n = 6), G2 (two doses of 500 µg cloprostenol sodium 2 h apart; IM, n = 6) and G3 (83,33 µg cloprostenol sodium; IM, n = 7). Data collection were performed with mode B ultrasonography and Power-Doppler evaluations followed by blood collection for P4 measurement at 0, 8, 16, 24, 32, 40 and 48 h after treatment. Data was submitted to ANOVA and for the differences between means the TUKEY test with 5% probability was applied. Treatments influenced P4 concentration, blood flow and luteal volume. On G1, no changes were observed in any varible (P > 0.05). However, on G2, P4 decreased 16 h after treatment (P < 0.001) and maintained until the end of the evaluation period. Blood flow and luteal volume decreased 48 h after treatment in G2 (P = 0.049). On G3, there was a decrease in circulating P4 concentration from the time of treatment until 18 h later (P = 0.025), but after that time, circulating P4 increased and returned to initial values. Despite this observation, blood flow and luteal volume did not change (P > 0.05). Based on these results, we concluded that 1/6 of cloprostenol sodium dose, cause a partial luteolysis. Keywords: bovine, luteolysis, corpus-luteum. A164 EMBRYOLOGY, BIOLOGY OF DEVELOPMENT AND PHYSIOLOGY OF REPRODUCTION CHANGES IN BLOOD CHEMISTRY PROFILES AND ASSOCIATION WITH BIRTHWEIGHT THWEIGHTS IN IVF-DERIVED NEWBORN CAL ALVES Luiz Fer ernando Schütz 1 , Fabiano Car armina minatti Zago 2 , Luis Henr enrique Aguiar 1 , P.C. dos San antos-N os-Net eto 1 , Jamir Machado Júnior 1 , Monic onica Urio 1 , Leonar eonardo Tondello Martins 3 , Diego Fernandes 1 , Willian Braun 1 , Saul Gaudêncio Neto 3 , Cristiano Feltrin 3 , Luciana Relly Bertolini 3 , Ubirajara Maciel da Costa 1 , Fabiana Forell 1 & Marcelo Bertolini 3 1 UDESC / CAV, LAGES, SC, BRAZIL. 2 EPAGRI, LAGES, SC, BRAZIL. 3 UNIFOR, FORTALEZA, CE, BRAZIL. The aim of this study was to compare clinical and blood chemistry parameters between newborn calves produced either in vivo by superovulation (SOV) or in vitro by in vitro fertilization (IVF) during the immediate neonatal period, correlating possible changes in physiological profiles with risk of death in IVF animals. Parturition was pre-induced in nine bovine females on Day 268.6 ± 0.6 of pregnancy by the administration of 8 mg triamcinolone acetonide IM, followed by induction of parturition on Day 275.4 ± 0.6 days with 25 mg dexamethasone acetate and 0.5 mg PGF2á analogue (IM). Four IVF- and five SOV-derived Flemish calves, delivered on Day 276.3 ± 0.6 of gestation, were subjected to sequential blood sampling by jugular venipuncture during the first 6 h ex utero (5, 15, 30, 60, 120, 240, and 360 min) for the analyses of blood gases and electrolyte concentrations, concurrent with the recording of vital clinical signs (rectal temperature, BT; respiratory rate, RR; and hearth rate, HR). Data for groups (IVF and/or SOV vs.. time) were analyzed by ANOVA (Minitab), Proc MIXED (SAS), and simple correlation (P
Acta Scientiae Veterinariae, 2011. 39(Suppl 1): Abstracts - 25th Annual Meeting SBTE-Brazil. August 2011. A165 EMBRYOLOGY, BIOLOGY OF DEVELOPMENT AND PHYSIOLOGY OF REPRODUCTION BREEDING SEASON ANTICIPATION TION IN MARES SUBMITTED TED TO A PROGESTER OGESTERONE ONE INTRAVAGINAL IMPLANT OR ARTIFICIAL LIGHTING Rob ober erta Pahim de Melo elo, Fer ernanda Saules Ignácio, Jair Camar amargo Fer erreir eira, José Nicolau P. Puoli Filho & Cezinande Meir eira FMVZ - UNESP, BOTUCATU, SP, BRAZIL. The present study was done aiming to evaluate the use of progesterone intravaginal implants on mares’ ovulation anticipation and to compare with the use of artificial lighting. For this use, 27 cross-breeding mares were randomly assigned into three groups: P4 (n = 7; progesterone intravaginal implant; Primer ® , 1g of progesterone with no estradiol), lighting (n = 10; artificial lighting program) and control (n = 10; not treated). In P4 group, the progesterone intravaginal implant was introduced and kept for 12 days when transitional phase characteristics were detected. Animals of the lighting group were daily treated with five hours of artificial lighting (5 to 10 pm) for 60 days, beginning at June 21st. For the comparison of the evaluated intervals among P4, lighting and control groups, analysis of variance followed by Tukey test were used. For the first ovulation frequency, a chi-square test was used. The progesterone intravaginal implant reduced the interval until detection of a follicle =35mm when comparing to control group and was similar to mares under artificial lighting program. The findings of =35mm follicles in P4 group indicate that implant had a higher efficiency on follicular growth stimulation. The interval between transitional phase detection to =35mm follicles were similar among groups, but the moment transitional phase were detected was different. All mares in P4 group showed follicles =35mm during treatment to five days post treatment. Even though the largest follicle achieved =35mm, the mean days from removal to ovulation was of 20.6 days, which was higher than 6.6 days found by Newcomb, 2002 (Journal of equine veterinary science. 