2011 (SBTE) 25th Annual Meeting Proceedings - International ...

Acta Scientiae Veterinariae, 2011. 39(Suppl 1): Abstracts - 25th Annual Meeting SBTE-Brazil. August 2011.
A107 FTAI, FTET AND AI
OVARIAN PAR
ARAMETERS AMETERS OF DAIRY GOATS UNDERGOING SYNCHRONIZA
ONIZATION
WITH PGF2Á ASSOCIATED OR NOT WITH THE
USE OF HCG IN THE BEGINNING OF ESTRUS
Luciana Vieira Esteves, , Felipe Zandonadi, Andressa Ferreira Da Silva, Sabrina Silva Venturi & Jeferson Ferreira Fonseca
UFF, NITEROI, RJ, BRAZIL.
The estrous synchronization refers to the concentration of the occurrence of estrus in restricted period (24 to 72 h) during the
breeding season. The aim of present study was to evaluate the ovarian responses after estrous synchronization protocol using two doses of
prostaglandin associated of use of hCG at onset of estrus in dairy goats. A total of 29 females were used (n = 10 Toggemburg, n = 10 Alpine and
n = 9 Saanen breeds). The females were assigned into two experimental groups according the use of 250IU hCG at onset of estrus (T1= Control;
1 mL of saline solution and T2=hCG). Both groups received two doses of 30µg d-cloprostenol via submucosa vulvar with 10 days apart.
Ultrasonography exams were performed twice daily after the second dose of PGF2α. All females were inseminated 16 h after onset of estrus.
All animals that did not display estrus (20.7%; 6/29) after the second dose of PGF2α did not ovulate. However, all females that displayed estrus
ovulated. There is a higher incidence of ovulation during the night period 69.6% (16/23) than during the diurnal period 30.4% (7 / 23). The
intervals from the second application of PGF2α to the occurrence of ovulation (h) and from onset of estrus to ovulation (h) did not differ
between treatments groups (P > 0.05), 85.7 ± 10.4 h vs. 87.5 ± 12.8 h, 41.0 ± 13.2 h vs. 38.8 ± 11.9 h, respectively for T1 and T2. The
diameter of the largest follicle and the second largest follicle (mm) and number of ovulations did not also different between treatments (P >
0.05), 6.8 ± 0.5 vs. 7.7 ± 1.9, 6 , 2 ± 0.5 vs. 6.9 ± 1.7, 1.8 ± 0.6 vs.. 1.8 ± 0.6, respectively. The conception rates did not differ (P > 0.05) among
females that did receiving (T2= 54.4%; 6/11) or not hCG (T1= 66.7%; 8/12). The results of the present study demonstrated that the estrus
of the dairy goats can be efficiently synchronized through the administration of two doses of PGF2α 10 days apart. Also the administration
of hCG at the time of onset of estrus did not affect the results.
Keywords: synchronization, goat, estrus.
A108 FTAI, FTET AND AI
THE PROTOC
OCOL OL FOR FTAI
WITH ESTRADIOL CYPIONATE DOES NOT REQUIRE ADITTIONAL TIONAL GNRH AND ALLOWS
INSEMINATION IN TWO PERIODS
Felip
elipe e Pitanga
Tor
orres
1 , Rob
ober
erta Machado Fer
erreir
eira 2 , Flávio Aragon Lima 3 , Márcio Bar
arciela
Ver
eras
4 , Felip
elipe e Rangel França Silv
ilva 5 , Thiago Guz
uzella Guida
6 , Rodr
drigo
Vasconcellos Sala 7 & Pietro Sampaio Baruselli 8
1,3,4,5,6
POLICLINICA VETERINÁRIA PIONEIROS LTDA, CARAMBEÍ, PR, BRAZIL. 2,7,8 FMVZ-USP, SÃO PAULO, SP, BRAZIL. 5 UENP, BANDEIRANTES, PR, BRAZIL. 6 UNESP, BOTUCATU, SP,
BRAZIL.
The aim of the present study was to evaluate time to insemination and the necessity of administering GnRH in high-producing
Holstein cows treated for fixed time artificial insemination (FTAI). The study was conducted in ten commercial dairy farm located around
Carambeí city, state of Paraná, Brazil. A total of 424 Holstein cows received 2mg estradiol benzoate (Gonadiol ® , Intervet, Brazil) and one
progesterone-releasing intravaginal device (CIDR ® , Pfizer, Brazil) on Day 0 (D0). On D8, device was removed and all animals received 25mg
dinoprost (Lutalyse ® , Pfizer, Brazil) and 1 mg of estradiol cypionate (E.C.P. ® , Pfizer, Brazil). Then, cows were homogenously allocated to one
of four experimental groups accordingly with time to insemination and the use of GnRH. (Fertagyl ® , Intervet, Brazil):1) AI 48h after device
removal and without concurrent the administration of GnRH, 2) AI 48h with GnRH, 3) AI 56h without GnRH, 4) AI 56h with GnRH. Statistical
analysis was done using PROC GLIMMIX from SAS. No interactions were observed among time to insemination and treatment with GnRH.
Thus, data was grouped. Similar P/AI 30 [without GnRH = 37.3% (n = 212) vs. with GnRH = 41.5% (n = 212); P = 0.28] and 60 days after
FTAI [without GnRH = 33.0% (n = 212) vs. with GnRH = 35.9% (n = 212); P = 0.61] was observed between cows treated or not with GnRH.
Pregnancy loss between 30 and 60 days after FTAI was also similar among groups [without GnRH = 11.4% (n = 79) vs. with GnRH = 13.6%
(n = 88); P = 0.16]. This protocol allowed the performance of AI in both periods 48 (AM) and 56 h (PM) following device removal, without
compromising pregnancy rates 30 [48h = 40.3% (n = 216) vs. 56h = 38.5% (n = 208); P = 0.63] and 60 days [48h = 33.8 (n = 216) vs. 56h =
35.1% (n = 208); P = 0.72] after FTAI. Pregnancy loss was also unaffected by time to AI [48h = 16.1 (n = 87) vs. 56h = 8.8% (n = 80); P =
0.67]. The results are suggestive that the administration of additional GnRH at FTAI in not necessary when estradiol cypionate is used at device
removal. Besides, the protocol used herein allows the performance of AI both in the morning (48h) and in the afternoon (56h) enabling the
synchronization of large number of animals (same protocol with different time to AI and similar efficiency). [Acknowledgments: Chaparral,
Erica, Frizia, Gruno, IMKJE, Ipê, São Cristóvão, Tainha, Três Lagoas, Pfizer Saúde Animal].
Keywords: bovine, estradiol cypionate, gnrh.
s390