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Acta Scientiae Veterinariae, 2011. 39(Suppl 1): Abstracts - 25th Annual Meeting SBTE-Brazil. August 2011. A071 FOLLICULOGENESIS, OOGENESIS AND SUPEROVULATION ENERGY SUPPLEMENTATION TION IN SANTA A INÊS SHEEP Sabr abrina Silv ilva Ven entur turi 1 , Isab sabel Roussouliér oussouliéres Soar oares 2 , Andr ndressa Fer erreir eira Da Silv ilva 3 , Elizab lizabeth Cruz Car ardoso 4 , André Rios Rodr drigues 5 , Rodolpho Almeida Tor orres Filho 6 , Jeferson Ferreira Fonseca 7 & Felipe Zandonadi 8 1,2,3,4,5,6,8 UNIVERSIDADE FEDERAL FLUMINENSE, NITEROI, RJ, BRAZIL. 7 EMBRAPA CAPRINOS E OVINOS, CORONEL PACHECO, MG, BRAZIL. Follicular growth is mainly controlled by gonadotropins and locally produced growth factors. However, many environmental factors such as nutrition can influence follicular development, oocyte quality, the rate of ovulation and consequently, fertility. Caloric supplementation causes an increase in circulating concentrations of glucose and insulin, leading to a rise in the number of follicles as well as an increase in follicular diameter and ovulation rate (SOMCHIT et al., 2007, Theriogenology 68, 1037-1046, VIÑOLES et al.,2009, Animal Reproduction Science 113, 82-92). This study aims to evaluate the effects of increasing energy by 20% through dietary supplementation as an induction procedure and synchronization of estrus in ewes on ovulation rate, follicle diameter and circulating concentrations of glucose and insulin. Forty-three Santa Inês sheep were chosen and separated into two experimental groups where the Gcontrol group received a maintenance diet and the GM+20% received a maintenance diet with a supplementation providing a 20% increase of energy during the induction period and synchronization of estrus. The GM+20% group underwent a 6-day procedure where an intravaginal sponge was inserted containing 60 mg of medroxiprogesterone acetate (Progespon ® , Shering Plough Saúde Animal, São Paulo, Brazil). There was administration of 37.5 µg of d-cloprostenol (Veteglan ® , Lab. Hertape-Calier Saúde Animal S/A, MG, Brazil) and 200 IU of eCG (equine chorionic gonadotropin - Novormon 5000 ® , Syntex Ind. Biochemistry, Buenos Aires, Argentina) 24 h before sponge removal. The monitoring of ovarian follicular dynamics was carried out to determine the timing and number of ovulations, and the diameter of preovulatory follicles. Ultrasound examinations were conducted to evaluate follicular diameter and ovulation rate. Bloods was collected to determine plasma insulin and glucose concentrations and were made with the animals before diet was offered. Statistical analysis was performed through Duncan´s Test. In relation to plasma insulin concentration, it was observed that the GM+20% presented with high concentrations (1,66 ± 1,51 vs. 3,04 ± 3,66 (ng/mL) – P > 0,05). Plasma glucose concentrations did not change (70,20 ± 7,72 vs. 69,70 ± 25,46 – (mg/dL) P > 0,05). The results showed no differences in follicular diameter (6,00±0,20 vs. 5,90±0,60- P > 0,05) or in the rate of ovulation (1,00 vs. 1,13 - P > 0,05) between the groups. Therefore the supplementation addressed in this study led to an increase in insulin, however was not sufficient to affect reproductive parameters and plasma glucose concentration. Keywords: sheep, supplementation, santa inês. A072 FOLLICULOGENESIS, OOGENESIS AND SUPEROVULATION VARIA ARIATION ON VASCUL ASCULARIZA ARIZATION OF FOLLICLES INDUCED TO OVULATE WITH HCG AND GNRH EVAL ALUATED BY DOPPLER ULTR TRASONOGR ASONOGRAPHY APHY - PRELIMINARY RESULTS Renata Cristina Uliani 1 , Luciano Andrade Silva 2 & Marco Antonio Alvarenga 3 1,3 FMVZ UNESP, BOTUCATU, SP, BRAZIL. 2 FZEA USP, PIRASSUNUNGA, SP, BRAZIL. Only few studies are avaliable in the literature with use of Doppler in equine reproduction which has been used for study of vascularization of preovulatory follicle, follicular and oocyte quality check, vascularization of corpus luteum and uterus, establishment of pregnancy and evaluation of pregnancy. Recent studies with Doppler have shown changes in blood flow and vascularization of the follicular wall at the time close to ovulation. For this reason Doppler transrectal ultrasonography has been used in studies of ovarian and follicle hemodynamics in large animals. Ovulation is an outstanding event in the study of reproduction, which makes its induction a biotechnology of reproduction that deserves special attention. The