22, 378-382). This difference is explained by the two mares that showed largest follicles regression. Considering the mean interval (days) between winter solstice and ovulation, lighting treatment was efficient compared to control. The use of progesterone implants showed regular efficiency and it was similar to both other groups. This fact probably happened because of two mares that ovulated in late December and the cause was not established. In relation to frequency of ovulation distribution along months, as lighting treatment as progesterone implant promote ovulations earlier in the breeding season when compared to control (P < 0,05). Concluding, lighting treatment and progesterone implants when used in transitional mares efficient to promote a higher frequency of ovulations early in the breeding season. Keywords: p4, lighting, ovulation. A166 EMBRYOLOGY, BIOLOGY OF DEVELOPMENT AND PHYSIOLOGY OF REPRODUCTION EVAL ALUATION OF HYPOTHAL THALAMIC-PITUIT AMIC-PITUITAR ARY RESPONSIVENESS DURING THE POSTPAR ARTUM NELLORE COWS Cir iro Mor oraes Bar arros os, José Rena enato Cur ury, Rafael August ugusto Satr trapa, Mar arcelo Pegor egorer er, Luzia Apar parecida Trinc inca & Vinicius Pinheir inheiro UNESP, BOTUCATU, SP, BRAZIL. The presence of calf, body condition score, energy balance, number of births (multiparous vs. primiparous) and breed are factors that influence the duration of postpartum anoestrus in beef cows. The objective of the present study was to evaluate, during early postpartum, the time of re-establishment of pituitary LH stocks, mensured by the hypothalamic-pituitary axis responsiveness to exogenous administration of GnRH or estradiol benzoate (EB). Multiparous lactating Nellore cows (Bos indicus, n = 90) were randomly allocated into eight groups, according to the hormonal treatment: EB group (1.0 mg of EB, i.m., n = 7), GnRH group (50 µg lecireline, i.m., n = 17). The EB-SUP (n = 9) and GnRH-SUP (n=11) groups received the same treatments above specified and were supplemented (SUP) with a balanced diet, based on cotton meal and ground corn (3.5 kg/cow/day). Additionally, animals from EB-CR (n = 4) and GnRH-CR (n = 11) received the same treatments of EB and GnRH group respectively and had their calf removed (CR) shortly after parturition. Moreover, two other groups were LH (LH 12.5 mg, i.m., n = 14) and Control group (received saline instead of an ovulation-inducing agent, n = 11). The hormones were administered weekly, from seven days postpartum (±5 days) until the occurrence of the first ovulation, which was determined by the presence of CL during ovarian ultrasonography weekly performed. Blood samples were collected just before and 2 h (GnRH, LH and control groups) or 18 h (EB groups) after hormone or saline (control) administration, in order to determine LH concentration by RIA. Data were analyzed by ANOVA. Means values in days (±SEM) for the first post-partum LH surge were: EB (73.0±5.2); EB-CR (16.7±5.8); EB-SUPL (41.7±6.7); GnRH (32.3±3.0); GnRH- CR (11.0±3.5); GnRH-SUPL (15.6±2.8). There were significant differences (P, 0.05) between groups EB vs.. EB-CR; EB vs. EB-SUP; BE vs.. GnRH; GnRH vs.. GnRH-CR; GnRH vs.. GnRH-SUP, and a tendency between EB-SUPL vs. EB-CR (P < 0.10). Results indicate that from the second week postpartum there is sufficient LH in the pituitary to induce ovulation after GnRH or EB administration. However, the cows from EB group ovulated later than animals from the other groups, possibly due to the sensitivity of the hypothalamus to negative feedback of estrogens, inhibiting the pre-ovulatory LH surge. Additionally, calf removal and food supplementation reduced in 2 to 4 weeks the time of the first postpartum LH surge induced by GnRH or EB in Nellore cows. Keywords: post-partum, lh, bovine. N s419
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