2011 (SBTE) 25th Annual Meeting Proceedings - International ...

Acta Scientiae Veterinariae, 2011. 39(Suppl 1): Abstracts - 25th Annual Meeting SBTE-Brazil. August 2011.
A013 MALE REPRODUCTIVE PHYSIOLOGY AND SEMEN TECHNOLOGY
AGE AT PUBERTY IN BOARS SUBMITTED
TED TO PORCINE SOMATOTR
TROPIN (PST) TREATMENT
Camila Piz
izoni,
Cláudia F. Demar
emarco, Ismael Mateus Cavazini,
azini, Viviane Rohr
ohrig Rabassa
abassa, August
ugusto Schneider, Ivan Bianchi & Mar
arcio N. Cor
orrêa
rêa
UNIVERSIDADE FEDERAL DE PELOTAS, CAPÃO DO LEÃO, RS, BRAZIL.
Growth hormone (GH) or somatotropin (ST) is widely used in animal production. Several studies in humans relate the use of
exogenous GH to testicular development and age at puberty when there is a deficiency in endogenous GH production. In veterinary applications
GH use in healthy animals has also been investigated. The aim of this work was to evaluate the effect of exogenous pST administration on age
at puberty in healthy boars. Starting at 22 days of age, fifteen young boars were used (Landrace x Large White): 7 animals in Control Group, that
received 0.5 mL of saline solution each 3 days and 8 animals in GH Group, that received 90 µg/Kg IM of pST (Reporcin ® , Zamira Life Sciences
Pty Ltd., Australia) also each 3 days. The animals were trained to ejaculate for semen collection three times a week starting at 150 days of age.
For the training a dummy sow was used and the boars were allowed to ejaculate and semen was submitted to motility analysis (%). The animals
were considered as reaching puberty when motility was higher than 10%. For statistical analysis, means were compared by analysis of variance
with Tukey adjustment using the SAS software. Control Group reached puberty at 177.0 (± 3.1) days of age with 47.1% (± 11.2) of motility,
while GH Group reached puberty at 182.0 (± 2.0) days with 50.0% (± 8.0) motility. There was no difference for age at puberty (P = 0.19) and
motility in the first ejaculate (P = 0.83). In conclusion, growth hormone had no effect on age at puberty when injected in healthy prepuberal boars.
Keywords: puberty, boars, growth hormone.
A014 MALE REPRODUCTIVE PHYSIOLOGY AND SEMEN TECHNOLOGY
EVAL
ALUATION OF SPERM MEMBRANE INTEGRITY OF BUFF
UFFAL
ALOES (
(BUB
UBAL
ALUS BUB
UBALIS
ALIS) ) USING DIFFERENT
LABOR
ABORATORIAL
ORIAL TECHNIQUES
G. Rocha Silva 1 , Alexandre Rossetto Garcia 2 , Sâmia Rubielle Silva De Castro 3 , Benjamim de Souza Nahúm 2 , Alessandra Ximenes
Santos 1 , Arnaldo Algaranhar Gonçalves 1 & Daniel Vale Barros 4
1
UNIVERSIDADE FEDERAL DO PARÁ, BELEM, PA, BRAZIL. 2 EMBRAPA AMAZÔNIA ORIENTAL, BELÉM, PA, BRAZIL. 3 FACULDADES INTEGRADAS DO TAPAJÓS, SANTARÉM, PA, BRAZIL.
4
UNIVERSIDADE FEDERAL RURAL DA AMAZÔNIA, BELÉM, PA, BRAZIL.
Simple techniques, reliable and easy to execute are critical to laboratory analysis of semen. The assessment of integrity of sperm
membrane is an important element for andrology, because it allows evaluate the cell functionality, even when morphological defects are not
previously diagnosed. The eosin used in eosin-nigrosin (EN) seminal staining doesn’t penetrate the sperm presenting intact membrane.
However, when membrane are damaged, eosin stains sperm in pink, and nigrosin gives the contrast to visualize the unstained cells (Brito 2007, N
Clinical Technique Equine Practice 6, 249-264). The hypoosmotic swelling test (HOST) is able to evaluate the integrity of the cell membrane
considering the liquid transportation that occurs when membrane is intact. Consequently, tails become bent when the sperm cell is subjected to
hypoosmotic medium (Jeyendran et al., 1992, Archives of Andrology 29, 105-116). These research aimed was to compare the use of EN and
HOST in diagnosis of sperm membrane integrity on buffalo raw semen. The experiment was executed at Embrapa Eastern Amazon, in Belém,
Pará. Five buffalo bulls had semen collected by artificial vagina, and each bull provided six ejaculates (n = 30). Semen was assessed and each
ejaculate was subjected to EN and HOST tests HOST (Jeyendran et al. 1984, Journal of Reproduction and Fertility 70, 219-228). The plasma
membrane integrity (%) obtained with EN or HOST was correlated with progressive motility (%). Data were analyzed using t-Test and the level
of association between variables was calculated by Pearson correlation (P < 0.01). The percentage of spermatozoa with intact membrane detected
by the techniques EN and HOST was 85.4 ± 8.3% and 73.1 ± 10.5%, respectively (P < 0.01). Results were higher than those published by Iqbal
et al (2010, Journal of Animal Science 88, 922-925) using EN (69.1 ± 0.6%) and HOST (59.4 ± 0.8%) for analysis seminal of Nili-Ravi
buffaloes. The correlation of motility with EN was highly significant and of medium intensity (r=0.66, P < 0.0001), but it was not observed using
HOST (r = 0.19, P = 0.31). These findings indicate superior efficiency of EN technique for raw buffalo semen evaluation, when sperm motility
is used as a parameter for predicting the potential fertility of an ejaculate. Thus, it was concluded that EN and HOST techniques are reliable,
especially the first, which doesn’t require sophisticated equipment to be executed, such as phase-contrast microscope. Therefore, EN is
recommended for the analysis of the sperm membrane integrity of buffalo semen, that makes it profitable for laboratory routines.
Keywords: eosin-nigrosin, hypoosmotic swelling test, raw semen.
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