2011 (SBTE) 25th Annual Meeting Proceedings - International ...

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Acta Scientiae Veterinariae, 2011. 39(Suppl 1): Abstracts - 25th Annual Meeting SBTE-Brazil. August 2011. A115 FTAI, FTET AND AI CONCEPTION RATES IN BEEF CATTLE TLE GIVEN A NEW OR PREVIOUSL VIOUSLY USED PROGESTER OGESTERONE ONE DEVICE IN FIXED-TIME ARTIFICIAL INSEMINATION Aline Kehrle 1 , Manoel Pereira Neto 2 , Milton Maturana Filho 1 , Patrícia Helena Paiva Miguez 1 , Gabriel Carvalho Gomes 1 , Orlando De Oliveira Junior 3 , Thiago Silva Antonio 3 & Ed Hoffman Madureira 1 1 UNIVERSIDADE DE SÃO PAULO, PIRASSUNUNGA, SP, BRAZIL. 2 FAZENDA GUARÁ, ALIANÇA DO TOCANTINS, TO, BRAZIL. 3 BIOGÉNESIS-BAGÓ, CURITIBA, PR, BRAZIL. Re-utilization of Progesterone (P4) inserts has been widely used, as the costs of a hormonal protocol can be reduced by device reutilization. An experiment was conducted aiming to compare FTAI conception rates (CR) in beef cattle given a new or previously used (Cronipres ® - Biogenesis-Bago, Argentina) P4 devices. In this experiment 763 lactating Nellore (Bos indicus) and crossbreed Simental x Nellore (Bos taurus x Bos indicus) cows with age ranged from 3-10 years, body condition score 4 to 7 (1-9 scale) were distributed in four FTAI groups. All animals were treated with 2 mg i.m. of Oestradiol Benzoate (Cronibest ® - Biogenesis-Bago, Argentina) and received the Cronipres ® P4 (containing 1 g of P4) device (CRO1 - new devices, n = 194; CRO2 – devices used once, n = 190; CRO3 – devices used two times and added with one P4 ring (0.1 g P4), n=191 and CRO4 – devices used three times and added with two more P4 rings, n = 188). Together with device withdrawal (day 8) all animals received 300 IU i.m. of eCG (Novormon ® , Schering–Plough, USA) and 150 µg i.m. of D–cloprostenol, (Croniben ® , Biogenesis-Bagó, Argentina). On day 9, all cows were treated with 1 mg of Oestradiol Benzoate (Cronibest ® - Biogenesis-Bago, Argentina) and FTAI was performed 30h later. In each group, treatments were equally distributed. The data were submitted to analysis of variance (PROC GLIMMIX – Statistical Analysis Sistem - SAS ® ), using as explanatory variables the effect of treatment (CRO1, CRO2, CRO3 e CRO4), body condition score (BCS5), ciclicity (cycling and non–cycling), FTAI groups (1 to 4) parity (5 mounths) and breed (Nellore and Simental X Nellore) and its interactions, eliminating the variables from the final model when P > 0.2. The final model included treatment (P = 0.18), breed (P = 0.11) and FTAI group (P = 0.14). CR did not differ among treatments (CRO1 – 55.1%a, CRO2 – 47.9%ab, CRO3 – 48.7%ab e CRO4 – 44.15%b) (P = 0.18). Overall CR was 49.1%. There was no statistical difference between treatments, however a larger number of animals should be tested. Keywords: previously used devices, ftai, progesterone. A116 FTAI, FTET AND AI USE OF DIB ® AND CRESTAR ® TO SYNCHRONIZE OVULATION AND FTAI IN BUFF UFFAL ALO HEIFERS DURING THE NON-BREEDING SEASON Nelcio Antonio Tonizza Car arvalho alho 1 , Júlia Gle leyci Soar oares 2 , Ever erton Luiz Reis 3 & Pietr ietro Sampaio Bar aruselli 2 1 APTA, REGISTRO, SP, BRAZIL. 2 VRA-FMVZ-USP, SÃO PAULO, SP, BRAZIL. 