2011 (SBTE) 25th Annual Meeting Proceedings - International ...

More documents

Recommendations

Info

Acta Scientiae Veterinariae, 2011. 39(Suppl 1): Abstracts - 25th Annual Meeting SBTE-Brazil. August 2011. A167 EMBRYOLOGY, BIOLOGY OF DEVELOPMENT AND PHYSIOLOGY OF REPRODUCTION IMMUNOHISTOCHEMIC OCHEMICAL EVAL ALUATION OF COLL OLLAGEN I AND III IN CERVIX AND UTERUS US FROM BITCHES WITH OPEN OR CLOSED PYOMETRA Rodrigo Volpato, Ian Martin, Camila Louise Ackermann, Miriam Harumi Tsunemi, Reneé Laufer Amorim & Maria Denise Lopes UNESP, BOTUCATU, SP, BRAZIL. Canine Pyometra is defined as the accumulation of purulent secretions in the uterine lumen of adult and or older bitches. It is presented as a closed or open form and normally occurs in the luteal phase of the estrous cycle (Ângulo, 2009. Proceeding of the SEVC, Barcelona, available in www. ivis.org). Recently Chatdarong et al. (2010. Proceedings 9th Chulalongkorn University Veterinary Science the Animal Company. v.1, p.129) concluded that the uterus of cyclic bitches have a higher proportion of collagen fibers compared with smooth muscle, probably associated with E2 increased during estrus, causing relaxing of the cervix. Similar results were seen in dogs with open cervix pyometra, suggesting that the opening of the cervix in these cases is associated with an increased ratio of collagen and muscle fibers. Currently the origin of cervical relaxation in cases of open pyometra is unknown. The aim of this study was to evaluate the mechanism of cervical opening and closing in bitches with pyometra, by assessing immunohistochemistry for collagen I and III in the cervix and uterus of bitches with pyometra open or closed. Fragments of uterus and cervix from bitches with open (n = 25) and closed (n = 6) pyometra were collected after ovariohysterectomy, fixed in 10% buffered formalin for 24 h and kept in 70% alcohol until the time of inclusion in paraffin. The cuts and deparaffinization were performed according Volpato et al. (Anais do XIX CBRA, 2011.). Antigen retrieval was performed with 1% pepsin pH 1.8 (Pepsin 1:1000 NF Nuclear-SP-Brazil), hatching in an incubator at 60°C and then at 37°C. Endogenous peroxidase and skimmed milk powder 3% was used as block. The incubation was carried out with primary antibody at a dilution of 1:2000 for collagen I and III (respectively Rabbit anti bonvine col I IgA Novotec. Ref. 20121; Rabbit anti bovine col III IgA Novotec. Ref. 2930 - Dako - USA ) and revelation chromogen DAB (3,3 ‘- diaminobenzidine - Liquid DAB Chromogen ® - Dako, USA). To evaluate the immunostaining, ten fields in the stroma and muscle tissues from both uterus and cervix were observed under light microscope. It was used a score from 1 to 4 to determine the percentage markup: (1 75%). Final score was the average of the scores of the 10 fields analyzed. For the statistical analysis the Mann-Whitnney test was performed (P < 0.05). There was no statistical difference in the immunostaining in any of the regions studied between the groups opened and closed pyometra.The cervical opening in cases of open pyometra is not associated with collagen receptor increase or decrease in the uterine horns and cervix of bitches. Keywords: immunohistochemical, collagen i and iii, pyometra. A168 EMBRYOLOGY, BIOLOGY OF DEVELOPMENT AND PHYSIOLOGY OF REPRODUCTION ULTR TRASONOGR ASONOGRAPHIC APHIC EVAL ALUATION OF THE CRIOLLO O CLONED EQUINE PLACENT CENTA Andres Gambini 1 , Javier Jarazo 1 , Florencia Karlanian 1 , Adrian De Stefano 1 , Cesar Bergadá 2 & Daniel Salamone 1 1 FACULTAD DE AGRONOMÍA UBA, BUENOS AIRES, ARGENTINA. 