2011 (SBTE) 25th Annual Meeting Proceedings - International ...

Acta Scientiae Veterinariae, 2011. 39(Suppl 1): Abstracts - 25th Annual Meeting SBTE-Brazil. August 2011.
A249 SUPPORTIVE BIOTECHNOLOGIES: CRYOPRESERVATION AND CRYOBIOLOGY, IMAGE ANALYSIS AND DIAGNOSIS, MOLECULAR BIOLOGY AND “OMICS”
MULTIP
TIPAR
AROUS BUFF
UFFAL
ALOES SUBMITED TO EXOGENOUS MELATONIN IN LONG PHOTOPERIOD
OPERIOD
Abisai de Oliveira Sousa
UEMA/CCA/CMV, SAO LUIS, MA, BRAZIL.
This work objective was to evaluate the effect of melatonin subcutaneous implants containing 18 mg (Melovine) on reproductive
function of buffaloes during long photoperiod. The efficiency of treatments was tested evaluating estrus behavior, using teaser bulls with
reproductive device markers. The experiment included 100 females distributed in three groups: G1 = control group (n = 38), G2 = one melatonin
implant (n = 36) and G3 = three melatonin implants (n = 26). Eighty six (86%) females were in cyclical activities in the long photoperiod. They
were mated and 71 (82.6%) became pregnant. It was verified that 18 animals repeated heat totalizing 20 repetitions. Fourteen females stayed in
anestrous during all the experimental period, being 6 of group G1 (15.8%), 6 of group G2 (16.7%) and 2 of group G3 (7.7%). Only 32 (84.2%),
showed estrus in G1, and 10 (26.3%) were mating in the beginning and the end of the long photoperiod and 22 (57.9%) of them were during
the greatest luminosity. In the G2, 30 buffaloes were mating (83.3%), wherever 10 (27.8%) at beginning and the end of the long photoperiod and
the 20 remaining (55.6) during the greatest luminosity. And in the G3, 24 were mating (92.3%), but only 04 (15.4%) at beginning and the end
of the long photoperiod and the 20 remaining (76.9) were mating during the period of greatest luminosity. The data showed a service rate (cows
in heat/total number of cows) in G1=84.2%, G2=83.3% and G3=92.3% (P > 0.05), conception rate of: G1=78.1%, G2=83.3% and,
G3=87.5% (P > 0.05) and, pregnancy rate of: G1=65.8%, G2=69.4% and, G3=80.8% (P > 0.05). The results showed reproductive activity in
86% of buffaloes cows, with no differences (P > 0.05) between groups, indicating that reproductive behavior of this species prolongs almost
until photoperiod by 13 h approximately. Consequently, reproductive behavior was observed during the long photoperiod months, indicating a
pattern of reproductive behavior is different for this species. Moreover, the administration of melatonin exogenous did not affect the fertility
during this period.
Keywords: melatonin, seasonality, buffaloes.
A250 SUPPORTIVE BIOTECHNOLOGIES: CRYOPRESERVATION AND CRYOBIOLOGY, IMAGE ANALYSIS AND DIAGNOSIS, MOLECULAR BIOLOGY AND “OMICS”
EQUINE EPIDIDYMAL SEMEN FERTILITY AFTER THE ADDITION OF SEMINAL PLASMA
Cely Marini Melo e Oña, Luis Carlos Oña Magalhães, Frederico Ozanam Papa, Fernanda da Cruz Landim Alvarenga, Ian Martin,
Eduardo Gorzoni Fioratti & Marco Antonio Alvarenga
DRARV-UNESP, BOTUCATU, SP, BRAZIL.
Post-mortem recovery of viable spermatozoa from epididymides is a very important technique to obtain gene pool of genetically
valuable animals or even males of endangered species. Spermatozoa harvested from epididymides had no seminal plasma, which increased the
volume of the ejaculate and also contained substances that increase the sperm life inside the female genital tract. The aim of the present study was
to verify the influence that the addition of seminal plasma may have on equine epididymal sperm before freezing. Nineteen Brazilian Jumping
colts were castrated and their epididymis were stored for 24 h at 5 º C. The cauda epididymides were dissected and then flushed using 20 mL of
Botu-Semen ® then gathered into 5 pools before freezing: 4 pools composed of the sperm of 4 stallions and one pool composed of the sperm of
3 stallions. Each pool was divided into 2 samples and diluted 1:1 (v/v) with the following extenders: Botu-Semen ® (control) or seminal plasma
(Plasma) then incubated for 15 min, centrifuged at 600 x g for 10 min. The pellets were resuspended in Botu-Crio ® . Samples were cooled at 5ºC
for 20 min and frozen at 6 cm above the level of liquid nitrogen, for additional 20 min, then plunged into liquid nitrogen. The samples were thawed
at 46oC/20” and evaluated by computer-assisted semen motility analysis (CASA HTM IVOS 12). Twenty mares were used being randomly
inseminated with epididymal sample incubated with either Botu-Semen (control) or seminal plasma. The inseminations were performed 36 and
42 h after the induction of ovulation, using 400x10 6 total motile sperm, pre and post ovulation. The ovulation was induced using 1mg of
deslorelin acetate. Sperm parameters were analyzed by ANOVA (SAS, Institute, Inc., Cary, NC) followed by Tukey’s test to identify the
significant differences. Treatments were considered different if P < 0.05. Fertility rates were evaluated by X 2 (P < 0.05). Sperm parameters of
the samples incubated with Botu-Semen and Seminal Plasma were 44.6±13.2 vs. 46.2±12.9 and 19.2±5.7 vs. 17.7±5.1 for total motility and
progressive motility, respectively. Pregnancy rates were 80 and 50% for Botu-Semen ® and Seminal Plasma, respectively. Based on results of
the present study we can conclude that seminal plasma is not necessary to obtain pregnancy with equine epididymal sperm. [Financial
support: FAPESP].
Keywords: equine, epididymides, seminal plasma.
N
s461