2011 (SBTE) 25th Annual Meeting Proceedings - International ...

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Acta Scientiae Veterinariae, 2011. 39(Suppl 1): Abstracts - 25th Annual Meeting SBTE-Brazil. August 2011. A99 FTAI, FTET AND AI EFICIENCY OF TIMED ARTIFICIAL INSEMINATION PROTOC OCOL OL FOR HOLSTEIN COWS REARED UNDER SEMIARID CONDITION Sebastião Inocêncio Guido 1 , Antonio Santana Dos Santos Filho 2 , Lucas Carvalho Pereira 3 , Joaquim Corrêa De Oliveira Andrade 4 , Fabiani Coutinho Lordão Guido 5 & Claudio Coutinho Bartolomeu 6 1,2,3,4 INSTITUTO AGRÔNOMICO DE PERNAMBUCO - IPA, RECIFE, PE, BRAZIL. 3,4,5 VETERINÁRIO AUTONÔMO, MACEIÓ, AL, BRAZIL. 4,5,6 VETERINÁRIO AUTONÔMO, RECIFE, PE, BRAZIL. 6 UFRPE, RECIFE, PE, BRAZIL. This study was conducted at the Experimental Station of São Bento do Una- (ESSBU/IPA ) Pernambuco, Brazil, (Latitude 08 31' 35’’ and Longitude 036 27’ 34.8'’) with the aim to evaluate the efficiency of protocol for TAI in dairy cows reared in semi-arid conditions during the dry season (relative humidity of 53.5% and mean average environment temperature of 25.3°C). There were used lactating cows with over 100 days of lactation with an average milk production of 7,200 kg/cow/lactation in 305 days and age ranging from 36 to 108 months. The herd was kept in semi-intensive system, receiving a diet composed of cactus pear (Opuntia ficus indica, Mill), sorghum silage (Sorghum bicolor (L.) Moench) and concentrated with 18% crude protein (CP), and a mineral supplement and water ad libitum. All cows in the voluntary waiting period (VWP) were submitted to gynecological examination by rectal palpation, being distributed in two treatments T1 and T2: T1 (n = 30) was composed of cows with palpable CL which received an application of 0.530mg of cloprostenol, and were artificial inseminated 12 h after being observed in estrus. At T2 (n = 28) cows with no palpable CL were submitted to TAI. The protocol consisted of an intravaginal progesterone device inserted at D0 along with 2mg of estradiol benzoate (EB), at D7 application of 0.530mg cloprostenol and 300 IU of eCG, at D8 device removal and at D9 application of 1 mg EB. Timed artificial Insemination was performed 54h after device removal at D10. Data were subjected to analysis of variance and means were compared by Tukey test at 5% by the statistical package SPSS 16 for windows. The cows on T2 underwent ultrasound evaluation of the ovulatory response during estrus at the time of EB application. All cows in both groups T1 and T2 were artificial inseminated with conventional semen. Pregnancy diagnosis was performed by rectal palpation aided by ultrasound in the 45th day after AI. The conception rate (CR) was 26.66% (8/30) in T1 and 25.00% (7/28) in T2, (P > 0.05). Regarding to the occurrence of estrus in cows from T1, it ranged from 48 to 72 h after application of PGF2a. In T2 follicular diameter measured at D9 ranged from 10.9 to 25.4mm. In the conditions of this experiment, the protocols used showed low CR. However the treatment used in the T1 has a better cost-benefit. Keywords: tai, bovine, holstein. A100 FTAI, FTET AND AI LUTEOL UTEOLY TIC EFECTIVENESS OF PROST OSTAGL GLANDINA TOR ORTUGA ® IN BOVINE CAT TLE M arc os Sampaio Bar aruselli 1 , Rob ober erta Machado Fer err eira 2 , Mano anoel Fr ancisco Sá Filho 3 , Luis Fer ernando Mon ont eiro Tamassia 4 , Camila Alves Nascimento 5 , Renan Oliveira Fernades 6 & Pietro Sampaio Baruselli 7 1,4,5,6 TORTUGA CIA ZOOTECNICA AGRARIA, SÃO PAULO, SP, BRAZIL. 2,3,4,5,6,7 FMVZ-USP - DEPARTAMENTO DE REPRODUÇÃO ANIMAL, SÃO PAULO, SP, BRAZIL. The aim of the present study was to evaluate the effect of the administration of 0.150 mg of Prostaglandina (PGF) Tortuga ® , D (+) Cloprostenol, on (1) the morphological regression of the corpus luteum (CL) and (2) the reduction of serum progesterone (P4) concentration on days 8 and 12 of the estrous cycle in Nelore (Bos indicus) heifer. Pubertal heifers were homogenously allocated in one of Five experimental groups to receive none PGF (Control; n = 11), PGF Tortuga ® on the 8th (n = 12) or 12th (n = 14) day of the estrous cycle, or Ciosin ® on 8th (n = 14) or 12th (n = 12) day of the estrous cycle. At random stages of the estrous cycle, all heifers were treated with a norgestomet ear implant (Crestar ® , Intervet, Brazil) and 2 mg of