2 HOSPITAL EQUINO KAWELL, BUENOS AIRES, ARGENTINA. Cloned pregnancies are less capable to continue development after implantation. Alterations in placental development of these animals are considered one of the main reasons of low parturition rates. The combined utero-placental thickness (CUPT) can be measured by transrectal ultrasonography to monitoring an ongoing placenta. An increase in the CUPT is considered one of the main signs of placentitis. The objective of this study was to record equine clones placental development by CTUP measurement, at a monthly interval, starting at the 4th month of gestation. Five Criollo pregnant mares (A, B, C ,D and E) with good perineal conformation derived from zona-free blastocyst produced by embryo aggregation at Day 0 (Gambini et al., 2010; Reprod Fertil Dev, 23, 166) were examined. All mares received oral progesterone since pregnancy diagnosis until day 320, unless abortion took place. At each examination, the CUPT values were obtained by transrectal ultrasonography (5 MHz linear array probe, Aloka 500) in three different sections at the placenta-cervical junction, and the largest measurement was recorded. A total of eight pregnancies were achieved, but only five could develop placenta and, therefore, were evaluated in this work. CTUP values were between 0.5 and 1.3 cm. In mares A, C, D and E, CTUP were below 1 cm. Although some measurements were larger than previous reports in Criollo mares, no other abnormal clinical sings were detected. The largest CTUP was found in mare B in the sixth month of gestation, suggesting the presence of placentitis, supported with premature udder development and post-abortion placenta examination. This mare aborted, even though the CUPT decreased due to the treatment with antibiotics, anti-inflammatory and pentoxifylline. Abortion also occurred in mare D (at month 8) and E (at month 5) without detectable changes in CUPT. Mare C CUPT increased until 8th month and then remained without greater changes until parturition. This pregnancy resulted in the birth of a healthy clone. The pregnancy of mare A is still ongoing with a expected CUPT at the 8th month. These preliminary results showed that CUPT values could help in monitoring cloned placenta development. An increase higher than 1 cm may suggest a sign of compromised pregnancy, allowing the beginning of an early therapy. Abortion could also have other causes without changes on CUPT and, therefore, others clinical and echografic evaluations are necessary. Keywords: equine, clone, placenta. s420
Acta Scientiae Veterinariae, 2011. 39(Suppl 1): Abstracts - 25th Annual Meeting SBTE-Brazil. August 2011. A169 EMBRYOLOGY, BIOLOGY OF DEVELOPMENT AND PHYSIOLOGY OF REPRODUCTION CHIMERIC MOUSE BLAST ASTOCY OCYST STS S RECONSTR ONSTRUCTED BY INNER CELL MASS AND TROPHECT OPHECTODERM ODERM AGGREGA GGREGATION Isabele Picada Emanuelli, Caio Pontes Godói, Bruna Castilho Soto Campanha, Pablo Diego Moço, Bruno Cazari, Patrícia Villela Silva & Marcelo Fábio Gouveia Nogueira UNESP, BOTUCATU, SP, BRAZIL. The efficiency of embryonic chimerism tends to decrease when embryos in advanced stages of development, i.e. post-compaction, such