2011 (SBTE) 25th Annual Meeting Proceedings - International ...

Acta Scientiae Veterinariae, 2011. 39(Suppl 1): Abstracts - 25th Annual Meeting SBTE-Brazil. August 2011.
A155 OPU-IVP AND ET
OVULATOR
ORY RESPONSE OF CYCLING CLING MARES STIMULATED
TED WITH AN ASSOCIATION OF PFSH AND EPE
Natalia Claro de Abreu, Cezinande Meira , Fernanda Saules Ignácio & Gustavo Henrique Marques Araújo
UNESP, BOTUCATU, SP, BRAZIL.
It has been demonstrated by Krekeler et al., 2006 (Theriogenology, 66: Abstract No. 663) a satisfactory ovulatory response (2.28
ovulations/mare) after 25mg i.m. of porcine FSH (pFSH; Folltropin-V ® ) twice a day. This result was not confirmed by following studies
developed by Ignácio et al. in 2007 (Acta Scientiae Veterinariae 35, Supplement 3; Abstract:s1245) and 2008 (Acta Scientiae Veterinariae 36,
Supplement 2; Abstract:s504), in which a mean of 1.4±0.26 and 1.29±0.49 ovulations/mare, respectively, were found. Even though, treatment
contributed to an increased diameter of the second and third largest follicles. The present study aimed to reduce EPE length of treatment by
verifying the ovarian answer of mares treated with EPE after a pre-stimulation of pFSH. A total of 22 cross-bred mares were randomly assigned
into three groups: control (saline, n = 7), pFSH+EPE (n = 8) and EPE (n = 7). The hormonal treatment began at D7 (D0=ovulation) when
follicles =23mm were detected to allow beginning of treatment until deviation. Concomitantly, luteolysis was induced with 7.5mg of dinoprost
trometamine i.m. (Lutalyse ® ). In pFSH+EPE group, mares were treated with 25 mg i.m. BID of pFSH for four days followed by 12.5 mg i.m.
BID of EPE. In EPE group or control, 12.5mg of EPE or an equivalent volume of saline 0.9% were injected i.m. every 12h. In all groups,
treatments were maintained until the largest follicle achieved =35 mm, when ovulation was induced with hCG (Vetecor ® , 2500 UI, i.v.) followed
by two inseminations (1x109 viable sperm cells) 12 and 36h after induction. Transrectal ultrasound was done daily for follicles measurements
and ovulations detection. Analysis of variance followed by Student-Newman-Keuls test were used to compare number of follicles =25mm at
induction ovulation moment, number of ovulations and length of treatments. Number of follicles =25mm was higher (P < 0,05) for EPE group
(3.57±0.57) when compared to other groups and statistically similar between pFSH+EPE (2.0±0.38) and control (1.29±0.18), which reflected
over number of ovulations: 3.0±1.73, 1.5±0.76 and 1.0±0.0 ovulations/mare for EPE, pFSH+EPE and control groups , respectively. Treatment
length did not vary among groups but pFSH+EPE group needed less number of days of EPE (4.88±0.51) than EPE group (7.43±0.37).
According to results, it is concluded that pFSH treatment followed by EPE, even requiring a shorter EPE treatment time, was not enough to
significantly increase number of ovulations.
Keywords: superovulation, mares, reproduction.
A156 OPU-IVP AND ET
PREGNANCY RATE IN EQUINE EMBRYO RECIPIENTS TREATED
TED WITH FLUNIXIN MEGLUMINE
Jhonnatha Paulo Oliveira, , Hélène Lacerda de Resende & Júlio César Ferraz Jacob
UNIVERSIDADE FEDERAL RURAL DO RIO DE JANEIRO, SEROPEDICA, RJ, BRAZIL.
Is already known that fixation of the embryo into the uterus of recipient mares elicits a transient inflammatory response of the
endometrium. Although, in most cases this response remains subclinical, it may cause the release of prostaglandin (PGF2α) as part of an
inflammatory. The PGF2α acts luteolysis corpus luteum, causing death or expulsion of the transferred embryo before maternal recognition of
pregnancy. Non-steroidal anti-inflammatory drugs (NSAID) can then be used as anti-luteolytic. This study aimed to compare the rate of
embryonic fixation between recipients treated and untreated with Flunixin meglumine - Banamine ® (Intervet / Schering-Plough, Cruzeiro,
Brazil). Were performed 30 embryo transfers, in mares of Mangalarga Marchador breed, with age between four and 12 years, in the period from
January to April 2010, where 15 recipients were treated of randomly with 1,1 mg Banamine ® / kg intramuscular at the time of transfer and 15
recipients were not treated. We used only embryos considered as very good or excellent, with 8 or 9 days old, and synchronized recipients from
D0 to D+5 in relation to ovulation of the donor (D0 = day of ovulation). For comparison the pregnancy rate (PR) between groups was submitted
to the X2 test. Of embryo transfers performed, the PR was 64% (19/30). When comparing groups, the PR was 80% (12/15) between the
recipients treated and 46.5% (7/15) for recipients untreated (P= 0,05), showing that, in these conditions, the NSAID is viable and increases the
pregnancy rate.
Keywords: nsaid, pregnancy rate, mares.
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