products commercially available for ovulation induction are hCG and deslorelin, a GnRH analogue. HCG is a protein hormone with LH action. Its composition is similar to equine LH but with a half life much longer because contains much sialic acid. The deslorelin administered intramuscularly induces the release of endogenous LH by the mare. Objectives: Is absent in the literature studies concerned in compare the follicular vascularization after administration of hCG and GnRH, thus, the objective of this work was to check the variation on vascularization of the follicle after the ovulation induction with each one of the inductors. Material and methods: Two estrous cycles of five mares (
Acta Scientiae Veterinariae, 2011. 39(Suppl 1): Abstracts - 25th Annual Meeting SBTE-Brazil. August 2011. A073 FOLLICULOGENESIS, OOGENESIS AND SUPEROVULATION VIABILITY OF CANINE PREANTRAL AL FOLLICLES CULTURED IN VITRO AFTER CONSER ONSERVATION IN AN OOCYTE TRANSPOR ANSPORTER Rodrigo José Sousa Gonçalves 1 , Vanessa Raquel Pinto Barros 2 , Anderson Pinto Almeida 3 , Ana Beatriz Graça Duarte 4 , Valdevane Rocha Araújo 5 , Cleidson Mano anoel Gomes Silv ilva 6 , Gio iovanna Quin uintino Rodr drigues 7 , Ger erlane Modest desto Da Silv ilva 8 , José Ric icar ardo De Figueir igueiredo edo 9 & Mar aria Helena Tavar ares Matos 10 1,2,10 UNIVERSIDADE FEDERAL DO VALE DO SÃO FRANCISCO, PETROLINA, PE, BRAZIL. 3,4,5,6,7,8,9 UNIVERSIDADE ESTADUAL DO CEARÁ, FORTALEZA, CE, BRAZIL. Maintenance of follicular quality after collection and transportation of the ovaries to the laboratories of reproductive techniques is a limiting factor for the success of in vitro culture of preantral follicles (PAF) in the canine species. In this way, the aim of this study was to assess the viability of canine PAF cultured in vitro after storage for 12 or 24 h in an oocyte transporter ® - Compact, Wta-Brazil (TO). After collection of canine ovaries,180 secondary preantral follicles with a diameter higher than 200 mm were mechanically isolated, and destined to conservation into the oocyte transporter at 39°C in α-Minimum Essential Medium supplemented with BSA (3 mg/mL), glutamine (2 mM), hypoxanthine (2 mM), ITS (insulin 10 µg/mL, transferrin 5.5 µg/mL, and selenium 5 ng/mL), ascorbic acid (50 ag/ml) and recombinant FSH (100 ng/ml). The PAF were further destined to in vitro culture using the same conservation medium. Six treatments were used: PAF assessed by fluorescence microscopy (FM) after isolation (T1), PAF cultured after isolation (T2 - cultured control), PAF destined to (FM) after 12 (T3) or 24 h (T4) of conservation, PAF destined for the culture after 12 (T5) or 24 h (T6) of conservation. PAF were individually cultured in 100 mL drops of medium, at 39°C and 5% CO2 for 7 days. Every 2 days, 60 ìl of the culture medium was changed by a fresh medium. The follicular viability was analyzed using the fluorescent markers calcein-AM (4µM) and ethidium homodimer-1 (2µM) for viable and nonviable PAF, respectively. At the end of the isolation, preservation in the TO and the culture for 7 days, PAF of all the treatments (100%) analyzed by FM were stained in green by calcein-AM demonstrating that all them are viable the that explains the absence of statistical analysis of this experiment. In conclusion, canine preantral follicle preserved for 12 and 24 h in an oocyte transporter maintain the viability in vitro after culture for 7 days. Keywords: canine, preantral follicle, preservation. A074 FOLLICULOGENESIS, OOGENESIS AND SUPEROVULATION ASCORBIC ACID IMPROVES THE SURVIV VIVAL AND IN VITRO GROWTH OF ISOLATED CAPRINE PREANTRAL AL FOLLICLES Gerlane Modesto da Silva 1 , Carlos Henrique Lobo 2 , Valdevane Rocha Araújo 1 , Ana Beatriz Graça Duarte 1 , Arlindo Alencar Moura 2 & José Ricardo de Figueiredo 1 1 UECE, FORTALEZA, CE, BRAZIL. 