2011 (SBTE) 25th Annual Meeting Proceedings - International ...

Acta Scientiae Veterinariae, 2011. 39(Suppl 1): Abstracts - 25th Annual Meeting SBTE-Brazil. August 2011.
A141 OPU-IVP AND ET
INFLUENCE OF THE SPERM PRE-INCUBATION ON CLEAVAGE RATES,
BLAST
ASTOCY
OCYST PRODUCTION AND SEX DISTRIBUTION OF
IN VITRO PRODUCED BOVINE EMBRYOS
Alinne Gloria Curcio, Reginaldo Silva Fontes, Steveen Ribeiro Leal, Célia Raquel Quirino, Gina Marcela Mican, Carla Sobrinho Paes de Carvalho & Gonçalo
Apolinario de Souza Filho
UENF, CAMPOS DOS GOYTACAZES, RJ, BRAZIL.
Studies on bovine embryos produced in vitro have showed that the sex ratio may differ from 1:1. This works suggest that Y-
chromosome-bearing spermatozoa have a selective advantage for early fertilization (Kochhar et al. Animal Reproduction Science 77 (2003) 33–
49). On the other hand, X-chromosome-bearing show greater longevity and penetrate the majority of oocytes when they become ready for
fertilization (Dominko and First, Theriogenology 47(1997), 1041–1050). The objective of this study was to determine the influence of bovine
sperm pre-incubation in the IVP and the sex ratio of bovine embryos on days 8 and 9 of development. A group of 277 oocytes from
slaughterhouse cows from Campos dos Goytacazes, RJ were matured for 22h in modified TCM199 and in vitro fertilizated with frozen-thawed
semen bull. Prior the fertilization, the sperm were pre-incubated in Fec-Talp supplemented with heparin for 1 h (G-1) or 4 h (G-4) in a humidified
atmosphere of 5% CO2 in air at 38ºC in IVF droplets adjusted to a final concentration of 1x106/mL. In the control group (GC) there wasn´t
sperm pre-incubation. The IVC was done in TCM199 supplemented with FSB 10%. All the experiment was repeated for five times. All reagents
used were obtained from Sigma (St. Louis, USA) Cleavage and blastocyst production rates were analyzed on the days 3 and 7-8 of development
respectively, by ANOVA test (SAS, 2002). Blastocysts at different development stages on days 8 e 9 were sexed by amplification of bovine
amelogenine gen (Leal S.R. (2007), Dissertation (UENF). There were no significant differences in cleavage rates between the groups G-1, G-
4 and CG (59,40 ± 18,82; 43,60 ±17,76; 63,80 ± 10,03, respectively), but the sperm pre-incubation for 1 h increased the rate of blastocyst
production (37,20 ±19,43) when compared with CG (28,20 ± 15,75) and G-4 (11,00 ± 5,83). More than 90% of embryos were successfully
sexed. The sperm pre-incubation did not lead to a significant alteration (X2, P > 0,05) in the sex ratio (M:F) among experimental groups (GC:
0,90; G-1: 1,15; G-4: 0,8). The sperm pre-incubation for one hour increased the IVP, but did not change the sex proportion of embryos collected
on eight or nine day of development. In this way, further studies should be performed to determine how these factors act on developmental
competence and in the sex distribution of in vitro produced bovine embryos.
Keywords: in vitro production, oocyto, amilogenine.
A142 OPU-IVP AND ET
INFLUENCE OF SEMEN ON THE PREGNANCY RATE IN MARES UNDER EMBRYO TRANSFER IN THE NORTHEAST
OF THE STATE TE OF PAR
ARA
S ebastião Tavar
ares Rolim Filho
,
Fr anklin Oliv
liveir
eira Barb
arbosa Filho
ilho, Haroldo Fr ancisco Loba
obat o Rib
ibeir
eiro, Ellen
Yasmin Eguchi Mesquita,
Keitiane Colares Sousa & Henry Daniel Manrique Ayala
CURSO DE PÓS-GRADUAÇÃO EM CIÊNCIA ANIMAL, UNIVERSIDADE FEDERAL DO PARÁ (UFPA), BELEM, PA, BRAZIL.
The aim of this study was to evaluate the pregnancy rate of mares recipients transferred with embryos from donor inseminated with
semen of different types in the northeastern state of Para, assessing such variables as influenced by the type of semen on conception rate of
donors. 120 mares were used, which were 43 donor embryo, pure breeds of Quarter Horse and Paint Horse (with age ranging from 3 to 18 years)
weighing between 350 and 600 kg were selected for high genetic value and excellent characteristics for livestock or skills in equestrian events
and 77 of embryo recipient mares, breed aged between 3 and 10 years, weighing between 350 and 500 kg being performed gynecological
examinations and evaluation of reproductive history. 16 stallions were used, since they are the breeds Quarter Horse and Paint miles, with age
ranging between 3 and 20 years, with different types of semen (frozen, fresh and chilled). The frozen semen was imported from other regions
of the country and transported and stored in liquid nitrogen canister. Insemination with fresh semen were taken immediately after collection.
Already cooled semen was diluted with the diluent “Max Semen” in a 2:1 ratio and transported in appropriate containers “Equitainer” to 5°C for
24 h. Also cooled semen was used from the southeast and northeast. In this study we found a conception rate of 88.13%, 68.42%, 73.07%, using
fresh semen, cooled and frozen, respectively. Significant difference in conception rate from the donor, using fresh and cooled semen (P < 0.05)
did not differ in relation to frozen semen (P > 0.05). The conception rate in donor insemination with cooled semen was lower than the results
obtained with fresh semen and frozen, assuming failure in quality shipping chilled semen, which can interfere with sperm quality.
Keywords: mares, embryo transfer, semen.
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