as morula and blastocysts, are used1. To perform the inner cell mass (ICM) transfer to a recipient trophectoderm (TE) it is essential to use embryos at an advanced stage of development and with well-established blastocoels. In mice, few studies have tried the aggregation between ICM and TE and, so far, only microinjection methods have been used for such reconstructions 2,3. The aim of this study was to validate, in mice, the technique of blastocyst reconstruction using the method of ICM and TE aggregation. Swiss Webster females aged between 21 and 45 day, were superstimulated and placed to mate according to Mancini et al. (2008; Transgenic Research 17:1015). At 3.5 days post coitum (dpc), expanded blastocysts were recovered by uterine flush and were sectioned with microblade assisted by micromanipulators (NK2, Eppendorf, Germany) and mounted on an inverted microscope (Eclipse Ti, Nikon, Japan) in order to isolate ICM and TE. The section was designed to be tangential to ICM and to produce two fragments, one with just TE and another with the whole ICM and a minimum amount of the TE. The joining and subsequent aggregation were tested between pairs ICM+TE (n = 28) from different blastocysts, i.e. the ICM and TE of the same pair did not come from the same blastocyst. After joining, pairs were cultured in vitro for 24 h (37ºC, 5% CO2 and saturated humidity). The parameter used to detect a chimera in the post-culture structure was the image of a single and cohesive cell mass or a strongly aggregated mass spannning more than half of structure total diameter. The viability of the 56 sectioned structures was observed after 24 h of culture by the parameter of cellular reorganization and blastocoels re-expansion. The aggregation rate of the reconstructed blastocysts (chimerism rate) was 25% (7/28) and the viability of sectioned structures (ICM and/or TE) was 84% (47/56). Despite of low adhesion potential of embryonic cells after compaction, the proposed aggregation method for blastocyst reconstruction technique was considered feasible to the chimera production. (1) Nogueira et al.; 2010. Transgenic Research, 19:344-345. (2) Zheng et al.; 2005. Zygote, 13:73-77. (3) Sotomaru et al.; 1997. Theriogenology, 48:977-984. [Financial support: FAPESP, Brazil]. Keywords: chimeric blastocyst, embryo reconstruction, mouse. A170 EMBRYOLOGY, BIOLOGY OF DEVELOPMENT AND PHYSIOLOGY OF REPRODUCTION CHARACTERIZA CTERIZATION TION OF SECONDAR ONDARY CORPOR ORPORA LUTEA FORMATION IN NON-CYCLIC CLIC AND CYCLIC CLIC EMBRYO RECIPIENT MARES (PAR ARTIAL RESULTS) S) Elisa Sant´Anna Monteiro da Silva 1 , Sandra Figueiredo Frade 2 & Cezinande Meira 1 1 UNESP-FMVZ-BOTUCATU, BOTUCATU, SP, BRAZIL. 2 FAZENDA SÃO SABASTIÃO, MIRA ESTRELA, SP, BRAZIL. There are few reports about the characteristics of secondary corpora lutea (SCL) formation in mares and how such characteristics interfere with gestation. Therefore, the aim of the present study is to describe the morphology, number and formation pattern of SCL in pregnant embryo recipient mares, considering as hypothesis the similarity of SCL formation between cyclic and non-cyclic mares. Non-cyclic mares that received embryos during spring transition (n = 5; non-cyclic group) and cyclic mares that received embryos during breeding season (n = 5; cyclic group) were used. Prior to embryo transfer, non-cyclic mares were