2011 (SBTE) 25th Annual Meeting Proceedings - International ...

Acta Scientiae Veterinariae, 2011. 39(Suppl 1): Abstracts - 25th Annual Meeting SBTE-Brazil. August 2011.
A131 OPU-IVP AND ET
THE EFFECT OF FOLLICLE WAVE SYNCHRONIZA
ONIZATION AND ECG TREATMENT ON CUMULUS US OOCYTE COMPLEX RECOVER
OVERY
RATES AND QUALIT
ALITY IN BRANGUS AND ANGUS DONORS
Felipe Ledur Ongaratto 1 , Paula Rodriguez Villamil 1 , Andres Tribulo 1 & Gabriel A. Bo 2
1
INSTITUTO DE REPRODUCCION ANIMAL, CORDOBA, ARGENTINA. 2 IRAC - UNVM, ESTRANGEIRO, ARGENTINA.
Two experiments were designed to evaluate the effect different treatments of follicle wave synchronization and superstimulation
on the number and quality of COCs aspirated per OPU session. Experiment 1 was designed to test the hypothesis that synchronizing the
emergence of follicular wave using estradiol benzoate (EB) and P4 would improve the number and quality of oocytes aspirated by OPU.
Brangus (n = 10) and Angus (n = 10) cows were randomly allocated in two treatment groups and treated two times in a cross-over design. On
Day 0, donors in Group 1 received 2.5 mg EB (Zoovet, Argentina) and 50 mg P4 (Syntex, Argentina) intramuscularly (im); whereas, donors
in Group 2 (Control) did not receive any hormonal treatment. On Day 6, OPU was performed by ultrasound-guided follicular aspiration and
COCs were classified based on cytoplasm appearance and cumulus cell numbers as described by Chaubal et al. 2006. Data was analyzed by
ANOVA. The mean number of follicles aspirated on Day 6, total number of oocytes retrieved and the number of viable oocytes were higher
(P < 0.05) in cows in Group 1(12.9 ± 1.1; 7.9 ± 1.3 and 5.2 ± 0.9) than in those in Group 2 (7.3 ± 0.7; 3.2 ± 0.5 and 2.1 ± 0.4). Experiment
2 was designed to test the hypotheses that: 1) induction of luteolysis 3 days prior to aspiration (to avoid the presence of the CL at the time
of OPU) and 2) treatment with eCG (to stimulate follicle development), would improve the number and quality of oocytes aspirated by OPU.
Brangus (n = 20) cows were randomly allocated into 3 treatment groups and treated 3 times in a cross-over design. On Day 0, all donors
received 2.5 mg EB and 50 mg P4 im (Lab. Rio de Janeiro, Argentina). On Day 4, donors in Group 1 received 150 µg D(+) cloprostenol (PGF,
Ciclar, Zoovet, Argentina) and donors in Group 2 received PGF plus 800 IU eCG (Novormon, Syntex); whereas donors in Group 3 did not
receive any treatment. OPU was performed on Day 7 and COCs were classified and data analyzed as in Experiment 1. The number of follicles
aspirated on Day 7, total number of oocytes retrieved and the number of viable oocytes were higher (P < 0.05) in cows in Group 1 (11.4 ±
1.2 e 7.9 ± 1.1) than in those in Groups 2 (7.5 ± 1.4 e 4.5 ± 1.0) and 3 (7.1 ± 0.8 e 4.7 ± 0.7). In Conclusion, synchronization of follicle wave
emergence and the induction of the luteolysis prior to OPU, but not the superstimulation treatment with eCG positively affected the number
and quality of COCs.
Keywords: emergence wave synchronization, ecg, oocyte.
A132 OPU-IVP AND ET
EFFECT OF FSH INCORPOR
ORPORATED IN A POLIMERIC MATRIX OF PLUR
URONIC F-127 ® ABOUT THE QUALIT
ALITY OF OOCYTES
COLLECTED BY FOLLICULAR ASPIRATION IN ZEBU HEIFERS
Hector Javier Narvaez, , Reginaldo Silva Fontes, Gina Marcela Mican, Célia Raquel Quirino
UNIVERSIDADE ESTADUAL DO NORTE FLUMINENSE DARCY RIBEIRO, CAMPOS DOS GOYTACAZES, RJ, BRAZIL.
This study aimed to evaluate the effect of slow release of FSH when incorporated in a biodegradable polymer matrix of pluronic
F-127 ® on the quality of oocytes recovered by transvaginal follicular aspiration (OPU) in Nelore x Brahman heifers. Eight zebu heifers were
used (Bos indicus), as oocyte donors age between 20 to 24 months. In a random day of the estrous cycle heifers were pre-synchronized by
the association of an intravaginal gadget containing 1g of progesterone (Sincrogest ® , Ouro Fino, Brazil ) plus 2mg of estradiol benzoate (RIC-
BE ® , Tecnopec, Brazil). After 8 days, the intravaginal progesterone gadget was removed, we administered 150 µg D-Cloprostenol (Prolise ® ,
Tecnopec, Brazil) and 24 h later all females received an application of 1mg estradiol benzoate. 8 days after pre-synchronization heifers received
a 150µg dose of D-cloprostenol to eliminate the luteum corpus present and 36 h after animals were subjected to the aspiration of follicles >5mm
diameter, in order to synchronize the emergence of a new follicular wave, and considered as D0. As this moment, females were assigned to two
experimental groups. In the control group (G1) (n = 4), animals didn’t receive the polymer matrix of pluronic F-127 ® FSH. Group 2 (n = 4),
animals that received the polymer matrix of pluronic F-127 ® containing 50mg de FSH-p (Folltropin-V ® , Bioniche, Canada) subcutaneously. The
animals in both experimental groups were subjected to three replicas with an interval of 10 days, including the synchronization of the wave with
puncture of follicles >5mm and the OPU performed 5 days after this synchronization procedure. For the preparation of the polymeric matrix was
used pluronic F-127 ® 20% (Sigma -Aldrich chemical Co, USA) and 1% de PVA (Sigma-Aldrich Chemical Co, USA) diluted in PBS was later
performed the addition of FSH, the polymer matrix as it was cooled to 4°C use. Analysis of variance was performed using the SAS statistical
program. There was statistical difference (P < 0.05) for the average number of follicles seen in G2 (24.7±14.3) compared to G1 (15.1±7.5).The
number oocytes retrieved, the G1 to G2 was higher (14±9.4 and 7.9±4.2, respectively). Regarding the quality oocytes, the G2 had more grade
I structures (5.8±3.2) compared to G1 (1.2±0.7). And the total number of viable oocytes was significant for G2 compared to G1 (13.8±6.5 and
7.4±2.1). It can be concluded that the application of FSH incorporated in a polymer matrix of pluronic F-127 ® was effective in improving the
quality of oocytes picked.
Keywords: opu, fsh, pluronic f-127 ®
s402