2011 (SBTE) 25th Annual Meeting Proceedings - International ...

Acta Scientiae Veterinariae, 2011. 39(Suppl 1): Abstracts - 25th Annual Meeting SBTE-Brazil. August 2011.
A111 FTAI, FTET AND AI
SYNCHRONIZA
ONIZATION AND INDUCTION OF FERTILE ESTRUS OF PACA A (
(CUNICUL
CUNICULUS US PACA A L.)
Vânia Maria França Ribeiro 1 , Rodolpho Satrapa 2 , Jefferson Viana Alves Diniz 3 , Rafael Augusto Satrapa 4 & Rodolfo Rumpf 5
1,2
UNIVERSIDADE FEDERAL DO ACRE (UFAC), RIO BRANCO, AC, BRAZIL. 3 SECRETARIA DE ESTADO DE AGROPECUÁRIA DEPARTAMENTO DE MELHORAMENTO E DIFUSÃO
GENÉTICA DO ESTADO, RIO BRANCO, AC, BRAZIL. 4 DEPARTAMENTO DE FARMACOLOGIA, UNESP/BOTUCATU, SAO PAULO, SP, BRAZIL. 5 EMPRESA BRASILEIRA DE PESQUISA
AGROPECUÁRIA/CENARGEN, BRASILIA, DF, BRAZIL.
To verify the effect of progestin implants associated with two different dosis of gonadotropin on induction of estrus synchronization
and fertile on paca, 18 females composed the G1, G2 and G3, with six animals each. In females of G1 and G2 were inserted 1.5 mg norgestomet
(Crestar, Intervet Shering Plough), which were removed after eight days (D8), and 0.25 IU and 0.50 IU of eCG (Novormon 5000, Intervet
Shering Plough), taught in G1 and G2 respectively. Twenty-four hours before the D8, females received 0.5 mL of prostaglandin (Ciosin, Intervet
Shering Plough). G3 did not receive treatment and was paired with males on the same day that G1 and G2. G1 females presented estrus five,
seven, 10, 11 (paca /day) and 14 (two pacas/day) days and the G2 three (two pacas/day), eight, 11 (three pacas/day) days after D8, respectively.
G3 came in heat one, five, seven, eight, nine and 13 days after pairing with males. G1, G2 and G3 pregnancy rates were 100% (6 females), 66%
(4 females) and 50% (3 females), respectively. G1 and G2 and G3 calving rates were 83.3% and 100%, respectively. With respect of litter per
offspring, 100% of G1 and G3 produced one, while 50% of G2 produced two. The hormonal protocols used were effective in inducing and
synchronizing fertile estrus and improved the reproductive efficiency of pacas. [Financial support: Acre State Department of Agriculture].
Keywords: estrus synchronization, cuniculus paca l., progesterone.
A112 FTAI, FTET AND AI
SUPPLEMENTATION TION OF NELORE COWS WITH RUMEN-PR
UMEN-PROTECTED FAT BEFORE AND/OR AFTER TAI
Igor Miglioli Sokoloski 1 , Monique Mendes Guardieiro 2 , Alexandre Barbieri Prata 3 , Luis Henrique Dantas Carrijo 4 , Gerson Barreto
Mourão 5 & Roberto Sartori 6
1,2,3,5,6
ESALQ, PIRACICABA, SP, BRAZIL. 4 INTEGRAL NUTRIÇÃO ANIMAL, GOIÂNIA, GO, BRAZIL.
Previous studies have shown an increase in conception rate of Nellore cows supplemented with rumen-protected fat rich in linoleic
acid, from the beginning of the ovulation synchronization protocol or immediately after TAI (Lopes et al. 2009, J Anim Sci 87:3935-3943). In
contrast, supplementation with the same product previously to AI in Nellore embryo donors, have compromised in vitro embryo development
after cryopreservation (Guardieiro et al. 2010, Reprod Fertil Dev 22:205-206). Furthermore, in vitro supplementation with linoleic acid inhibited
oocyte development and maturation (Marei et al. 2010, Reproduction 139:979-988). Based on these contradictory results, the objective was to
evaluate the effect of supplementation of cows with rumen-protected fat before and/or after TAI on conception rate. We hypothesized that fat
supplementation before TAI compromises conception rates, whereas after TAI it increases. A total of 399 primiparous and multiparous Nellore
cows (60-80 days postpartum) with a mean BCS of 4 (scale of 1 to 9) were kept on pasture and randomly divided into four treatments according
to the daily supplementation: 100 g-Megalac-E ® (Arm & Hammer Church & Dwight Company, Brazil) per cow for 30 days before TAI (30B,
n = 98); for 30 days after TAI (30A, n = 101); for 30 days before and 30 days after TAI (60d, n = 100) or without Megalac-E ® (0d; Megalac-
E ® was replaced by kaolin, n = 100). Megalac-E ® was offered to cows as part of a supplement called Fertigral 200 ® (Integral Produbon Nutrição
Animal, Brazil). Cows were inseminated using the following ovulation synchronization protocol: D0 - CIDR ® (progesterone intravaginal
device, Pfizer Animal Health, Brazil) and 2.0 mg estradiol benzoate (Estrogin ® , i.m., Farmavet, Brazil), D6 - 12.5 mg dinoprost tromethamine
(Lutalyse ® , i.m., Pfizer Animal Health), D8 - 0.5 mg estradiol cypionate (ECP ® , i.m., Pfizer Animal Health), 300 IU eCG (Novormon 5000 ® ,
i.m., Intervet Schering-Plough, Brazil) and CIDR ® removal; D10 - AI. Pregnancy diagnosis was performed by ultrasonography 45 days after
TAI. The results were analyzed by the GLIMMIX procedure of SAS ® and are presented as least squares means ± SE. There was no difference
in conception rates at 45 days among groups (30B: 55.6 ± 6.7; 30A: 53.7 ± 6.6; 60d: 56.7 ± 6.6 and 0d: 49.9 ± 7.4%; P = 0.90), independently
of animal category. Contrary to our hypothesis, supplementation with rumen-protected fat rich in polyunsaturated fatty acids both before and/or
after TAI did not affect conception rate in postpartum Nellore cows. [Acknowledgments: To CNPq, FAPESP, Integral Produbon, Agropecuária
Vale do Sonho, Arm & Hammer and EMBRAPA (Innovation Network on Animal Reproduction - 01.07.01.002)].
Keywords: polyunsaturated fatty acids, reproduction, tai.
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