given a single dose of estradiol benzoate (2.5 mg, IM; EstroginTM, Farmavet, Brazil) and had their uteri examined daily by transrectal ultrasonography (US) for observation of uterine edema. Twenty four hours after detection of satisfactory endometrial edema, daily administration of altrenogest began (0.044 mg/kg, PO; ProgestalTM, Pro-Ser, Argentina). Embryos were collected by non-surgical uterine flush 8 days after ovulation of the donor mare and transferred into non-cyclic recipients 4- 6 days after the onset of altrenogest administration or into cyclic recipients 4-6 days post-ovulation. Pregnancy diagnosis was performed by US at 15 days. Non-cyclic recipients that became pregnant received altrenogest until day 120 of gestation. Additional pregnancy examinations were performed in all pregnant recipients on days 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 85 and 100. Presence of ovarian follicles, as well as formation, number and morphologic characteristics of SCL were also monitored beginning on day 35. Emergence of SCL varied among mares, ranging from days 36-40 to 61-65, and was associated with ovulation of large follicles whereas later formation of SCL originated mainly from luteinization of follicles. At 100 days of gestation luteinized structures were still present on the ovaries of some animals. A total of 2 to 6 SCL that had emerged at different periods during pregnancy were observed per mare. Regarding morphology, types of SCL observed were clot-like, solid and spherical, with anechoic center or elongated SCL projecting towards the ovulation fossa. Secondary corpora lutea vary in number and morphology among animals and their formation seem to occur similarly in recipients that became pregnant during the ovulatory season and progestin-treated transitional recipients. Data regarding the production of progesterone by these glands are necessary to clarify their role during gestation in mares. [The authors thank Fapesp for financial support]. Keywords: altrenogest, mares, non-cyclic. N s421
Page 1 and 2:
Proceedings of the 25 th Annual Mee
Page 3 and 4:
Acta Scientiae Veterinariae. 39 (Su
Page 5:
Page 8 and 9:
Page 10 and 11:
Page 12 and 13:
Page 14 and 15:
Page 16 and 17:
Page 18 and 19:
Page 20 and 21:
Page 22 and 23:
A262 Bovine Oocyte Vitrification: E
Page 25 and 26:
C.A. Rodr drigues igues, R.M. Fer e
Page 27 and 28:
Page 29 and 30:
Page 31 and 32:
Page 33 and 34:
Page 35:
Page 38 and 39:
L.F. Nasser asser, L. Pen enteado e
Page 40 and 41:
Page 42 and 43:
Page 44 and 45:
Page 46 and 47:
R.B. Lôbo, D. Nkr uman, D.A. Gross
Page 48 and 49:
R.B. Lôbo, D. Nkr uman, D.A. Gross
Page 51 and 52:
M.E.F .F. Oliv liveir eira. 2011. E
Page 53 and 54:
Page 55 and 56:
Page 57:
Page 60 and 61:
A.L. Gusmão usmão. 2011. Estado d
Page 62 and 63:
Page 64 and 65:
Page 66 and 67:
J. R. Figueir igueiredo edo, A.P.R.
Page 69 and 70:
E.L.A. Motta, M. Nichi & P.C. Ser e
Page 71 and 72:
Page 73 and 74:
Page 75 and 76:
Page 77:
Page 80 and 81:
E.L.Gastal, M.O. Gastal, A. Wischra
Page 82 and 83:
Page 84 and 85:
Page 86 and 87:
Page 88 and 89:
Page 90 and 91:
Page 92 and 93:
Page 95 and 96:
D.P .P.A.F .A.F. Braga & E. Bor org
Page 97 and 98:
Page 99 and 100:
Page 101 and 102:
Page 103:
Page 106 and 107:
F.F .F. Bressan, F. Per erecin, eci
Page 108 and 109:
Page 110 and 111:
Page 112 and 113:
Page 114 and 115:
Page 116 and 117:
Page 119 and 120:
