2011 (SBTE) 25th Annual Meeting Proceedings - International ...

Acta Scientiae Veterinariae, 2011. 39(Suppl 1): Abstracts - 25th Annual Meeting SBTE-Brazil. August 2011.
A049 FOLLICULOGENESIS, OOGENESIS AND SUPEROVULATION
THE EFFECT OF ECG IN MULTIPLE OVULATION PROTOC
OCOLS OLS FOR HIGH PRODUCTION HOLSTEIN COWS EMBRYO DONORS
Carlos Alberto Rodrigues 1 , Lais Mendes Vieira 2 , Andressa Lavezzo Ranieri 3 , Pericles Ricardo Lacerda e Silva 4 , Henderson Ayres 5 & Pietro Sampaio Baruselli 6
1,4
SAMVET, SAO CARLOS, SP, BRAZIL. 2,6 FMVZ USP, SÃO PAULO, SP, BRAZIL. 3 FCAV UNESP, JABOTICABAL, SP, BRAZIL. 5 INTERVET SCHERING-PLOUGH, BAURU, BULGÁRIA.
The aim of this study was to evaluate the multiple ovulation response (number of corpora lutea – CL and number of viable
embryos) in high production Holstein cows treated with eCG in the end of the protocol for multiple ovulation (MO) with FSH. The study
was conducted in two periods (30 to 40 days interval). A total of 23 donors were used, whereas all donors passed through the two treatments,
ending up with 46 MO. The cows were allocated in two experimental groups, according to the administration or not of eCG. Cows from group
FSH (n = 23) received a norgestomet ear implant (Crestar ® , Intervet, Brazil) in the morning (AM) of a random day of the estrous cycle (D0)
+ 2 mg estradiol benzoate (Gonadiol ® , Intervet, Brazil) + 50 mg progesterone (Index, Brazil) IM. From D4 on, each animal received 500 IU
of FSH (Pluset ® , Hertape Calier, Brazil), diluted in 20 mL and divided in 8 applications of decreasing doses (100 IU, 100 IU, 75 IU, 75 IU,
50 IU, 50 IU, 25 IU and 25 IU) 12h apart. On D6, 0.150 mg of sodic cloprostenol (Preloban ® , Intervet, Brazil) was administered AM and PM.
On D7 PM, the implant was removed and on D8 PM 200 µg Gonadorelin (Fertagyl ® , Intervet, Brazil) IM, was administered. The artificial
inseminations were done in fixed time on D9 AM and PM; embryo collection was performed on D15. Cows treated with eCG (n = 23)
received similar treatment, except for the FSH (Pluset ® , Hertape Calier, Brazil) dilution, 18mL divided in 6 application for decreasing doses:
111 IU, 111 IU, 83 IU, 83 IU, 56 IU and 56 IU. Also, a 500 IU of eCG (Folligon ® , Intervet, Brazil) was administrated on D7 AM. The number
of formed CL was registered previously to each embryo collection. The binomial and continuous variables were analized by PROC
GLIMMIX of SAS. Treatment with eCG had no effect on number of viable embryos (2.5 ± 0.7 vs. 3.5 ± 1.1; P = 0.10) per donor (average
± SE); treatments without and with eCG, respectively. Although the eCG treatment effected the number of CL (6.4 ± 0.9 vs. 8.5 ± 1.1; P =
0.01) and unfertilized oocytes rate - unfertilized oocytes per recovered structures – (34.8 ± 7.9 vs. 40.2 ± 9.0; P < 0.01) per donor (average
± SEM); treatments without and with eCG, respectively. In conclusion, the addition of 500 IU of eCG in the end of the MO protocols with
FSH in high production Holstein cows had effect on the protocol response, but did not enhance the number of viable embryos. [Acknowledgments:
Fazenda Santa Rita (Agrindus) and Intervet Schering-Plough].
Keywords: bovine, moet, ecg.
A050 FOLLICULOGENESIS, OOGENESIS AND SUPEROVULATION
EFFECT OF ACTIVIN-A ON THE DEVEL
VELOPMENT OF BOVINE SECONDAR
ONDARY FOLLICLES IN VITRO CULTURED
José Renato de Sousa Passos, , Anderson Weiny Barbalho Silva, Gisvani Lopes de Vasconcelos, Maria Juliane Passos, José Jackson do Nascimento Costa,
Rodr
drigo Otávio Dec
ecar
aria de Salles Rossi,
Francisc
ancisco Taia Gomes Bezer
erra,
Joiane Araujo da Por
orciuncula
& José Rob
ober
erto Viana Silv
ilva
UNIVERSIDADE FEDERAL DO CEARA, SOBRAL, CE, BRAZIL.
Recent studies have evaluated the effect of various hormones and growth factors during the in vitro culture of secondary follicles
and, currently, in vitro oocyte maturation and embryo production was achieved in goats (Magalhães et al., Reprod. Domest. Anim., 46:134-140,
2010). Despite the success of these studies, in vitro maturation and fertilization of oocytes from preantral follicles isolated and in vitro cultured
has low efficiency, with a low rate of embryo production. In this context, the present study was conducted to verify the effect of Activin-A
associated or not with FSH in the in vitro development of bovine preantral follicles. For this, secondary follicles (~0.2 mm) were mechanically
isolated from ovaries (n = 20) and cultured in incubator with 5% CO2 in air at 39°C for 6 days in α-MEM (pH 7.2-7.4) supplemented with BSA
(3.0 mg/mL), ITS (insulin 10 µg/mL, transferrin 5.5 µg/mL and selenium 5 ng/mL), glutamine (2 mM), hypoxanthine (2 mM), ascorbic acid (50
µg/mL) under mineral oil. The treatment consisted of Activin-A (100 ng/mL), FSH (100 ng/mL) or the combination of FSH and Activin-A in
the same concentrations. Data on follicular diameter were analyzed by Kruskal-Wallis test (P < 0.05). Throughout the culture period, no
morphological signs of degeneration were observed. In all treatments, after 6 days of culture, a significant increase in follicular diameter (MEM:
198.77±6.67 (P = 0.2828); Activin-A: 225.46±8.67 (P = 0.0052); Activin-A + FSH: 211.81±8.24 (P = 0.0203); FSH: 236.75±11.20 (P =
0.0099), was observed when compared to day 0 (MEM: 182.64 ± 6.98; Activin-A: 187.17±11.85; Activin-A + FSH: 174.98±9.85; FSH:
180.56±9.27). In addition, at the end of culture, Activin-A significantly increased follicular diameter (225.46±8.67) when compared with day 2
(197.10±12.10). After 6 days, when comparisons among treatments were performed Activin-A + FSH (211.81±8.24) and FSH (236.75±11.20),
significantly increased follicular diameter compared to MEM alone (198.77±6.67) (P = 0.0052). In conclusion, this study demonstrated that
Activin-A and FSH promotes the growth of bovine secondary follicles in vitro.
Keywords: follicle, activin-a, culture.
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