3 INTERVET/SHERING-PLOUGH, COTIA, SP, BRAZIL. The aim of this study was to evaluate the effect of intravaginal progesterone device (DIB ® ) and subcutaneous implant of progestogen (CRESTAR ® ) on follicular response and on conception rate in buffalo heifers synchronised to fixed time artificial insemination (FTAI) during the non-breeding season (spring and summer). For this, one hundred and third heifers (n = 130) were divided into two groups (DIB ® = GD, n = 65; CRESTAR ® = GC, n = 65) according to age, weight, body condition score and ovarian activity. One part of this animals (GD=13 and GC=17) were randomly selected to verify the follicular dynamics. At random stages of estrus cycle (Day 0 = D0; PM), buffaloes in GD received a DIB ® (1,0gr. of P4, Intervet/Schering-Plough, Brazil) and in GC a CRESTAR ® (3.0mg of Norgestomet, Intervet/Schering-Plough, Brazil). At the same moment (D0, PM), all animals received more 2.0mg of Estradiol Benzoate (i.m.; Gonadiol ® , Intervet/Schering-Plough, Brazil). On D9 (PM), the devices and implants were removed and was administrated 150µg of PGF2a analogue (i.m.; d-cloprostenol sodic, Preloban ® , Intervet/ Schering-Plough, Brazil) plus 400IU of eCG (i.m.; Folligon ® , Intervet/Schering-Plough, Brazil) in all buffaloes. After 48h (D11, PM), the ovulation was induced by 10µg of GnRH (i.m.; Buserilin Acetate, Conceptal ® , Intervet/Schering-Plough, Brazil). The AI was realised with applicator and sheath specific for sheep and goats (IMV, France) 16h after the last hormonal administration (D12, AM). The ultrasound (Mindray, DP2200Vet, China) examination was performed in all heifers on D0 to verify the ovarian status and on D42 – thirty days after the FTAI – to the pregnancy diagnosis. From D9 to D11 (24/24h) and from D11 to D14 (12/12h for 60h) the 30 heifers selected to verify the follicular dynamics was submitted to access the follicular diameter and the moment of ovulation by ultrasonography. Data were analyzed by ANOVA of the SAS for Windows program and by Chi-square test. There were no significant differences (P > 0.05) between groups GD and GC for the dominant follicle diameter (Æ) on D9 (1.0 ± 0.1 vs.. 1.0 ± 0.0 cm), the ovulatory follicle Æ (1.4 ± 0.0 vs.. 1.4 ± 0.0 cm), the interval GnRH administration/ovulation (29.0 ± 3.7 vs.. 30.0 ± 1.9 h), the ovulation rate [92.3% (12/13) vs.. 94.1% (16/17)] and to the conception rate [47.7% (34/65) vs.. 47,7% (34/65)]. Both hormonal protocols used at the present study provided satisfactory follicular response and conception rate in FTAI buffalo heifers during the non-breeding season. Keywords: ftai, buffalo heifers, non-breeding season. s394
Acta Scientiae Veterinariae, 2011. 39(Suppl 1): Abstracts - 25th Annual Meeting SBTE-Brazil. August 2011. A117 FTAI, FTET AND AI REPRODUCTIVE ULTR TRASONOGR ASONOGRAPHY APHY FOR USE OF HIGH COST SEMEN IN FTAI PROGR OGRAMS José Ribamar De Souza Tor orres Júnior, Hans Muller Vaz Braga Aguiar & Paulo Henr enrique Cavalc alcan anti UNIVERSIDADE FEDERAL DO MARANHÃO, CHAPADINHA, MA, BRAZIL. The objective was to evaluate the impact of reproductive ultrasonography based on costs of semen in FTAI programs. Lactating Nelore cows (n = 796), were allocated in different handled treatments according to use or not of reproductive ultrasound examination at FTAI (US vs. noUS) (US; CHISON D600VET, USPBrazil Eletromedicina, Brazil) and inseminated of two manners: aleatory or dominant follicle diameter dependent (DF = or < 11mm). We hypothesized that the biggest DF have best chances of increases ovulation and conception rates. The DF=11mm animals were inseminated with high cost semen (T35US; $35.00/batch) and the DF
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