C.E. Ambrósio mbrósio, C.V. Wenc
Page 121 and 122:
Page 123:
Page 127 and 128:
J.C. Fer erreir eira, F.S. Ignácio
Page 129 and 130:
Page 131 and 132:
Page 133:
Page 136 and 137:
R.C. Uliani, L.A. Silv ilva, M.A. A
Page 138 and 139:
Page 140 and 141:
F.S. Ignácio, J.C. Fer erreir eira
Page 142 and 143:
F.S. Ignácio, J.C. Fer erreir eira
Page 145 and 146:
L.A. Silva. 2011. Local Effect of t
Page 147 and 148:
Page 149 and 150:
Page 151 and 152:
Page 153 and 154:
Page 155 and 156:
Page 157 and 158:
M.M. Franc anco, A. Pellegr ellegri
Page 159 and 160:
M.M. Franc anco, A. Pellegr ellegri
Page 161 and 162:
B. Str troud & G.A. Bó. 2011. The
Page 163 and 164:
Page 165 and 166:
Page 167 and 168:
Page 169 and 170:
W.W .W. Tha hatcher cher. 2011. Tem
Page 171 and 172:
Page 173 and 174:
Page 175 and 176:
Page 177 and 178:
Page 179 and 180:
Page 181 and 182:
Page 183 and 184:
Page 185 and 186:
Page 187 and 188:
Page 189 and 190:
Page 191:
Page 195 and 196:
O. Sandra. 2011. Deciphering early
Page 197 and 198:
Page 199 and 200:
Page 201 and 202:
Page 203 and 204:
Page 205 and 206:
R.C. Cheb hebel. el. 2011. Use of A
Page 207 and 208:
Page 209 and 210:
Page 211 and 212:
Page 213 and 214:
Page 215 and 216:
Page 217 and 218:
Page 219 and 220:
Page 221 and 222:
Page 223 and 224:
Page 225 and 226:
Page 227 and 228:
Page 229 and 230:
Page 231 and 232:
Page 233 and 234:
Page 235 and 236:
Page 237 and 238:
Page 239 and 240:
Page 241 and 242:
Page 243:
Page 246 and 247:
A.C.A. Net eto, A.R. Galdos aldos,
Page 248 and 249:
A.C.A. Net eto, A.R. Galdos aldos,
Page 250 and 251:
P.C .Cha havett ette-P e-Palmer alm
Page 252 and 253:
Page 254 and 255:
Page 256 and 257:
Page 258 and 259:
Page 260 and 261:
Page 262 and 263:
Page 264 and 265:
Page 266 and 267:
E.H. Bir irgel Junior unior, F.V .V
Page 268 and 269:
Page 270 and 271:
Page 272 and 273:
Page 274 and 275:
Page 276 and 277:
P. Humblot. 2011. Reproductive Tech
Page 278 and 279:
Page 280 and 281:
Page 282 and 283:
Page 284 and 285:
Page 286 and 287:
J.A. Piedrahita. 2011. Application
Page 288 and 289:
Page 290 and 291:
Page 292 and 293:
Page 294 and 295:
Page 296 and 297:
O.E. Smith, B.D. Murphy & L.C. Smit
Page 298 and 299:
Page 300 and 301:
Page 302 and 303:
Page 304 and 305:
Page 307 and 308:
D. Salamone alamone, R. Bevacqua, F
Page 309 and 310:
Page 311 and 312:
Page 313 and 314:
Page 315:
Page 318 and 319:
E.A. Maga & J.D. Mur urray. 2011. G
Page 320 and 321:
Page 322 and 323:
Page 325:
Page 328 and 329:
C.G. Gutier utierrez, S. Fer errar
Page 330 and 331:
Page 332 and 333:
Page 334 and 335:
Page 336 and 337:
Page 338 and 339:
Page 340 and 341:
B. Gasparrini. 2011. Ovum pick-up a
Page 342 and 343:
Page 344 and 345:
Page 346 and 347:
Page 348 and 349:
Page 350 and 351:
Page 352 and 353:
Page 354 and 355:
Page 356 and 357:
Page 359 and 360:
Acta Scientiae Veterinariae, 2011.
Page 361 and 362:
Page 363 and 364:
Page 365 and 366:
Page 367 and 368:
Page 369 and 370:
Page 371 and 372:
Page 373 and 374:
Page 375 and 376:
Page 377 and 378:
Page 379 and 380:
Page 381 and 382:
Page 383 and 384:
Page 385 and 386:
Page 387 and 388:
Page 389 and 390:
Page 391 and 392: Acta Scientiae Veterinariae, 2011.
Page 441: Acta Scientiae Veterinariae, 2011.
Page 489: Acta Scientiae Veterinariae, 2011.
show all

2011 (SBTE) 25th Annual Meeting Proceedings - International ...

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?