2 UFC, FORTALEZA, CE, BRAZIL. Ascorbic acid (AA) may act to regulate the matrix metalloproteinases and their inhibitors associated with the turnover of collagen on the basement membrane during follicle growth (Murray et al., 2001; Journals of Reproduction and Fertility 121, 89-96). The present study aims to investigate the influence of AA on the survival, growth, antral formation, extruded oocytes and mRNA expression of the matrix metalloproteinases-9 (MMP9) and their tissue inhibitor-2 (TIMP2) on caprine preantral follicles (FOPA). Isolated FOPA were individually cultured without (MEM+) or with AA at 50 µg/mL (AA50) or 100 µg/mL (AA100) during 18 days. To culture at least 32 follicles were used per treatment. Three pools of 10 follicles from non cultured (fresh control), MEM+ alone, AA50 and AA100 were collected to quantified the genes MMP9 and TIMP2 by real-time polymerase chain reaction (qPCR). The percentage of survival, antrum formation, extrusion rate and maturation were compared by x2 test (StatView for Windows). The diameter and growth rates were compared by Kruskal-Wallis test. For qPCR differences between the control and treatments was assessed with the T test of SAS 9.0. At the end of culture, AA50 significantly increased the percentage of follicular survival (59.38% vs. MEM+: 29.03%; AA100: 44.83%; P < 0.05) and antral formation (65.63% vs. MEM+: 34.78%; AA100: 52.00%; P < 0.05) compared with MEM+ alone. After 6 days of culture, AA50 (319.14±89.70) increased follicular diameter (mm), compared with the other treatments (MEM+: 229.59±78.13; AA100: 269.80±68.50; P < 0.05). At day 12, both concentrations of AA (AA50: 452.38±136.81; AA100: 451.98±165.45) increased follicular diameter when compared with control medium (242.11±131.69; P < 0.05). Moreover, mean increase in follicular diameter daily (µm/day) was higher in the presence of both concentrations of AA (AA50: 18.15±10.57 and AA100: 20.94±10.43) than in MEM+ alone (11.38±9.46; P < 0.05). Lower percentages of extruded oocytes were observed in medium containing AA50 (18.75%) compared with control medium (56.52%); P < 0.05. With respect to the maturation, all fully grown oocytes showed an intact germinal vesicle. The qPCR assays showed that AA50 show mRNA expression for MMP9 was higher 2.4 times when compared to MEM+ alone (P < 0.05). In addition, mRNA expression for TIMP2 gene was higher 2.08 times in AA100 than in MEM+ (P < 0.05). In conclusion, AA at 50 µg/ml increase the caprine FOPA viability and development after in vitro culture and influences the enzyme MMP9 involved with basement membrane remodeling. Keywords: mmp9, timp2, ascorbic acid. N s373
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R.B. Lôbo, D. Nkr uman, D.A. Gross
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M.E.F .F. Oliv liveir eira. 2011. E
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A.L. Gusmão usmão. 2011. Estado d
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J. R. Figueir igueiredo edo, A.P.R.
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E.L.A. Motta, M. Nichi & P.C. Ser e
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D.P .P.A.F .A.F. Braga & E. Bor org
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R.C. Uliani, L.A. Silv ilva, M.A. A
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F.S. Ignácio, J.C. Fer erreir eira
Page 142 and 143:
F.S. Ignácio, J.C. Fer erreir eira
Page 145 and 146:
L.A. Silva. 2011. Local Effect of t
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M.M. Franc anco, A. Pellegr ellegri
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M.M. Franc anco, A. Pellegr ellegri
Page 161 and 162:
B. Str troud & G.A. Bó. 2011. The
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W.W .W. Tha hatcher cher. 2011. Tem
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O. Sandra. 2011. Deciphering early
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R.C. Cheb hebel. el. 2011. Use of A
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A.C.A. Net eto, A.R. Galdos aldos,
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A.C.A. Net eto, A.R. Galdos aldos,
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P.C .Cha havett ette-P e-Palmer alm
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E.H. Bir irgel Junior unior, F.V .V
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P. Humblot. 2011. Reproductive Tech
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J.A. Piedrahita. 2011. Application
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O.E. Smith, B.D. Murphy & L.C. Smit
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D. Salamone alamone, R. Bevacqua, F
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E.A. Maga & J.D. Mur urray. 2011. G
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C.G. Gutier utierrez, S. Fer errar
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B. Gasparrini. 2011. Ovum pick-up a
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B. Gasparrini. 2011. Ovum pick-up a
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