estradiol benzoate (Gonadiol ® , Intervet, Brazil). After eight days, the implant was removed and 1 mg of estradiol cypionate (E.C.P. ® , Pfizer, Brazil) plus 0.530 mg of sodic cloprostenol (Ciosin ® , Intervet, Brazil) were administered. Morphological and functional regression of the CL was evaluated by ultrassonography and P4 concentration, respectively. Daily ultrassonographic exams were done for four days following PGF administration. Blood samples were collect right before PGF administration (0h) and then 6, 12, 24, 48, 72 and 96 h after treatment. P4 concentration was determined by radioimmunoassay (Coat-A-Count ® , Siemens, USA). Luteolysis rate (%; heifers reaching serum P4 concentration below 1ng/ml after treatment / all heifers treated) was analyzed using test FREQ of SAS for Windows. Progesterone concentration was analyzed using PROC GLIMMIX from SAS. No interaction was found among treatment and day of treatment (8th or 12th day of the estrus cycle), thus data was grouped. Luteolysis rate (%) was greater (P < 0.01) when heifers were treated with P4 Tortuga ® and Ciosin ® than Control heifers (88.5% vs. 80.8% vs. 0.0%, respectively). Morphological regression of the CL was evidenced in both, heifers treated with PGF Tortuga ® (4,283.0 ± 1,849.3 mm3 before treatment and 915.2 ± 880.2 after 96h) and treated with Ciosin ® (4,074.0 ± 1,746.7 mm 3 and 715.5 ± 588.6 mm3, respectively). Similarly, serum P4 concentration was reduced (P < 0.05) after treatment with both, PGF Tortuga ® and Ciosin ® , respectively (0h: 3.4 ± 1.7 vs. 4.3 ± 1.9 ng/mL; 96 h: 0.18 ± 0.20 vs. 0.25 ± 0.31 ng/mL). There was no difference (P > 0.05) on the CL regression and the reduction of P4 concentration between heifers treated with PGF Tortuga ® and Ciosin ® . In conclusion, the administration of 0,150 mg of PGF Tortuga ® efficiently promotes luteolysis in heifers, leading to morphological and functional regression of the CL. Keywords: bovines, protaglandin, luteolysis. s386
Acta Scientiae Veterinariae, 2011. 39(Suppl 1): Abstracts - 25th Annual Meeting SBTE-Brazil. August 2011. A101 FTAI, FTET AND AI ESTABLISHMENT OF A SYNCHRONIZA ONIZATION OF OVULATION PROTOC OCOL OL FOR FIXED TIME EMBRYO TRANSFER APLLIED ON DAIRY CATTLE TLE GENETIC IMPROVEMENT PROGR OGRAM OF ACRE STATE TE Rodolpho Satrapa 1 , Jefferson Viana Alves Diniz 2 , Francisco Lopes Dantas 2 , Rosano Ramos Marcelino 2 , Neilton Lima Vasconcelos 2 , Marcos Nereu Luckner 2 , José Marques Carneiro Junior 3 & Rafael Augusto Satrapa 4 1 UFAC, RIO BRANCO, AC, BRAZIL. 2 SECRETARIA DE ESTADO DE AGROPECUARIA, RIO BRANCO, AC, BRAZIL. 3 EMBRAPA, RIO BRANCO, AC, BRAZIL. 4 UNESP, SAO PAULO, SP, BRAZIL. In Acre State, the low technological level of the milk-producing farms, mostly with crossbred zebu-crossed and low genetic pattern, with average production of around 3 to 5 kg / day during the local rainy season has prompted the Acre Agriculture State Department to implement a genetic improvement program for dairy cattle based on the technique of embryo transfer (ET), an important tool that facilitates the rapid multiplication of animals of high genetic potential. The program also aims social gains, since an increase in productivity of livestock, may be reflected in improved economic conditions of farmers. For this purpose, the Improvement and Dissemination of Animal Genetics Station (EMDGA) was installed and equipped with a laboratory for embryo production by superovulation or IVF procedures from Gyr donors and sexed semen from Holstein bulls. With this purpose, a synchronization of ovulation (Ovs.ynch) protocol of recipients for FTET was established, which, logistically and economically, best suited with the distances to be traveled to the properties, many of them inaccessible due to the characteristics of the region. In 30 properties we evaluated 525 crossbred cows with varying degrees of blood (Bos taurus x Bos indicus), between 3 and 6 years of age and calved between 60 and 90 days. Before the start of treatment 197 cows (197/525 = 37.52%) were rejected because of their poor body condition score (BCS) ( / = 18mm by U.S. (Aloka SSD 500, Aloka, Japan) each of 235 cows (235/328 = 71.65%) received one fresh embryo (morula or blastocyst stage, grade 1 or 2); 93 cows (93/235 = 28.35%) were rejected by BCS
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