2011 (SBTE) 25th Annual Meeting Proceedings - International ...

Acta Scientiae Veterinariae, 2011. 39(Suppl 1): Abstracts - 25th Annual Meeting SBTE-Brazil. August 2011.
A081 FTAI, FTET AND AI
EVAL
ALUATION OF ARTIFICIAL INSEMINATION IN GIROL
OLANDO HEIFERS WITH SEXED SEMEN
Marcio Ferreira Mendanha, , Silvia Ferrari & Daniela Stockmann Silva
UNIVERSIDADE ANHEMBI-MORUMBI, SÃO PAULO, SP, BRAZIL.
The objective of this experiment was to compare three protocols of estrus synchronization and artificial insemination with sexed
semen and also evaluate the use of artificial insemination in the uterine horn. Virgin Girolando heifers (n = 45) selected based on their body score
(2,5 – 3,5) weight (2/3 of adult weight) and age (18 – 24 months) were enrolled in this study. The cyclicity of heifers was confirmed through
trans-rectal ultrasound exams. The animals were divided in three groups of 15. The cows in AIEstr treatment ( AIEst- artificial insemination on
estrus) received 2 mL of Cloprostenol on day one and on day 11. Observation of estrus occurred three times a day and the artificial insemination
was done 12 h after observation of estrus. The AITF(artificial insemination on fixed time) protocol was provided by Ourofino company. Cows
enrolled in the AITF treatment received an intravaginal device with 1g of progesterone and 2 mL of benzoate of estradiol on day zero. After 8 days
the devices were withdrawn and 400 UI of eCG and 0,5mL of ciprionato of estradiol were administered on the animals. On day 10, 2,5 mL of
GnRH were administered and the cows were inseminated. Cows enrolled in the AITFUS treatment received injections of 2 mL of clorprostenol
until the follicles reached 14mm, at which time cows received 2,5 mL of GnRH, followed 4 h later by artificial insemination deep in the
ipsilateral horn to the ovary with the largest follicle. Similar pregnancy rates were observed at 45d after AI (AIEst =55%, AITF=40%, and
AITFUS= 55%). Cows enrolled in the AIEstr treatment showed more variations within the estrus. In conclusion, the pregnancy rates were
similar between treatments and acceptable pregnancy rates were obtained in all of them. The technique that uses GnRH hormone as ovulation
inductor with artificial insemination in the uterine horn might be used obtaining pregnancy rates similar to the other tested protocols.
Keywords: sexed semen, girolando heifers, ai.
A082 FTAI, FTET AND AI
EVAL
ALUATION OF DIFFERENT DOSES OF ECG IN THE TAI CONCEPTION RATE IN CYCLING CLING NELORE HEIFERS SYNCHRONIZED
WITH INTRAVAGINAL PROGESTER
OGESTERONE ONE DEVICE PREVIOUSL
VIOUSLY USED FOR 9 DAYS.
Everton Rodolfo Carvalho 1 , Adnan Darin Pereira Rodrigues 2 , Thiago Martins 2 , Hugo Borges Graff 1 , Rogério Fonseca Guimarães Peres 1 & Amanda Prudêncio
Lemes 3
1
AGROPECUÁRIA FAZENDA BRAZIL, BARRA DO GARÇAS, MT, BRAZIL. 2 UNESP, BOTUCATU, SP, BRAZIL. 3 ESALQ-USP, PIRACICABA, SP, BRAZIL.
The TAI in beef cows has equivalents results to conventional AI, however, in nelore heifers the results with the TAI are lower
when compared to conventional AI (Dias, C. C. et al., 2009, Theriogenology, 72:378-385). The use of eCG increase the diameter of the N
dominant follicle, ovulation rate, P4 concentration after TAI and conception rate in heifers synchronized with intravaginal progesterone
implant (CIDR ® , Pfizer Animal Health, Brazil) (Peres et al., 2009, Theriogenology, 72:681-689) and norgestormet ear implant (Crestar ® ,
Intervet Schering Plough) (Sá Filho et al., 2010, Anim Reprod Sci, 118, 182-187). The aim of this study was determine the effect of the dose
of eCG in the conception rate in Nelore heifers synchronized with CIDR of second use. Heifers (n = 626) with average body condition score
(BCS) of 2.92, were used. The heifers was considered cycling when observed presence of CL in one of two ultrasound (Mindray -DP
2200VET) evaluation on D-7 and D0 of the TAI protocol. All heifers receive the first day (D0) intravaginal progesterone implant (CIDR ® ,
Pfzier Animal Health, Brazil) and 2 mg estradiol benzoate (BE), after seven days (D7) 12,5 mg dinoprost trometamine (PGF2α, Lutalyse ® ,
Pfizer Saúde Animal, Brazil). On day nine (D9) 0.5 mg estradiol cypionate (ECP ® , Pfzier Animal Health, Brazil) and implant withdrawal, and
TAI was performed 48hs after (D11). On D9, the heifers were grouped randomly to receive 100 IU (n = 160), 200 IU (n = 158), 300 IU (n
= 152) or 400 IU (n = 156) of eCG. The datas were analized per logistic regression using PROC LOGISTIC in SAS ® . in SAS. There was no
effect of eCG (P = 0,9479) in the conception rate when utilized 100(38,75%), 200(36,08%), 300(36,84%) and 400 (37,18%) IU. This results
shown that even using higher doses of eCG, this has no effect on conception rate in pubertal Nellore heifers synchronized with CIDR
previously used for 9 days.
Keywords: tai, ecg, heifers.
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